Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E4T63_RS23775 Genome accession   NZ_CP038438
Coordinates   5161170..5162387 (+) Length   405 a.a.
NCBI ID   WP_098965663.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5156170..5167387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS23755 (E4T63_23755) - 5157579..5157875 (+) 297 WP_115988878.1 DUF2845 domain-containing protein -
  E4T63_RS23760 (E4T63_23760) - 5158142..5158498 (-) 357 WP_025108814.1 BON domain-containing protein -
  E4T63_RS23765 (E4T63_23765) - 5158798..5159238 (-) 441 WP_098965660.1 pilin -
  E4T63_RS23770 (E4T63_23770) pilB 5159467..5161167 (+) 1701 WP_097089222.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E4T63_RS23775 (E4T63_23775) pilC 5161170..5162387 (+) 1218 WP_098965663.1 type II secretion system F family protein Machinery gene
  E4T63_RS23780 (E4T63_23780) pilD 5162389..5163261 (+) 873 WP_096795219.1 A24 family peptidase Machinery gene
  E4T63_RS23785 (E4T63_23785) coaE 5163258..5163881 (+) 624 WP_135296584.1 dephospho-CoA kinase -
  E4T63_RS23790 (E4T63_23790) yacG 5163878..5164075 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  E4T63_RS23795 (E4T63_23795) - 5164082..5164297 (-) 216 WP_003228298.1 hypothetical protein -
  E4T63_RS23800 (E4T63_23800) - 5164363..5165052 (-) 690 WP_135296585.1 energy-coupling factor ABC transporter permease -
  E4T63_RS23805 (E4T63_23805) - 5165170..5165796 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -
  E4T63_RS23810 (E4T63_23810) - 5165793..5166320 (+) 528 WP_098965671.1 MOSC domain-containing protein -
  E4T63_RS23815 (E4T63_23815) - 5166372..5166545 (+) 174 WP_003228304.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43952.72 Da        Isoelectric Point: 10.0979

>NTDB_id=354290 E4T63_RS23775 WP_098965663.1 5161170..5162387(+) (pilC) [Pseudomonas fluorescens strain LBUM677]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSTSLLSLGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKALTYPSAVVLVAMVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISISEFLQQWWWAVLGALIAAVFGIR
HALKKSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFVMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=354290 E4T63_RS23775 WP_098965663.1 5161170..5162387(+) (pilC) [Pseudomonas fluorescens strain LBUM677]
ATGGCGGTCAAGGCAGCGAAAATCAGCATCTACGCCTGGGAAGGCACGGATCGCAAAGGCAGCAAAGTCACGGGCGAGTT
GAGCGGGCAGAACCCCGCGCTGATCAAGGCCCAACTGCGCAAGCAAGGAATCAATCCGGGCAAGGTACGGAAGAAATCCA
CCTCCCTGCTCAGCCTCGGCAAACGCATCAAGGCCCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACCATGATGAAA
GCCGGCGTACCGCTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGACAACCCGGCGATGCGCAAACTGGTAGACGA
GGTGAAGCAGGAAGTGGCCGCCGGCAACAGCTTCGCCGCCGCACTGCGCAAGAAACCGCAGTATTTCGATGAGCTGTATT
GCAACCTGGTGGATGCCGGCGAGCAATCCGGCGCCCTCGATACGTTGCTTGAGCGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCCTGACCTACCCAAGCGCCGTGGTGCTGGTGGCAATGGTCGTTACCGGGAT
TCTGCTGGTAAAAGTGGTGCCGCAGTTTCAGTCGGTATTTTCCGGCTTCGGCGCCGAATTGCCGGCCTTCACCCTGATGG
TAATCAGTATCTCGGAGTTCCTGCAGCAATGGTGGTGGGCGGTACTTGGTGCGCTGATCGCGGCGGTGTTCGGCATCCGC
CATGCGCTGAAGAAGTCCCAGGCCTTGCGCGACCGCAAGGACACCTGGCTGCTGAAGCTGCCGCTGGTGGGCACGCTGAT
GTACAAATCGGCAGTGGCGCGATTCGCCCGCACCCTGTCGACTACCTTTGCCGCTGGCGTACCGCTGGTGGAGGCACTGG
ATTCGGTGGCGGGTGCCACCGGTAACGTGGTGTTCAAACGCGCGGTGCTGCGCATCCGTCAGGACGTCGCCACCGGCATG
CAGCTGAATTTCTCGATGCGCAGCACCGGCGTCTTCCCCAACATGGCGGTGCAGATGACCGCCATCGGTGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGAGGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCGTCATGGTGGTGCTCGGGGTCATCGTCGGCGGGCTGGTCGTGGCCATGTACCTGCCGATCTTCCAG
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Acinetobacter baylyi ADP1

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilC Vibrio cholerae strain A1552

44.949

97.778

0.44
  pilG Neisseria gonorrhoeae MS11

43.781

99.259

0.435
  pilG Neisseria meningitidis 44/76-A

43.284

99.259

0.43
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment