Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   TK90_RS10980 Genome accession   NC_013889
Coordinates   2299297..2300508 (+) Length   403 a.a.
NCBI ID   WP_012983547.1    Uniprot ID   D3SCV2
Organism   Thioalkalivibrio sp. K90mix     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2294297..2305508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK90_RS10940 (TK90_2181) nusG 2294601..2295134 (-) 534 WP_012983544.1 transcription termination/antitermination protein NusG -
  TK90_RS10945 (TK90_2182) secE 2295151..2295516 (-) 366 WP_012983545.1 preprotein translocase subunit SecE -
  TK90_RS10955 (TK90_2183) tuf 2295718..2296908 (-) 1191 WP_012983534.1 elongation factor Tu -
  TK90_RS10975 (TK90_2184) pilB 2297518..2299236 (+) 1719 WP_012983546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TK90_RS10980 (TK90_2185) pilC 2299297..2300508 (+) 1212 WP_012983547.1 type II secretion system F family protein Machinery gene
  TK90_RS10985 (TK90_2186) pilD 2300824..2301687 (+) 864 WP_041444278.1 A24 family peptidase Machinery gene
  TK90_RS10990 (TK90_2187) coaE 2301692..2302303 (+) 612 WP_012983549.1 dephospho-CoA kinase -
  TK90_RS10995 (TK90_2188) zapD 2302454..2303224 (+) 771 WP_012983550.1 cell division protein ZapD -
  TK90_RS11000 (TK90_2189) - 2303247..2304248 (-) 1002 WP_041444279.1 Nudix family hydrolase -
  TK90_RS11005 (TK90_2190) argJ 2304229..2305440 (-) 1212 WP_012983552.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43837.47 Da        Isoelectric Point: 9.6544

>NTDB_id=36420 TK90_RS10980 WP_012983547.1 2299297..2300508(+) (pilC) [Thioalkalivibrio sp. K90mix]
MADSGTIYAWEGLNKNGDRVKGETPADSEAVARSELRKNGINVVKIRKKPKSLLSGGKKKIVPADIAYFLRQMTTMLQSG
VPLVQAFEIVGRGNEKPAMRDLVLKLKADVEGGETFAAALAKQPKYFDDLVVNLVEAGEQAGTLETLLDKVATYKEKTEA
LKAKIRKAMFYPAAVVVVAIVVTAILLIYVVPQFESLFQGFGADLPVFTRMVVDLSGFVQSWWWLILAIMVAIGFAFVET
RRRSPRFARFLDIAILKAPAFGPILRKAAIARFARTLSTMFSAGVPLVDALTSVSGATGNALYREATLRMRDETSAGAQL
QWSMRNTGVFPNMVVQMVAIGEESGSLDSMLAKVADFYEAEVDNAVDSLSSLLEPLIMVVLGVLVGGLVIAMYLPIFQMG
QVI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=36420 TK90_RS10980 WP_012983547.1 2299297..2300508(+) (pilC) [Thioalkalivibrio sp. K90mix]
ATGGCCGATTCCGGGACAATCTACGCTTGGGAAGGACTGAACAAGAACGGCGACCGGGTCAAGGGCGAAACCCCGGCCGA
CAGCGAAGCCGTCGCACGCTCCGAACTGCGCAAGAACGGCATCAATGTCGTCAAGATTCGCAAGAAGCCAAAGTCGCTAC
TCTCCGGGGGCAAGAAAAAGATCGTCCCCGCGGACATCGCCTACTTCCTGCGACAGATGACCACGATGCTGCAATCCGGC
GTGCCGCTGGTACAGGCCTTCGAGATTGTCGGGCGCGGCAACGAGAAGCCGGCGATGCGCGATCTCGTGCTCAAGCTCAA
GGCCGACGTCGAGGGCGGCGAGACCTTCGCCGCCGCGCTCGCCAAACAGCCCAAGTACTTCGACGACCTGGTGGTCAACC
TGGTCGAGGCCGGCGAGCAGGCGGGGACGCTCGAGACCCTGCTCGACAAGGTCGCCACCTACAAGGAAAAGACCGAGGCC
CTGAAGGCCAAGATCCGCAAGGCGATGTTCTACCCGGCGGCGGTCGTCGTGGTGGCCATCGTGGTGACGGCCATCCTGCT
GATCTACGTGGTCCCGCAGTTCGAATCCCTGTTCCAGGGCTTTGGCGCGGACCTGCCGGTCTTCACCCGCATGGTGGTCG
ACCTGTCGGGATTCGTGCAGTCCTGGTGGTGGCTGATCCTCGCGATCATGGTCGCGATCGGCTTCGCATTCGTAGAGACC
CGGCGGCGTTCGCCACGATTTGCACGTTTCCTCGATATCGCGATCCTCAAGGCCCCGGCCTTTGGCCCGATCCTGCGCAA
AGCCGCGATCGCCCGCTTCGCACGGACGCTGTCCACCATGTTCTCCGCCGGCGTCCCGCTGGTCGATGCCCTGACTTCGG
TCTCCGGCGCCACGGGCAACGCGCTGTATCGCGAGGCCACGCTGCGCATGCGCGACGAGACCTCGGCCGGGGCCCAGCTG
CAATGGTCGATGCGCAATACCGGCGTGTTCCCCAACATGGTCGTGCAGATGGTCGCGATCGGCGAGGAATCCGGCTCGCT
GGACTCGATGCTGGCGAAGGTGGCGGACTTCTACGAGGCCGAGGTGGACAACGCCGTGGACAGCCTCTCGAGCCTGCTGG
AACCCCTGATCATGGTCGTACTCGGCGTACTGGTCGGCGGCCTGGTGATCGCGATGTACCTGCCCATCTTCCAGATGGGT
CAGGTCATCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3SCV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.15

99.007

0.596
  pilC Acinetobacter baumannii D1279779

57.431

98.511

0.566
  pilC Acinetobacter baylyi ADP1

57.035

98.759

0.563
  pilC Legionella pneumophila strain ERS1305867

56.061

98.263

0.551
  pilG Neisseria gonorrhoeae MS11

45.75

99.256

0.454
  pilG Neisseria meningitidis 44/76-A

45.5

99.256

0.452
  pilC Vibrio cholerae strain A1552

42.928

100

0.429
  pilC Vibrio campbellii strain DS40M4

43.434

98.263

0.427
  pilC Thermus thermophilus HB27

39.055

99.752

0.39

Multiple sequence alignment