Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E2K99_RS01885 Genome accession   NZ_CP037993
Coordinates   417578..418717 (-) Length   379 a.a.
NCBI ID   WP_134221156.1    Uniprot ID   -
Organism   Herbaspirillum huttiense strain NFYY 53159     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412578..423717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K99_RS01855 (E2K99_01855) - 413126..413551 (+) 426 WP_039790450.1 NUDIX domain-containing protein -
  E2K99_RS01860 (E2K99_01860) yacG 413594..413779 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  E2K99_RS01865 (E2K99_01865) zapD 413815..414570 (-) 756 WP_034336663.1 cell division protein ZapD -
  E2K99_RS01870 (E2K99_01870) coaE 414784..415404 (-) 621 WP_134221153.1 dephospho-CoA kinase -
  E2K99_RS01875 (E2K99_01875) - 415409..416272 (-) 864 WP_134221154.1 prepilin peptidase -
  E2K99_RS01880 (E2K99_01880) - 416326..417591 (-) 1266 WP_134221155.1 type II secretion system F family protein -
  E2K99_RS01885 (E2K99_01885) pilF 417578..418717 (-) 1140 WP_134221156.1 GspE/PulE family protein Machinery gene
  E2K99_RS01890 (E2K99_01890) - 418868..420160 (-) 1293 WP_134221157.1 HlyC/CorC family transporter -
  E2K99_RS01895 (E2K99_01895) - 420334..421296 (-) 963 WP_134221158.1 2-hydroxyacid dehydrogenase -
  E2K99_RS01900 (E2K99_01900) - 421293..422150 (-) 858 WP_039790457.1 IclR family transcriptional regulator -
  E2K99_RS01905 (E2K99_01905) - 422417..423502 (+) 1086 WP_034336645.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 40784.43 Da        Isoelectric Point: 8.2654

>NTDB_id=351138 E2K99_RS01885 WP_134221156.1 417578..418717(-) (pilF) [Herbaspirillum huttiense strain NFYY 53159]
MLHTPDTAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATRLKILARLDI
AEKRLPQDGKMRLALGARTVDFRVSTLPTQFGEKIVLRILDTAQAALSISDLGYEPAQQEALLQAISKPHGLVLMTGPTG
SGKTVSLYACLQRLNQPGVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKA
SQTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAGGTPATFVPHGCPACNQTGFRGRT
GIYQVMPVSAAMQSLILAQAGTLDLGRQAQAEGIIDLRRAGLLKVLRGETSVGEILACT

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=351138 E2K99_RS01885 WP_134221156.1 417578..418717(-) (pilF) [Herbaspirillum huttiense strain NFYY 53159]
ATGCTGCACACTCCCGACACGGCCGTGGCCGATGATGACGTCCCCGTGGTGCGCCTGCTGCACAAGCTGCTGGCCGATGC
CGTCGGCATGGGCGCTTCCGACCTGCATTTCGAGCCCTTTGAACATCTCTTCCGCGTGCGCTTGCGCCTGGATGGCGTGC
TGCACGAGCTGGCGCAGCCGCCGCTGAGTTTGCGCGACAAGCTGGCCACCCGCCTGAAGATCCTGGCGCGTCTGGATATC
GCCGAAAAGCGCCTGCCCCAGGATGGCAAGATGCGCCTGGCACTGGGCGCGCGCACCGTCGATTTCCGCGTCTCGACGCT
GCCCACGCAGTTCGGTGAAAAGATCGTGCTGCGCATCCTCGACACCGCCCAGGCGGCCTTGTCGATCAGCGACCTCGGCT
ATGAACCGGCCCAGCAGGAAGCGCTGCTGCAAGCCATCAGCAAGCCGCACGGGCTGGTCCTGATGACCGGGCCGACCGGC
TCCGGCAAGACGGTCTCGCTGTATGCCTGCCTGCAGCGGCTGAACCAGCCGGGCGTGAACATCGCCACCGCCGAGGACCC
GGTGGAAATCAACCTGCCCGGCATCAACCAGGTCAGCATCAACGAGCGCGCCGGACTGGACTTCGCCCTGGCACTGCGCG
CCTTCCTGCGCCAGGATCCGGATGTACTGATGGTGGGCGAGATCCGCGACCTGGAGACCGCCGACATCGCCGTCAAGGCT
TCGCAAACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACGCCCCGGCCACGCTCACGCGGCTGCTCAACATGGG
CGTACCGGCCTACAACATTGCGGCATCGGTCAGCCTGATCGTGGCCCAGCGGCTGGTGCGCAAGCTGTGCCGCTGCCGCC
GTCCGGCCGGTGGTACGCCGGCCACCTTCGTGCCGCACGGCTGCCCGGCCTGCAACCAGACCGGCTTTCGCGGGCGCACC
GGCATTTATCAGGTGATGCCGGTCAGCGCGGCCATGCAGTCGCTGATCCTGGCCCAGGCCGGCACATTGGATCTGGGGCG
GCAGGCGCAAGCGGAGGGCATCATCGACCTGCGGCGGGCGGGACTGCTGAAGGTCTTGCGCGGTGAAACCAGCGTGGGAG
AAATCCTTGCCTGCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(23-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.633

100

0.586
  pilB Acinetobacter baylyi ADP1

55.065

100

0.559
  pilB Acinetobacter baumannii D1279779

54.124

100

0.554
  pilB Vibrio campbellii strain DS40M4

54.712

100

0.551
  pilB Vibrio cholerae strain A1552

54.047

100

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

54.188

100

0.546
  pilB Legionella pneumophila strain ERS1305867

51.538

100

0.53
  pilB Haemophilus influenzae Rd KW20

47.215

99.472

0.47
  pilB Haemophilus influenzae 86-028NP

46.95

99.472

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

42.118

100

0.451
  pilF Thermus thermophilus HB27

43.377

100

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.837

100

0.433
  pilB Glaesserella parasuis strain SC1401

49.371

83.905

0.414
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.633

100

0.398

Multiple sequence alignment