Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HNEAP_RS07910 Genome accession   NC_013422
Coordinates   1716275..1717972 (+) Length   565 a.a.
NCBI ID   WP_012824443.1    Uniprot ID   D0L136
Organism   Halothiobacillus neapolitanus c2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1711275..1722972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNEAP_RS07885 (Hneap_1574) rimI 1711725..1712219 (-) 495 WP_012824438.1 ribosomal protein S18-alanine N-acetyltransferase -
  HNEAP_RS07890 (Hneap_1575) - 1712216..1712647 (-) 432 WP_012824439.1 hypothetical protein -
  HNEAP_RS07895 (Hneap_1576) - 1712672..1714255 (-) 1584 WP_012824440.1 2-isopropylmalate synthase -
  HNEAP_RS12275 (Hneap_1577) - 1714671..1715702 (+) 1032 WP_012824441.1 MerR family transcriptional regulator -
  HNEAP_RS07905 (Hneap_1578) - 1715762..1716217 (+) 456 WP_012824442.1 PAS domain-containing protein -
  HNEAP_RS07910 (Hneap_1579) pilB 1716275..1717972 (+) 1698 WP_012824443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNEAP_RS07915 (Hneap_1580) pilC 1717984..1719222 (+) 1239 WP_012824444.1 type II secretion system F family protein Machinery gene
  HNEAP_RS07920 (Hneap_1581) pilD 1719256..1720140 (+) 885 WP_012824445.1 prepilin peptidase Machinery gene
  HNEAP_RS07925 (Hneap_1582) coaE 1720182..1720811 (+) 630 WP_012824446.1 dephospho-CoA kinase -
  HNEAP_RS07930 (Hneap_1583) - 1720814..1721254 (-) 441 WP_133484601.1 hypothetical protein -
  HNEAP_RS07935 (Hneap_1584) - 1721277..1721573 (-) 297 WP_012824448.1 hypothetical protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61860.04 Da        Isoelectric Point: 6.2206

>NTDB_id=35526 HNEAP_RS07910 WP_012824443.1 1716275..1717972(+) (pilB) [Halothiobacillus neapolitanus c2]
MNPPAITGLLAALVKTSVIEPRQATEILEKSRASKESIIQTLINSGISSAKIAQSASKNFGIPQIDLDAFDTTDLPTSIV
PAKLLENGNALPLRKRGRRLTVAMADPSDLGILENYRFSTNLTIDVVVAEAQKITRLLEEASSNSMADLANLDDDLANIQ
IDEGDIAGGDGSSEDNLQDDIVVKFVTKALTDAIRRKASDIHIEPYEKIFRIRVRIDGILTEIPPPPRQLADRIVSRVKV
MSRMDISERRVPQDGRIKLKISAGRSIDFRVNSLPTLFGEKIVLRILDPTSAQLGIDSLGYEEDQKKLYMKALERPYGMI
LVTGPTGSGKTVSLYTGLNILNTMDRNISTAEDPAEIVVPGINQVNVNARVGLTFASALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNDAPQTLTRLLNMGVPGYNIASSVNLIIAQRLARRLCNNCKKVDHVPDEELVREGFKPTD
VQEGFKIFKAVGCDQCTNGYKGRVGIYQVMPVSEEMGRIIMRGGNSMELAEQSVKEGINDLRQSGLRKVKAGVTSLEELN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=35526 HNEAP_RS07910 WP_012824443.1 1716275..1717972(+) (pilB) [Halothiobacillus neapolitanus c2]
ATGAATCCTCCCGCCATCACTGGCCTATTGGCTGCACTCGTAAAAACCTCCGTCATTGAGCCGCGACAAGCGACCGAGAT
TCTCGAGAAGTCACGAGCCAGCAAAGAATCCATTATTCAGACGCTGATCAACAGCGGGATTTCTTCTGCCAAGATCGCCC
AATCGGCATCAAAAAATTTCGGTATCCCGCAGATCGACCTGGATGCATTCGACACCACCGATTTGCCCACATCCATCGTT
CCCGCAAAACTGCTTGAAAATGGCAATGCCCTCCCCCTGCGCAAGCGAGGGCGCCGTCTTACGGTCGCCATGGCCGATCC
ATCGGATCTCGGCATTCTTGAGAATTACCGATTCAGTACCAACCTCACCATTGACGTCGTCGTGGCCGAGGCGCAAAAAA
TCACCCGATTGCTTGAAGAAGCCAGCAGCAACTCCATGGCTGATCTTGCCAATCTTGACGATGATCTGGCTAATATCCAG
ATCGACGAGGGTGATATTGCGGGCGGGGACGGTTCAAGCGAGGATAATCTTCAGGACGATATTGTCGTCAAGTTCGTAAC
CAAGGCATTAACCGATGCGATCCGACGCAAGGCTTCCGACATTCACATCGAACCCTACGAAAAGATCTTTCGTATTCGAG
TGCGTATCGATGGCATTCTGACTGAAATCCCACCGCCCCCGCGACAACTGGCCGATCGTATTGTTTCCCGCGTTAAGGTT
ATGAGTCGCATGGATATTTCCGAACGACGCGTGCCACAGGATGGTCGAATCAAGCTCAAGATTTCCGCAGGACGCTCAAT
CGATTTCCGGGTCAACTCGCTGCCGACCTTGTTCGGCGAAAAAATCGTGCTGCGAATTCTCGATCCCACCAGCGCGCAAC
TCGGTATTGATTCGCTCGGATACGAAGAAGACCAGAAAAAGCTTTACATGAAAGCGCTAGAACGTCCCTATGGCATGATT
CTGGTTACCGGTCCTACCGGCTCCGGTAAGACCGTGTCGCTGTATACGGGCTTGAATATCCTCAACACCATGGATCGGAA
TATATCGACCGCGGAGGATCCTGCTGAAATCGTGGTTCCCGGCATCAATCAGGTCAATGTCAACGCCCGCGTCGGCCTCA
CCTTCGCCAGCGCACTACGTGCCTTCCTGCGTCAGGATCCCGATGTCATCATGGTCGGCGAAATCCGGGATCTCGAGACA
GCCGAAATCGCCATCAAAGCCGCTCAGACTGGTCACTTGGTGTTATCGACGCTGCACACGAACGATGCACCCCAAACGCT
GACCCGCTTATTGAACATGGGTGTGCCGGGATATAACATCGCCTCATCAGTCAATCTGATCATTGCCCAACGACTGGCCA
GGCGACTTTGCAACAACTGCAAAAAAGTAGATCATGTGCCGGACGAGGAGCTTGTGCGCGAGGGCTTCAAGCCCACCGAT
GTACAGGAGGGCTTCAAAATTTTCAAAGCAGTAGGTTGTGATCAGTGCACTAATGGATACAAAGGCCGCGTCGGTATTTA
TCAGGTGATGCCTGTCTCTGAGGAAATGGGACGAATTATCATGCGTGGTGGTAATTCAATGGAACTGGCGGAGCAGTCTG
TCAAAGAAGGAATTAACGATTTACGGCAATCCGGCTTACGCAAAGTAAAAGCCGGTGTCACCAGCCTCGAAGAACTCAAT
CGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0L136

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.166

100

0.558
  pilB Acinetobacter baylyi ADP1

55.282

100

0.556
  pilB Legionella pneumophila strain ERS1305867

51.923

100

0.526
  pilF Neisseria gonorrhoeae MS11

47.972

100

0.481
  pilB Vibrio campbellii strain DS40M4

47.527

100

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.732

97.699

0.476
  pilB Vibrio cholerae strain A1552

47.434

100

0.474
  pilF Thermus thermophilus HB27

38.198

98.23

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.345

0.375

Multiple sequence alignment