Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EPB57_RS19535 Genome accession   NZ_CP035700
Coordinates   2400965..2401558 (+) Length   197 a.a.
NCBI ID   WP_064354357.1    Uniprot ID   A0AAW7A2J1
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395965..2406558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS19520 - 2396691..2399252 (-) 2562 WP_005381325.1 PBP1A family penicillin-binding protein -
  EPB57_RS19525 pilM 2399397..2400413 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  EPB57_RS19530 pilN 2400397..2400972 (+) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EPB57_RS19535 pilO 2400965..2401558 (+) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB57_RS19540 pilP 2401548..2402063 (+) 516 WP_151514393.1 pilus assembly protein PilP Machinery gene
  EPB57_RS19545 pilQ 2402089..2403834 (+) 1746 WP_053307551.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB57_RS19550 aroK 2404022..2404540 (+) 519 WP_005381319.1 shikimate kinase AroK -
  EPB57_RS19555 aroB 2404573..2405673 (+) 1101 WP_005382764.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0885

>NTDB_id=343923 EPB57_RS19535 WP_064354357.1 2400965..2401558(+) (pilO) [Vibrio alginolyticus strain 2013V-1302]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=343923 EPB57_RS19535 WP_064354357.1 2400965..2401558(+) (pilO) [Vibrio alginolyticus strain 2013V-1302]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTGCTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGTGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACTCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.842

96.447

0.838
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68

Multiple sequence alignment