Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HNEAP_RS07915 Genome accession   NC_013422
Coordinates   1717984..1719222 (+) Length   412 a.a.
NCBI ID   WP_012824444.1    Uniprot ID   D0L137
Organism   Halothiobacillus neapolitanus c2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1712984..1724222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNEAP_RS12275 (Hneap_1577) - 1714671..1715702 (+) 1032 WP_012824441.1 MerR family transcriptional regulator -
  HNEAP_RS07905 (Hneap_1578) - 1715762..1716217 (+) 456 WP_012824442.1 PAS domain-containing protein -
  HNEAP_RS07910 (Hneap_1579) pilB 1716275..1717972 (+) 1698 WP_012824443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNEAP_RS07915 (Hneap_1580) pilC 1717984..1719222 (+) 1239 WP_012824444.1 type II secretion system F family protein Machinery gene
  HNEAP_RS07920 (Hneap_1581) pilD 1719256..1720140 (+) 885 WP_012824445.1 prepilin peptidase Machinery gene
  HNEAP_RS07925 (Hneap_1582) coaE 1720182..1720811 (+) 630 WP_012824446.1 dephospho-CoA kinase -
  HNEAP_RS07930 (Hneap_1583) - 1720814..1721254 (-) 441 WP_133484601.1 hypothetical protein -
  HNEAP_RS07935 (Hneap_1584) - 1721277..1721573 (-) 297 WP_012824448.1 hypothetical protein -
  HNEAP_RS07940 (Hneap_1585) - 1721644..1723305 (-) 1662 WP_012824449.1 sigma-54 interaction domain-containing protein -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 45250.76 Da        Isoelectric Point: 9.8201

>NTDB_id=35527 HNEAP_RS07915 WP_012824444.1 1717984..1719222(+) (pilC) [Halothiobacillus neapolitanus c2]
MAITATAKKKPKDDKIIFVYDAKNKDGKIVKGEMSAHNEMIVRAEVRRLGLTVIKVRKKPKPLFSNTASINAKDIAVLAR
QLATMMQAGLPIVQALDIMAQSAEKVPMKELISTVKNDVESGLTLADAMDKHRKYFDELFVNLVRAGEDSGSLELVLDRI
AVYKEKSESLKGKIKKALFYPIAVVVVAFIVTSILLIFVIPQFQELFQGFGATLPAFTLFVIGISNAFKTNWYWIFGGIG
FAIFLFVYVKRRSDRFNHFLDQIILKVPIFGPLTEKAAIARFARTLGTMFSAGVPLVEAMEPTAGSTGNALFKDAVMNMR
DIIAAGSPLKVAMQQSKLFPVMVVQMVAIGEETGALDTMLGKVADMYEEEVDNMVDAMSSLLEPMIMAFLGVVVGGLVIA
MYLPIFKLGSVV

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=35527 HNEAP_RS07915 WP_012824444.1 1717984..1719222(+) (pilC) [Halothiobacillus neapolitanus c2]
ATGGCCATTACAGCGACGGCAAAAAAGAAACCAAAGGACGATAAAATCATCTTCGTTTACGATGCAAAAAACAAAGATGG
CAAGATAGTTAAGGGTGAAATGAGCGCCCATAACGAAATGATTGTTCGCGCAGAAGTTCGACGACTGGGCCTGACAGTCA
TCAAAGTCAGGAAAAAGCCCAAACCTCTGTTCAGCAACACAGCATCAATCAATGCCAAAGACATCGCCGTTTTGGCACGT
CAGCTCGCCACGATGATGCAAGCTGGCCTGCCCATCGTGCAGGCATTGGACATCATGGCGCAAAGTGCCGAAAAAGTACC
CATGAAGGAGCTGATTTCCACCGTTAAAAATGACGTGGAAAGCGGTTTGACACTGGCAGATGCCATGGACAAACACCGCA
AATATTTCGATGAACTGTTCGTGAACTTGGTCCGCGCGGGTGAAGATTCAGGCTCACTGGAGCTCGTCCTCGACCGCATT
GCCGTCTATAAAGAGAAAAGTGAAAGCCTGAAAGGCAAAATCAAGAAAGCATTGTTTTATCCCATTGCCGTGGTGGTAGT
TGCCTTTATCGTTACCTCGATTCTGCTGATCTTCGTTATTCCACAATTCCAAGAGCTGTTTCAAGGATTCGGGGCCACAT
TGCCCGCGTTCACCCTGTTTGTTATCGGCATTTCCAATGCATTCAAGACAAATTGGTATTGGATTTTTGGCGGAATCGGT
TTCGCTATTTTTCTATTTGTTTACGTCAAGCGTCGATCGGATCGTTTCAACCATTTCCTCGACCAAATCATCCTGAAAGT
TCCAATTTTCGGTCCATTAACCGAAAAAGCTGCCATTGCGCGTTTTGCGCGGACACTGGGCACGATGTTCTCCGCCGGTG
TACCGCTGGTTGAAGCAATGGAACCGACGGCAGGATCGACCGGAAACGCCTTGTTCAAAGATGCCGTCATGAACATGCGC
GACATCATTGCGGCCGGTTCACCACTCAAAGTGGCGATGCAACAATCCAAGCTGTTCCCGGTCATGGTCGTACAAATGGT
GGCCATTGGCGAAGAAACCGGTGCTTTGGATACCATGCTCGGCAAGGTTGCCGATATGTATGAGGAAGAAGTCGACAATA
TGGTCGACGCCATGTCCAGCTTGCTCGAACCCATGATCATGGCGTTCCTCGGCGTCGTGGTCGGTGGTCTTGTTATTGCT
ATGTACCTGCCGATCTTCAAACTGGGTTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0L137

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

54.523

96.602

0.527
  pilC Pseudomonas stutzeri DSM 10701

51.01

96.117

0.49
  pilC Acinetobacter baumannii D1279779

50.63

96.359

0.488
  pilC Acinetobacter baylyi ADP1

50.251

96.602

0.485
  pilC Vibrio campbellii strain DS40M4

44.638

97.33

0.434
  pilC Vibrio cholerae strain A1552

43.939

96.117

0.422
  pilG Neisseria meningitidis 44/76-A

40.295

98.786

0.398
  pilG Neisseria gonorrhoeae MS11

40.404

96.117

0.388
  pilC Thermus thermophilus HB27

38.221

100

0.386

Multiple sequence alignment