Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB57_RS19525 Genome accession   NZ_CP035700
Coordinates   2399397..2400413 (+) Length   338 a.a.
NCBI ID   WP_017821733.1    Uniprot ID   A0AAW7A029
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2394397..2405413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS19510 - 2394828..2395556 (-) 729 WP_005382781.1 redoxin family protein -
  EPB57_RS19515 oxyR 2395726..2396637 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  EPB57_RS19520 - 2396691..2399252 (-) 2562 WP_005381325.1 PBP1A family penicillin-binding protein -
  EPB57_RS19525 pilM 2399397..2400413 (+) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  EPB57_RS19530 pilN 2400397..2400972 (+) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EPB57_RS19535 pilO 2400965..2401558 (+) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB57_RS19540 pilP 2401548..2402063 (+) 516 WP_151514393.1 pilus assembly protein PilP Machinery gene
  EPB57_RS19545 pilQ 2402089..2403834 (+) 1746 WP_053307551.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB57_RS19550 aroK 2404022..2404540 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37595.95 Da        Isoelectric Point: 7.2224

>NTDB_id=343921 EPB57_RS19525 WP_017821733.1 2399397..2400413(+) (pilM) [Vibrio alginolyticus strain 2013V-1302]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIIERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343921 EPB57_RS19525 WP_017821733.1 2399397..2400413(+) (pilM) [Vibrio alginolyticus strain 2013V-1302]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCATAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment