Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TK90_RS10985 Genome accession   NC_013889
Coordinates   2300824..2301687 (+) Length   287 a.a.
NCBI ID   WP_041444278.1    Uniprot ID   -
Organism   Thioalkalivibrio sp. K90mix     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2295824..2306687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK90_RS10975 (TK90_2184) pilB 2297518..2299236 (+) 1719 WP_012983546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TK90_RS10980 (TK90_2185) pilC 2299297..2300508 (+) 1212 WP_012983547.1 type II secretion system F family protein Machinery gene
  TK90_RS10985 (TK90_2186) pilD 2300824..2301687 (+) 864 WP_041444278.1 A24 family peptidase Machinery gene
  TK90_RS10990 (TK90_2187) coaE 2301692..2302303 (+) 612 WP_012983549.1 dephospho-CoA kinase -
  TK90_RS10995 (TK90_2188) zapD 2302454..2303224 (+) 771 WP_012983550.1 cell division protein ZapD -
  TK90_RS11000 (TK90_2189) - 2303247..2304248 (-) 1002 WP_041444279.1 Nudix family hydrolase -
  TK90_RS11005 (TK90_2190) argJ 2304229..2305440 (-) 1212 WP_012983552.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31139.00 Da        Isoelectric Point: 5.6868

>NTDB_id=36421 TK90_RS10985 WP_041444278.1 2300824..2301687(+) (pilD) [Thioalkalivibrio sp. K90mix]
MLGTEELLSPPWAIAVAAIFGLLIGSFLNVVIARLPVMLERGWNAEARAILEQEPPADDVPFDLLRPRSRCPHCERPIRA
YENIPVLSFLWLRGRCPGCGTAISWQYPLVEILTAVLFALTIWHFGPDTTALLALIFTGVLIAAAGIDARTTLLPDQLTL
PLLWLGLLVNIPGTFTDLQSAVIGAAAGYLVLWLIFHGFRLATGKEGMGYGDFKLLAALGAWLGWQALPVILLLASLVGA
VVGILLILLRGRDRNIPIPFGPYLAAAGWLALIWGDTLVAFYFPQGL

Nucleotide


Download         Length: 864 bp        

>NTDB_id=36421 TK90_RS10985 WP_041444278.1 2300824..2301687(+) (pilD) [Thioalkalivibrio sp. K90mix]
ATGCTCGGCACGGAAGAGCTCCTGTCGCCACCCTGGGCAATCGCGGTCGCAGCGATTTTCGGGCTGCTAATCGGCAGTTT
TCTGAACGTGGTCATCGCCCGGCTGCCGGTGATGCTGGAGCGCGGCTGGAATGCCGAGGCACGCGCGATCCTGGAGCAGG
AGCCCCCAGCGGACGACGTCCCGTTCGATCTGCTGCGTCCACGCTCGCGCTGCCCCCACTGCGAGCGACCGATCCGTGCG
TACGAGAACATCCCCGTGCTCAGCTTCCTCTGGCTGCGCGGACGCTGCCCGGGCTGCGGCACAGCCATCAGCTGGCAGTA
CCCGCTGGTCGAGATCCTCACCGCAGTCCTGTTTGCCCTGACCATCTGGCACTTCGGACCCGACACGACAGCGCTGCTGG
CGCTGATCTTCACCGGCGTCCTGATCGCCGCCGCCGGGATCGACGCGCGCACCACCCTGCTCCCGGACCAGCTGACCCTG
CCACTGCTCTGGCTGGGGCTGCTGGTCAATATCCCGGGCACCTTCACCGATCTGCAGTCGGCCGTGATCGGAGCGGCCGC
CGGTTACCTGGTGCTGTGGCTGATCTTCCACGGCTTCCGCCTGGCCACCGGCAAGGAGGGCATGGGGTACGGCGACTTCA
AGCTGCTCGCCGCGCTGGGCGCATGGCTCGGCTGGCAGGCCCTGCCGGTAATCCTGCTGCTGGCCTCGCTGGTCGGAGCG
GTGGTCGGCATCCTGCTGATCCTGCTGCGCGGGCGCGACCGCAACATCCCGATCCCGTTCGGGCCGTATCTGGCAGCCGC
CGGGTGGCTGGCGCTGATCTGGGGCGATACGCTGGTGGCGTTCTACTTCCCACAGGGGCTCTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)

Type IV leader peptidase family (Peptidase_A24)

(136-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.957

96.864

0.523
  pilD Vibrio campbellii strain DS40M4

50.719

96.864

0.491
  pilD Neisseria gonorrhoeae MS11

51.481

94.077

0.484
  pilD Acinetobacter baumannii D1279779

50.741

94.077

0.477
  pilD Acinetobacter nosocomialis M2

50.37

94.077

0.474

Multiple sequence alignment