Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EPB57_RS19545 Genome accession   NZ_CP035700
Coordinates   2402089..2403834 (+) Length   581 a.a.
NCBI ID   WP_053307551.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2397089..2408834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS19525 pilM 2399397..2400413 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  EPB57_RS19530 pilN 2400397..2400972 (+) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EPB57_RS19535 pilO 2400965..2401558 (+) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB57_RS19540 pilP 2401548..2402063 (+) 516 WP_151514393.1 pilus assembly protein PilP Machinery gene
  EPB57_RS19545 pilQ 2402089..2403834 (+) 1746 WP_053307551.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB57_RS19550 aroK 2404022..2404540 (+) 519 WP_005381319.1 shikimate kinase AroK -
  EPB57_RS19555 aroB 2404573..2405673 (+) 1101 WP_005382764.1 3-dehydroquinate synthase -
  EPB57_RS19560 - 2405726..2407237 (+) 1512 WP_154204992.1 AAA family ATPase -
  EPB57_RS19565 - 2407307..2408146 (+) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63704.71 Da        Isoelectric Point: 4.4866

>NTDB_id=343925 EPB57_RS19545 WP_053307551.1 2402089..2403834(+) (pilQ) [Vibrio alginolyticus strain 2013V-1302]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYKHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=343925 EPB57_RS19545 WP_053307551.1 2402089..2403834(+) (pilQ) [Vibrio alginolyticus strain 2013V-1302]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCAGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACAAGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCTAATGAGAAAGCTCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTTGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTCAATGTAAACTTAGCTGCAACCAGTCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCAGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCGGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGACCTGCCGCTATTAGGTGCTCTTTTTCGTCGA
AGTTATGAGAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.943

100

0.929
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.861

99.312

0.396
  pilQ Legionella pneumophila strain Lp02

40.395

95.869

0.387
  pilQ Legionella pneumophila strain ERS1305867

40.395

95.869

0.387
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii D1279779

35.906

100

0.368
  pilQ Acinetobacter baumannii strain A118

35.906

100

0.368

Multiple sequence alignment