Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MMOL_RS07585 Genome accession   NC_012968
Coordinates   1642591..1643760 (+) Length   389 a.a.
NCBI ID   WP_015832435.1    Uniprot ID   C6WWV1
Organism   Methylotenera mobilis JLW8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1637591..1648760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMOL_RS07570 (Mmol_1493) - 1639295..1639987 (+) 693 WP_041928765.1 hypothetical protein -
  MMOL_RS07575 (Mmol_1494) - 1640380..1641570 (-) 1191 WP_015832433.1 ammonium transporter -
  MMOL_RS07580 (Mmol_1495) - 1641749..1642423 (-) 675 WP_015832434.1 hypothetical protein -
  MMOL_RS07585 (Mmol_1496) pilU 1642591..1643760 (+) 1170 WP_015832435.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MMOL_RS07590 (Mmol_1497) - 1643777..1644088 (+) 312 WP_015832436.1 DUF3301 domain-containing protein -
  MMOL_RS07595 (Mmol_1498) gluQRS 1644101..1644994 (+) 894 WP_015832437.1 tRNA glutamyl-Q(34) synthetase GluQRS -
  MMOL_RS07600 (Mmol_1499) hslO 1645084..1645995 (+) 912 WP_015832438.1 Hsp33 family molecular chaperone HslO -
  MMOL_RS07605 (Mmol_1500) - 1646053..1646679 (+) 627 WP_015832439.1 LysE family translocator -
  MMOL_RS07610 (Mmol_1501) - 1646723..1647262 (-) 540 WP_015832440.1 pilin -
  MMOL_RS07615 (Mmol_1502) - 1647288..1648079 (-) 792 WP_015832441.1 TrmH family RNA methyltransferase -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43584.69 Da        Isoelectric Point: 5.6297

>NTDB_id=34773 MMOL_RS07585 WP_015832435.1 1642591..1643760(+) (pilU) [Methylotenera mobilis JLW8]
MTNEQALNYMHNLLRGMLDKKASDLFISADFPPAMKIDGKMTPVTQQKLSGEHTKAFADALMNEKQRTEFATEKECNFAI
WPKNIGRFRVNVFVQQEKVGMVLRTITTEIPKFDDLGLPDTLQDVMLTKRGLVILVGGTGSGKSTTLAALIDYRNENTFG
HIITVEDPVEYVHQSKNCLVTHREVGRDTNGWFNALKNTLRQAPDVILIGEIRDRETMEFALAFAETGHLCMATLHANSA
NQAIDRIINFFPEERHAQLHMDLSLNLKAFISQRLVSRTGGGRCAAIEILLNSPLISDLILKGETNMIKEVMAKSTELGM
QTFDQALFNLCEEGRITEDDALRNADSINELRLRFKLHGKNAGKTKSSSNFDSLSLHEDEPDESELDPT

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=34773 MMOL_RS07585 WP_015832435.1 1642591..1643760(+) (pilU) [Methylotenera mobilis JLW8]
ATGACAAACGAACAAGCACTTAATTACATGCATAACCTTTTACGTGGCATGCTTGATAAGAAAGCCTCGGATTTATTCAT
TTCCGCAGACTTTCCTCCCGCCATGAAAATTGATGGCAAGATGACACCAGTTACTCAGCAGAAACTGAGCGGTGAACATA
CCAAAGCCTTTGCTGATGCGTTGATGAATGAAAAGCAACGCACCGAATTTGCAACAGAAAAAGAATGTAATTTTGCGATC
TGGCCTAAAAATATAGGCCGCTTCCGGGTGAATGTATTTGTACAACAAGAAAAAGTCGGCATGGTGCTACGTACCATTAC
CACTGAAATTCCTAAGTTTGACGATTTAGGCCTGCCTGACACGCTGCAAGACGTAATGCTTACTAAGCGCGGGCTAGTGA
TTCTAGTAGGCGGCACCGGCTCGGGTAAGTCCACCACGCTAGCCGCGCTGATTGATTACCGCAATGAAAACACGTTTGGC
CACATCATTACCGTAGAAGACCCGGTAGAGTACGTGCACCAAAGTAAGAACTGCCTGGTCACCCATCGCGAGGTAGGTAG
AGATACCAACGGATGGTTTAACGCGCTAAAAAATACATTACGCCAAGCCCCTGATGTGATTTTAATTGGCGAAATCCGCG
ATAGAGAAACCATGGAGTTTGCGCTAGCATTTGCAGAAACCGGCCATCTTTGTATGGCCACCTTGCACGCCAACAGCGCC
AACCAAGCGATTGACCGCATCATTAACTTCTTCCCTGAAGAGCGTCACGCGCAGTTGCATATGGACTTATCGCTCAATCT
GAAAGCGTTCATCTCGCAACGTCTGGTTTCCAGAACCGGTGGCGGTCGCTGTGCTGCCATTGAAATCTTGCTTAACTCAC
CGCTGATCTCTGACCTGATTTTAAAGGGTGAAACGAACATGATTAAAGAAGTGATGGCTAAATCTACCGAGCTTGGTATG
CAAACATTTGATCAAGCCCTCTTTAATTTATGCGAGGAAGGCCGCATTACTGAAGATGATGCCCTGCGTAATGCAGACTC
AATTAACGAGTTGCGCTTACGATTTAAGTTACATGGTAAAAATGCAGGCAAGACTAAGAGTTCGTCCAATTTTGATAGCT
TGTCACTACATGAAGATGAGCCTGATGAATCCGAGCTAGACCCTACATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6WWV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.556

96.144

0.563
  pilU Acinetobacter baylyi ADP1

56.034

89.46

0.501
  pilU Vibrio cholerae strain A1552

51.762

94.859

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.881

86.118

0.378
  pilT Acinetobacter baumannii strain A118

44.375

82.262

0.365
  pilT Acinetobacter nosocomialis M2

44.375

82.262

0.365
  pilT Acinetobacter baumannii D1279779

44.375

82.262

0.365
  pilT Acinetobacter baylyi ADP1

43.519

83.29

0.362
  pilT Legionella pneumophila strain Lp02

42.857

84.576

0.362
  pilT Legionella pneumophila strain ERS1305867

42.857

84.576

0.362

Multiple sequence alignment