Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   E5093_RS13795 Genome accession   NZ_CP039031
Coordinates   2893999..2894544 (-) Length   181 a.a.
NCBI ID   WP_177156101.1    Uniprot ID   -
Organism   Acinetobacter indicus strain yy_1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2876066..2893916 2893999..2894544 flank 83


Gene organization within MGE regions


Location: 2876066..2894544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5093_RS13710 (E5093_13825) pilE 2876066..2876500 (-) 435 WP_177156094.1 type IV pilin protein Machinery gene
  E5093_RS15315 (E5093_13830) - 2876494..2877129 (-) 636 WP_104473453.1 type IV pilin protein -
  E5093_RS13720 (E5093_13835) - 2877134..2880985 (-) 3852 WP_177156095.1 hypothetical protein -
  E5093_RS13725 (E5093_13840) - 2880995..2881750 (-) 756 WP_171431623.1 hypothetical protein -
  E5093_RS13730 (E5093_13845) - 2881761..2882786 (-) 1026 WP_410487192.1 PilW family protein -
  E5093_RS13735 (E5093_13850) pilV 2882762..2883286 (-) 525 WP_177156096.1 type IV pilus modification protein PilV -
  E5093_RS13740 (E5093_13855) - 2883283..2883819 (-) 537 WP_177156097.1 Tfp pilus assembly protein FimT/FimU -
  E5093_RS13745 (E5093_13860) ispH 2883983..2884933 (-) 951 WP_104500732.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  E5093_RS13750 (E5093_13865) gmk 2885069..2885692 (+) 624 WP_045795273.1 guanylate kinase -
  E5093_RS13755 (E5093_13870) rpoZ 2885769..2886050 (+) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  E5093_RS13760 (E5093_13875) - 2886274..2888373 (+) 2100 WP_104498093.1 RelA/SpoT family protein -
  E5093_RS13765 (E5093_13880) - 2888445..2888828 (+) 384 WP_005180461.1 RidA family protein -
  E5093_RS13770 (E5093_13885) - 2888983..2889177 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  E5093_RS13775 (E5093_13890) bfr 2889404..2889868 (+) 465 WP_168389502.1 bacterioferritin -
  E5093_RS13780 (E5093_13895) - 2889977..2891617 (-) 1641 WP_177156098.1 PglL family O-oligosaccharyltransferase -
  E5093_RS13785 (E5093_13900) tfpZ 2891709..2892443 (-) 735 WP_177156099.1 TfpX/TfpZ family type IV pilin accessory protein -
  E5093_RS13790 (E5093_13905) - 2892501..2893970 (-) 1470 WP_177156100.1 sulfatase-like hydrolase/transferase -
  E5093_RS13795 (E5093_13910) pilE 2893999..2894544 (-) 546 WP_177156101.1 pilin Machinery gene

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 18052.71 Da        Isoelectric Point: 9.4062

>NTDB_id=356849 E5093_RS13795 WP_177156101.1 2893999..2894544(-) (pilE) [Acinetobacter indicus strain yy_1]
MKTMQKGFTLIELMIVVAIIGILAAVAIPAYQDYTVRAKVSELIVAGSSAKSAISEGFQTNGMAGVASAATSVTKGSTAA
PVSKYLKSISANTETGVITLTTTADASLPTDAQGKTIIMSPYARAAGTGSTAGANAALAAGAAGSVEWACATTTSVQAEA
RLAALTTKGTLPAKYAPSECR

Nucleotide


Download         Length: 546 bp        

>NTDB_id=356849 E5093_RS13795 WP_177156101.1 2893999..2894544(-) (pilE) [Acinetobacter indicus strain yy_1]
ATGAAAACGATGCAAAAGGGTTTCACCCTTATCGAATTAATGATTGTTGTTGCGATCATTGGTATTTTGGCTGCAGTTGC
GATTCCTGCATACCAAGATTATACAGTTCGTGCGAAAGTTTCTGAATTAATCGTTGCAGGTTCTTCTGCTAAGTCAGCAA
TTTCAGAAGGATTCCAAACTAATGGCATGGCTGGCGTAGCCTCTGCAGCAACTTCAGTAACTAAAGGTTCAACTGCTGCA
CCGGTAAGTAAATATCTAAAATCAATTAGTGCTAACACAGAAACTGGTGTAATTACATTAACTACCACGGCTGATGCAAG
CTTACCAACTGACGCGCAAGGTAAGACAATTATTATGTCACCTTATGCACGTGCTGCAGGTACAGGTTCAACTGCTGGTG
CAAATGCAGCACTTGCTGCGGGTGCAGCTGGTTCTGTTGAGTGGGCTTGTGCTACGACAACTTCAGTTCAAGCAGAAGCA
CGTTTAGCTGCATTGACTACAAAAGGCACTTTACCAGCGAAATATGCACCGTCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-181)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

44.949

100

0.492
  pilA2 Legionella pneumophila strain ERS1305867

46.328

97.79

0.453
  pilA2 Legionella pneumophila str. Paris

44.633

97.79

0.436
  comP Acinetobacter baylyi ADP1

42.697

98.343

0.42
  pilA Ralstonia pseudosolanacearum GMI1000

40.437

100

0.409
  pilE Neisseria gonorrhoeae MS11

37.766

100

0.392
  pilE Neisseria gonorrhoeae strain FA1090

37.234

100

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.333

99.448

0.381

Multiple sequence alignment