Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB61_RS12355 Genome accession   NZ_CP035696
Coordinates   1029201..1030205 (-) Length   334 a.a.
NCBI ID   WP_154180463.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2419-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024201..1035205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB61_RS12330 aroK 1025081..1025599 (-) 519 WP_004728569.1 shikimate kinase AroK -
  EPB61_RS12335 pilQ 1025787..1027511 (-) 1725 WP_004728568.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB61_RS12340 pilP 1027536..1028048 (-) 513 WP_154180461.1 pilus assembly protein PilP Machinery gene
  EPB61_RS12345 pilO 1028038..1028628 (-) 591 WP_154180462.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB61_RS12350 pilN 1028621..1029211 (-) 591 WP_055465837.1 PilN domain-containing protein Machinery gene
  EPB61_RS12355 pilM 1029201..1030205 (-) 1005 WP_154180463.1 type IV pilus assembly protein PilM Machinery gene
  EPB61_RS12360 - 1030350..1032830 (+) 2481 WP_143680273.1 PBP1A family penicillin-binding protein -
  EPB61_RS12365 oxyR 1032917..1033813 (-) 897 WP_014204332.1 DNA-binding transcriptional regulator OxyR -
  EPB61_RS12370 - 1033957..1034691 (+) 735 WP_004728561.1 glutathione peroxidase -
  EPB61_RS12375 - 1034815..1035078 (+) 264 WP_004728560.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36864.16 Da        Isoelectric Point: 6.6617

>NTDB_id=343822 EPB61_RS12355 WP_154180463.1 1029201..1030205(-) (pilM) [Vibrio furnissii strain 2419-04]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSDDIFADNHTLDYQKIVKKLKELKKGLPLFSRKVALAVPDTA
VISKVLQIDSDLDASEQEFAVYQAFSHQSPFPIDDLSLDFVPSGDNPAGRSTNTLYQVYATRREVVDNRLSVASKAGFEP
LLLDVQVHGLLQIWQWVSRAQQRDDWMLVDVGLTQTTLCMDFADKAPFYKDIPLGTRQWHADPTPHPVPLSEPPMAGVNE
LVERIARQVQLLLSLQGHQALGGIWLSGGGATTEGLSAALAARLNLPCELFHPLTGFVTRRRMRARVPDDLQRFSTAAGL
ALRGLNWLERDHGA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=343822 EPB61_RS12355 WP_154180463.1 1029201..1030205(-) (pilM) [Vibrio furnissii strain 2419-04]
ATGGGTAAATCATTAGTTACAGGTGTCGATATCGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAGGGCGAATC
GTATTCGCTGGTCGGCTACCAAGAAATAGTCGTATCCGACGATATTTTCGCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGTTATTTAGTCGCAAAGTTGCGCTGGCTGTGCCGGATACTGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGATTTAGATGCCAGCGAACAAGAATTTGCTGTCTATCAGGCGTTTTCCCA
TCAATCGCCGTTTCCCATCGATGATCTGAGTTTGGATTTCGTACCAAGCGGCGATAACCCCGCCGGGCGCAGCACCAACA
CCCTGTATCAAGTGTACGCCACGCGACGCGAAGTCGTCGATAACCGGTTATCGGTGGCGAGCAAAGCGGGCTTTGAGCCG
CTGCTGCTCGATGTGCAGGTGCATGGGTTGCTCCAGATTTGGCAATGGGTGAGTCGCGCGCAGCAGCGAGACGATTGGAT
GCTGGTGGACGTCGGGCTGACGCAAACCACGTTATGCATGGATTTCGCCGATAAAGCGCCCTTCTATAAAGACATTCCGC
TGGGAACGCGTCAATGGCATGCCGACCCCACGCCCCATCCTGTGCCGCTGAGCGAGCCACCGATGGCTGGAGTCAACGAG
CTGGTTGAGCGAATTGCGCGCCAAGTGCAACTGCTGTTGTCCTTGCAGGGCCATCAAGCGCTGGGCGGGATTTGGCTGTC
GGGCGGTGGGGCGACGACTGAAGGCCTCAGCGCGGCTTTGGCTGCGCGACTGAATCTGCCGTGTGAACTGTTTCATCCGC
TGACGGGATTTGTGACGCGCCGCCGCATGCGAGCGCGTGTGCCAGACGATCTGCAACGTTTTAGTACGGCGGCCGGCTTG
GCGCTGCGCGGACTCAACTGGTTGGAGCGTGACCATGGTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(239-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.307

100

0.653
  pilM Vibrio campbellii strain DS40M4

44.97

100

0.455

Multiple sequence alignment