Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E4T63_RS23770 Genome accession   NZ_CP038438
Coordinates   5159467..5161167 (+) Length   566 a.a.
NCBI ID   WP_097089222.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5154467..5166167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS23750 (E4T63_23750) - 5154631..5157579 (+) 2949 WP_135296583.1 DUF748 domain-containing protein -
  E4T63_RS23755 (E4T63_23755) - 5157579..5157875 (+) 297 WP_115988878.1 DUF2845 domain-containing protein -
  E4T63_RS23760 (E4T63_23760) - 5158142..5158498 (-) 357 WP_025108814.1 BON domain-containing protein -
  E4T63_RS23765 (E4T63_23765) - 5158798..5159238 (-) 441 WP_098965660.1 pilin -
  E4T63_RS23770 (E4T63_23770) pilB 5159467..5161167 (+) 1701 WP_097089222.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E4T63_RS23775 (E4T63_23775) pilC 5161170..5162387 (+) 1218 WP_098965663.1 type II secretion system F family protein Machinery gene
  E4T63_RS23780 (E4T63_23780) pilD 5162389..5163261 (+) 873 WP_096795219.1 A24 family peptidase Machinery gene
  E4T63_RS23785 (E4T63_23785) coaE 5163258..5163881 (+) 624 WP_135296584.1 dephospho-CoA kinase -
  E4T63_RS23790 (E4T63_23790) yacG 5163878..5164075 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  E4T63_RS23795 (E4T63_23795) - 5164082..5164297 (-) 216 WP_003228298.1 hypothetical protein -
  E4T63_RS23800 (E4T63_23800) - 5164363..5165052 (-) 690 WP_135296585.1 energy-coupling factor ABC transporter permease -
  E4T63_RS23805 (E4T63_23805) - 5165170..5165796 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62493.52 Da        Isoelectric Point: 5.8283

>NTDB_id=354289 E4T63_RS23770 WP_097089222.1 5159467..5161167(+) (pilB) [Pseudomonas fluorescens strain LBUM677]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQIGSFTIYEPVGCDLCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=354289 E4T63_RS23770 WP_097089222.1 5159467..5161167(+) (pilB) [Pseudomonas fluorescens strain LBUM677]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTCACAGGTGG
CCGAGATCGCTTCCGAGCATTTCGGTATGGCCTTCATGGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGCCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGTACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGAGGCCATCGAGAAATTCTTCGACACCCACGCCAGCGGCCTCGAAGAAATGGCCGACGTCGATCTCGACGGC
GTGGATGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATCGGCGGACTCGACGCCGATGACGCGCCGGTCGTGCGTTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGTGGCTCCTCTGACCTGCACTTCGAACCTTACGAAAAGACCTTCCGGG
TGCGGGTGCGCACCGATGGCATCCTGCGCGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGGCGAATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATCTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGACCAA
GTCCATCGACTTCCGGGTCAACACTTTGCCGACCCTATGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGCATCGATGCATTGGGTTACGAACCGGCGCAGAAAGAACTGTACATGGCAGCGCTCAAGCAGCCCCAAGGG
ATGATTCTGGTAACCGGACCTACCGGGTCGGGGAAAACCGTATCGCTGTACACCGGCCTCAATATTCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTTGCTCAGGCCCTGCGTTCGTTTCTGCGGCAGGATCCGGACGTGATCATGGTCGGGGAAATCCGCGACCTG
GAAACCGCCGAAATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACGCTGCACACCAACAGCGCTGCCGA
AACCCTGACCCGTCTGCACAACATGGGCATTCCCGGGTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCACAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCCCGCGAAACACTGATCAAGGAAGGTTTCCCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGACCTCTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTACGAAGTGGTGAAAAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.918

98.587

0.364

Multiple sequence alignment