Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EPB61_RS12335 Genome accession   NZ_CP035696
Coordinates   1025787..1027511 (-) Length   574 a.a.
NCBI ID   WP_004728568.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2419-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020787..1032511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB61_RS12315 - 1021477..1022307 (-) 831 WP_055465832.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EPB61_RS12320 - 1022402..1023922 (-) 1521 WP_154180459.1 AAA family ATPase -
  EPB61_RS12325 aroB 1023968..1025053 (-) 1086 WP_154180460.1 3-dehydroquinate synthase -
  EPB61_RS12330 aroK 1025081..1025599 (-) 519 WP_004728569.1 shikimate kinase AroK -
  EPB61_RS12335 pilQ 1025787..1027511 (-) 1725 WP_004728568.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB61_RS12340 pilP 1027536..1028048 (-) 513 WP_154180461.1 pilus assembly protein PilP Machinery gene
  EPB61_RS12345 pilO 1028038..1028628 (-) 591 WP_154180462.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB61_RS12350 pilN 1028621..1029211 (-) 591 WP_055465837.1 PilN domain-containing protein Machinery gene
  EPB61_RS12355 pilM 1029201..1030205 (-) 1005 WP_154180463.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61928.62 Da        Isoelectric Point: 4.4610

>NTDB_id=343818 EPB61_RS12335 WP_004728568.1 1025787..1027511(-) (pilQ) [Vibrio furnissii strain 2419-04]
MIKGLNASTAWGIRCLFVGVCLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLTIELLKT
DVSDDKLYLVDVRDFATAVENIEVFRQQPSTVLVATVQGAFNYEYSLKGNYLEVVISKVSAQEPQPKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNQFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDRREQQQLEK
ARMAEELGDLQSEIIKINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLLRELPDNIHVIRDIVASLDIPVKQVQ
IEARIVTVNEGDLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGSSDSGDGAAIDDFLNVNLAATSANASSIAFQVA
KLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLV
LDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVTSSVDKVPLLGDLPLLGALFRRSYEQMGK
SELLIFVTPKVVIQ

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=343818 EPB61_RS12335 WP_004728568.1 1025787..1027511(-) (pilQ) [Vibrio furnissii strain 2419-04]
ATGATTAAAGGACTAAATGCCTCCACGGCTTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACATTAGCCCAGGAAGCGGCGGCCAATCAGCTGAAAAATATCGACTTTCGTATCAACAAAGACAAAGCGGCGGTGTTGA
TTGTCGAACTGGCGTCGGCTTCTGCGGTGGTGGATGTGCAGAAAACGGAGCAAGGATTGACGATCGAATTGCTTAAAACC
GATGTTAGCGATGACAAACTGTATTTGGTGGACGTGCGCGATTTTGCCACGGCGGTGGAAAACATCGAAGTGTTTCGCCA
GCAACCGAGCACGGTGCTGGTGGCGACGGTGCAAGGGGCATTCAATTACGAATACAGCCTGAAAGGCAATTACTTGGAGG
TGGTGATCAGCAAAGTCAGTGCGCAAGAACCGCAACCGAAAAGTGTCTTAGAAAAAGAGGGCAAGCTGATTTCGATTAAC
TTCCAAGACATTCCCGTGCGCAACGTGCTGCAGCTCATCGCCGACTACAACCAGTTCAACTTGGTGGTCTCCGATTCCGT
CAGCGGCAACTTGACATTGCGCCTCGATGGCGTGCCGTGGCAGCAAGTACTGGATATCATTTTACAAGTCAAAGGGCTGG
ACAAACGGGTCGATGGCAACGTCATTCTGGTGGCGCCCAAAGAGGAGCTGGACCGACGCGAGCAGCAGCAATTGGAAAAA
GCGCGCATGGCGGAAGAGCTGGGCGATCTGCAATCGGAAATCATCAAAATCAATTTCGCCAAGGCATCTGACATTGCACA
GATGATCGGTGGGGATGGCGCGGTGAACATGTTGTCAGAGCGCGGCTCCATCAGTATTGATGAGCGCACCAACGCCTTGC
TACTGCGCGAGCTGCCTGACAACATTCACGTGATCCGCGACATCGTGGCCTCTCTCGACATTCCGGTCAAACAAGTGCAA
ATCGAAGCGCGCATCGTCACGGTGAATGAGGGCGATTTGGATGAGCTTGGCGTGCGCTGGGGCTTTAGTTCCACCAACGG
GAGCACCACGACCGGGGGCTCGATTGAAAGTAATCTCGGTGCCATTGGGTTGTATGACGGCAGCAGTGATTCGGGTGATG
GCGCCGCGATTGATGATTTCCTCAATGTGAACCTTGCTGCTACCTCCGCCAATGCATCCAGTATTGCGTTTCAAGTCGCC
AAGCTGGGCGCTGACACCTTACTTGATCTTGAATTGTCGGCGATGCAGCGCGAGTCGAAAGCGGAAATTATTTCCAGCCC
AAGGCTGATCACCACCAACAAACAGCCGGCTTACATTGAACAGGGGACGGAAATCCCTTATCTCGAATCGTCCTCCAGTG
GCGCAACCAGCGTGGCATTTAAAAAAGCGGTCCTCAGCCTCAAAGTGACCCCGCAAATCACTCCTGACAACCGTTTGGTG
CTCGATTTGAGCGTGACCCAAGACCGACCGGGTGAAGTGGTGAAAACGGGCACGGGCGAAGCGGTGGCGATTAACACGCA
GCGTATTGGGACCCAAGTGTTAGTTAATAACGGTGAAACCGTGGTGCTGGGCGGCATTTTTCAACACAGCGTGACCAGTT
CTGTGGACAAAGTGCCCTTGCTTGGGGACTTGCCATTACTGGGCGCGCTGTTCCGTCGCAGCTACGAACAGATGGGGAAA
AGTGAGCTACTGATCTTCGTCACGCCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Secretin and TonB N terminus short domain (STN)

(179-227)

Bacterial type II and III secretion system protein (Secretin)

(414-572)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

80.556

100

0.808
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.556

100

0.808
  pilQ Vibrio campbellii strain DS40M4

77.413

95.645

0.74
  pilQ Legionella pneumophila strain ERS1305867

41.575

95.122

0.395
  pilQ Legionella pneumophila strain Lp02

41.575

95.122

0.395
  comQ Acinetobacter baylyi ADP1

38.737

100

0.395
  pilQ Pseudomonas aeruginosa PAK

40.468

96.864

0.392
  pilQ Acinetobacter baumannii D1279779

37.113

100

0.376
  pilQ Acinetobacter baumannii strain A118

37.113

100

0.376

Multiple sequence alignment