Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   FAH66_RS00345 Genome accession   NZ_CP039887
Coordinates   66158..66664 (-) Length   168 a.a.
NCBI ID   WP_137040112.1    Uniprot ID   Q462L5
Organism   Neisseria subflava strain ATCC 49275     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 61158..71664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAH66_RS00320 (FAH66_00320) - 61759..63177 (-) 1419 WP_137040104.1 sodium:proton antiporter -
  FAH66_RS00325 (FAH66_00325) - 63621..63980 (-) 360 WP_003684291.1 YbaN family protein -
  FAH66_RS00330 (FAH66_00330) - 63995..64591 (-) 597 WP_137040106.1 DUF1415 domain-containing protein -
  FAH66_RS00335 (FAH66_00335) - 64709..65191 (-) 483 WP_003684427.1 Lrp/AsnC family transcriptional regulator -
  FAH66_RS00340 (FAH66_00340) - 65585..66043 (-) 459 WP_137040110.1 pilin -
  FAH66_RS00345 (FAH66_00345) pilE 66158..66664 (-) 507 WP_137040112.1 pilin Machinery gene
  FAH66_RS00350 (FAH66_00350) - 66733..67032 (-) 300 WP_137040114.1 hypothetical protein -
  FAH66_RS00355 (FAH66_00355) glnD 67456..70014 (-) 2559 WP_137040116.1 [protein-PII] uridylyltransferase -
  FAH66_RS00360 (FAH66_00360) - 70034..70330 (-) 297 WP_003680870.1 helix-turn-helix transcriptional regulator -
  FAH66_RS00365 (FAH66_00365) - 70768..71208 (-) 441 WP_137040118.1 TIGR01244 family sulfur transferase -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 17515.17 Da        Isoelectric Point: 8.9472

>NTDB_id=360923 FAH66_RS00345 WP_137040112.1 66158..66664(-) (pilE) [Neisseria subflava strain ATCC 49275]
MKAIQKGFSLIELMIVIAIVGILAVIALPAYQDYTARAQVSEAILLMEGQKSAVVEYYADKGKWPKSNEEAGIADKSIIK
GKYVAQVEVGGDGVITATMKSADVNKEIAGKTVSLTPHATTAGATTGATATANGSFTWTCTTGKTSGVATKFLPSSCRPD
EEKPAGKK

Nucleotide


Download         Length: 507 bp        

>NTDB_id=360923 FAH66_RS00345 WP_137040112.1 66158..66664(-) (pilE) [Neisseria subflava strain ATCC 49275]
ATGAAAGCAATCCAAAAAGGTTTCTCCCTGATCGAATTGATGATCGTCATCGCGATTGTCGGCATCTTGGCAGTTATCGC
CCTGCCTGCATACCAAGACTACACTGCACGTGCCCAAGTTTCTGAAGCCATCTTATTGATGGAAGGTCAAAAGTCTGCTG
TTGTTGAATACTATGCAGATAAAGGCAAATGGCCTAAGAGCAATGAAGAAGCAGGTATTGCAGATAAGAGCATCATTAAA
GGTAAATATGTTGCACAAGTAGAAGTTGGTGGTGACGGTGTTATTACTGCCACAATGAAAAGTGCTGACGTAAATAAGGA
AATTGCAGGCAAAACCGTATCCCTTACTCCTCATGCCACTACTGCCGGTGCTACTACTGGTGCAACTGCAACTGCTAATG
GTTCCTTTACTTGGACTTGTACTACAGGCAAAACCAGTGGCGTAGCTACAAAATTCTTACCTTCCTCTTGCCGTCCAGAT
GAGGAGAAACCAGCAGGTAAGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q462L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

56.395

100

0.577
  pilE Neisseria gonorrhoeae strain FA1090

54.913

100

0.565
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.429
  pilA/pilA1 Eikenella corrodens VA1

42.012

100

0.423
  pilA2 Legionella pneumophila str. Paris

42.581

92.262

0.393
  pilA2 Legionella pneumophila strain ERS1305867

41.935

92.262

0.387
  pilA Ralstonia pseudosolanacearum GMI1000

36.416

100

0.375
  pilA Acinetobacter baumannii strain A118

39.744

92.857

0.369
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.037

97.024

0.369

Multiple sequence alignment