Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HNEAP_RS07920 Genome accession   NC_013422
Coordinates   1719256..1720140 (+) Length   294 a.a.
NCBI ID   WP_012824445.1    Uniprot ID   D0L138
Organism   Halothiobacillus neapolitanus c2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714256..1725140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNEAP_RS12275 (Hneap_1577) - 1714671..1715702 (+) 1032 WP_012824441.1 MerR family transcriptional regulator -
  HNEAP_RS07905 (Hneap_1578) - 1715762..1716217 (+) 456 WP_012824442.1 PAS domain-containing protein -
  HNEAP_RS07910 (Hneap_1579) pilB 1716275..1717972 (+) 1698 WP_012824443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNEAP_RS07915 (Hneap_1580) pilC 1717984..1719222 (+) 1239 WP_012824444.1 type II secretion system F family protein Machinery gene
  HNEAP_RS07920 (Hneap_1581) pilD 1719256..1720140 (+) 885 WP_012824445.1 prepilin peptidase Machinery gene
  HNEAP_RS07925 (Hneap_1582) coaE 1720182..1720811 (+) 630 WP_012824446.1 dephospho-CoA kinase -
  HNEAP_RS07930 (Hneap_1583) - 1720814..1721254 (-) 441 WP_133484601.1 hypothetical protein -
  HNEAP_RS07935 (Hneap_1584) - 1721277..1721573 (-) 297 WP_012824448.1 hypothetical protein -
  HNEAP_RS07940 (Hneap_1585) - 1721644..1723305 (-) 1662 WP_012824449.1 sigma-54 interaction domain-containing protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32498.82 Da        Isoelectric Point: 7.7071

>NTDB_id=35528 HNEAP_RS07920 WP_012824445.1 1719256..1720140(+) (pilD) [Halothiobacillus neapolitanus c2]
MISTWVGLFNESPILWLSVALILGLLVGSFLNVVIHRLPIMLEQGWRRDCQELLEIAPSTSPLVYNLITPRSTCPHCQAP
IKAWQNIPIISWLWLRGKCEHCQHAISIQYPLIELTSGLLTLLAAWHFGVSETAIAVWVFSWILLAAAIIDLKTTLLPDN
LTLPLMWLGLLLALTGFGTTVTLENAVIGAMAGYLSLWSIYWLFKLLTGREGMGYGDFKLLAALGAWLGWQQLPMVLLLS
AGVGAVVGIGMILFLKHDKRIPIPFGPYLASAGLLSYYFGNTMFKVVFGMPGTY

Nucleotide


Download         Length: 885 bp        

>NTDB_id=35528 HNEAP_RS07920 WP_012824445.1 1719256..1720140(+) (pilD) [Halothiobacillus neapolitanus c2]
ATGATCAGCACATGGGTCGGTCTATTTAATGAGTCACCGATCCTGTGGCTGTCTGTTGCTTTAATACTGGGCTTGCTGGT
CGGCAGCTTCCTCAATGTCGTTATTCATCGCCTCCCCATCATGCTGGAGCAAGGTTGGCGGCGCGACTGCCAGGAATTGC
TGGAAATTGCTCCGTCCACGAGCCCACTAGTCTATAACCTGATCACGCCGCGCTCGACTTGTCCTCACTGCCAAGCGCCA
ATCAAGGCGTGGCAGAACATCCCGATCATCAGTTGGCTATGGCTGCGCGGCAAGTGTGAGCATTGCCAACATGCAATCAG
CATCCAATACCCCCTGATTGAGCTGACGAGCGGCTTGCTTACCCTGTTGGCTGCGTGGCATTTCGGCGTATCTGAGACGG
CGATTGCAGTTTGGGTTTTCAGTTGGATTTTGCTTGCCGCTGCCATTATCGACCTGAAAACAACCCTGCTACCGGATAAC
CTAACCCTACCTCTGATGTGGCTGGGCCTACTGCTGGCTCTTACAGGATTTGGCACGACAGTGACCCTGGAGAACGCAGT
GATCGGCGCAATGGCCGGTTACCTCTCGCTCTGGAGCATTTACTGGTTGTTCAAGTTACTGACCGGGCGCGAAGGCATGG
GCTATGGTGATTTCAAGCTACTCGCCGCTCTCGGCGCATGGCTCGGTTGGCAACAACTGCCGATGGTCCTTCTGCTATCT
GCCGGCGTCGGCGCTGTTGTCGGCATCGGCATGATTCTGTTCCTCAAGCACGACAAGCGGATACCCATTCCCTTTGGCCC
TTACTTGGCAAGCGCCGGATTACTTTCCTACTATTTTGGCAACACGATGTTCAAAGTTGTTTTTGGCATGCCCGGTACTT
ACTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-249)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0L138

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.282

96.599

0.534
  pilD Vibrio campbellii strain DS40M4

53.169

96.599

0.514
  pilD Acinetobacter baumannii D1279779

48.201

94.558

0.456
  pilD Acinetobacter nosocomialis M2

48.188

93.878

0.452
  pilD Neisseria gonorrhoeae MS11

47.037

91.837

0.432

Multiple sequence alignment