Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TK90_RS10975 Genome accession   NC_013889
Coordinates   2297518..2299236 (+) Length   572 a.a.
NCBI ID   WP_012983546.1    Uniprot ID   -
Organism   Thioalkalivibrio sp. K90mix     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2292518..2304236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK90_RS10925 (TK90_2178) rplJ 2292671..2293192 (-) 522 WP_026148294.1 50S ribosomal protein L10 -
  TK90_RS10930 (TK90_2179) rplA 2293408..2294103 (-) 696 WP_012983542.1 50S ribosomal protein L1 -
  TK90_RS10935 (TK90_2180) rplK 2294106..2294537 (-) 432 WP_012983543.1 50S ribosomal protein L11 -
  TK90_RS10940 (TK90_2181) nusG 2294601..2295134 (-) 534 WP_012983544.1 transcription termination/antitermination protein NusG -
  TK90_RS10945 (TK90_2182) secE 2295151..2295516 (-) 366 WP_012983545.1 preprotein translocase subunit SecE -
  TK90_RS10955 (TK90_2183) tuf 2295718..2296908 (-) 1191 WP_012983534.1 elongation factor Tu -
  TK90_RS10975 (TK90_2184) pilB 2297518..2299236 (+) 1719 WP_012983546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TK90_RS10980 (TK90_2185) pilC 2299297..2300508 (+) 1212 WP_012983547.1 type II secretion system F family protein Machinery gene
  TK90_RS10985 (TK90_2186) pilD 2300824..2301687 (+) 864 WP_041444278.1 A24 family peptidase Machinery gene
  TK90_RS10990 (TK90_2187) coaE 2301692..2302303 (+) 612 WP_012983549.1 dephospho-CoA kinase -
  TK90_RS10995 (TK90_2188) zapD 2302454..2303224 (+) 771 WP_012983550.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62402.36 Da        Isoelectric Point: 4.9563

>NTDB_id=36419 TK90_RS10975 WP_012983546.1 2297518..2299236(+) (pilB) [Thioalkalivibrio sp. K90mix]
MPNTTTAPRLPGLAKRLVDAGLIAEDIAAQIAEESRAQQTPLMPLLASKGQVSEARLAEAAAEEFGLPVLDLSCFEPELL
PKDHLNEKLIQKHHALPIMQRGNRLYIAVSDPTNLGATDEFKFATGMPVEAVLVEGHKLDRLIESALSAASSLMDDLGDD
DLENLAIESGGGDDGDTSDAADAVDDAPVVRFVNKVLMDAINRKASDIHLEPFEKDFRVRFRIDGVLHEIAHPPNTLAPR
ITARVKVMSRMDIAERRVPQDGRIKLKLSRTRAIDFRVSTLPTLYGEKIVMRILDPSSTQIGIDQLGYEEHQKEAYLQAL
KRPYGMILVTGPTGSGKTVSLYTGLGILNTADRNISTAEDPSEINMPGVNQVNVNPKVGLTFASALRAFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPYNIASSILLIIAQRLARRLCPNCKEPEDLPEEVLRK
EGFTDADIQAGVTAYRAVGCDQCTNGYKGRVGIYQVMPISEEMQRGILEGYNSMQLAQQAAKEGVEDLRRAGLVKVLAGM
TSLEEVNRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=36419 TK90_RS10975 WP_012983546.1 2297518..2299236(+) (pilB) [Thioalkalivibrio sp. K90mix]
ATGCCCAACACGACAACGGCGCCCCGCCTGCCGGGTCTGGCCAAGCGTCTCGTGGACGCCGGCCTGATTGCGGAAGATAT
CGCTGCCCAGATCGCCGAGGAATCGCGCGCTCAGCAGACCCCTTTGATGCCCCTGCTCGCCAGCAAGGGGCAGGTCTCCG
AGGCCCGCCTGGCCGAGGCCGCGGCCGAGGAGTTCGGCCTCCCGGTACTGGATTTGTCGTGCTTTGAACCCGAGCTGCTG
CCCAAGGATCACCTGAACGAGAAGCTGATCCAGAAACACCACGCCCTGCCGATCATGCAGCGCGGCAACCGCCTGTATAT
CGCGGTATCCGACCCCACCAACCTGGGGGCCACCGACGAGTTCAAGTTCGCGACCGGGATGCCGGTGGAGGCCGTACTGG
TCGAGGGGCACAAGCTCGACCGCCTGATCGAAAGCGCCCTGAGCGCTGCGTCGTCGCTGATGGACGACCTGGGCGACGAC
GATCTCGAAAACCTGGCCATCGAATCGGGTGGCGGCGATGACGGCGACACTTCGGACGCCGCCGACGCGGTGGACGACGC
GCCCGTGGTGCGCTTCGTCAACAAGGTGCTGATGGACGCGATCAACCGCAAGGCGTCGGACATCCACCTCGAGCCGTTCG
AGAAGGATTTCCGCGTGCGCTTTCGCATCGACGGCGTGCTGCACGAGATCGCCCACCCGCCGAACACCCTGGCGCCACGC
ATCACCGCGCGGGTCAAGGTCATGTCGCGCATGGACATCGCCGAGCGGCGGGTCCCGCAGGATGGGCGCATCAAGCTGAA
GCTCTCGCGCACGCGAGCGATCGATTTTCGTGTCAGCACACTGCCCACACTGTACGGCGAAAAGATCGTAATGCGTATCC
TCGACCCGAGCAGCACCCAGATCGGGATCGATCAGCTCGGCTACGAGGAGCACCAGAAGGAGGCCTACCTCCAGGCGCTC
AAGCGACCCTACGGGATGATCCTGGTCACCGGGCCGACCGGCTCGGGTAAAACGGTGTCGCTGTACACCGGCCTTGGGAT
CCTGAACACGGCCGACCGCAACATCTCCACCGCGGAAGACCCCTCGGAAATCAACATGCCCGGGGTCAACCAGGTCAACG
TGAACCCGAAGGTCGGGCTGACCTTCGCCAGCGCGCTACGCGCCTTCCTGCGTCAGGATCCGGACGTGATCATGGTGGGG
GAGATCCGCGACCTCGAGACCGCGGAGATCGCGATCAAGGCGGCGCAGACAGGGCACCTGGTGCTCTCCACGCTGCATAC
CAACGATGCCCCGCAGACCCTGACCCGACTGGCGAACATGGGGGTACCCCCCTACAACATCGCCTCCTCGATCCTGCTGA
TCATCGCTCAGCGCCTGGCGCGCCGGCTGTGCCCGAACTGCAAGGAACCGGAGGATCTCCCGGAAGAGGTCCTGCGCAAG
GAGGGCTTCACCGACGCCGACATTCAGGCCGGCGTAACCGCGTATCGAGCAGTGGGCTGCGATCAGTGCACGAACGGCTA
CAAGGGGCGGGTCGGCATCTATCAGGTCATGCCGATCAGCGAAGAGATGCAGCGCGGGATCCTGGAGGGCTACAACTCCA
TGCAGCTGGCTCAACAGGCCGCAAAGGAAGGGGTCGAGGACCTGCGTCGCGCCGGCCTGGTAAAGGTTCTGGCAGGGATG
ACCAGCCTCGAGGAAGTTAACCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.45

100

0.545
  pilB Acinetobacter baumannii D1279779

53.578

100

0.537
  pilB Legionella pneumophila strain ERS1305867

52.076

100

0.526
  pilF Neisseria gonorrhoeae MS11

50.528

99.301

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

98.427

0.49
  pilB Vibrio cholerae strain A1552

54.054

90.559

0.49
  pilB Vibrio campbellii strain DS40M4

48.759

98.601

0.481
  pilF Thermus thermophilus HB27

39.405

99.825

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.21

92.483

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

41.031

91.608

0.376

Multiple sequence alignment