Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EPB61_RS12345 Genome accession   NZ_CP035696
Coordinates   1028038..1028628 (-) Length   196 a.a.
NCBI ID   WP_154180462.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2419-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1023038..1033628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB61_RS12325 aroB 1023968..1025053 (-) 1086 WP_154180460.1 3-dehydroquinate synthase -
  EPB61_RS12330 aroK 1025081..1025599 (-) 519 WP_004728569.1 shikimate kinase AroK -
  EPB61_RS12335 pilQ 1025787..1027511 (-) 1725 WP_004728568.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB61_RS12340 pilP 1027536..1028048 (-) 513 WP_154180461.1 pilus assembly protein PilP Machinery gene
  EPB61_RS12345 pilO 1028038..1028628 (-) 591 WP_154180462.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB61_RS12350 pilN 1028621..1029211 (-) 591 WP_055465837.1 PilN domain-containing protein Machinery gene
  EPB61_RS12355 pilM 1029201..1030205 (-) 1005 WP_154180463.1 type IV pilus assembly protein PilM Machinery gene
  EPB61_RS12360 - 1030350..1032830 (+) 2481 WP_143680273.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22658.91 Da        Isoelectric Point: 5.2955

>NTDB_id=343820 EPB61_RS12345 WP_154180462.1 1028038..1028628(-) (pilO) [Vibrio furnissii strain 2419-04]
MANVHDLELDEISEWPLVAQLIALVLLVLVLQGAGYWFYLQPKQDTLAAHKQQEQTLKSTIRIKASKVATLPKLKIQLDE
LAQRYDFLLRQLPVQKELASMLASVNELGLDNSLTFTRIDWGEKQNQQFLYRLPLNIELTGNYHDIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPGVSDEQ

Nucleotide


Download         Length: 591 bp        

>NTDB_id=343820 EPB61_RS12345 WP_154180462.1 1028038..1028628(-) (pilO) [Vibrio furnissii strain 2419-04]
ATGGCTAACGTACACGATCTGGAACTGGACGAAATCAGTGAATGGCCGTTGGTGGCTCAATTGATCGCCTTGGTGCTGCT
GGTGTTGGTGTTGCAGGGCGCGGGGTACTGGTTTTATTTGCAGCCTAAACAAGACACATTGGCCGCACACAAGCAGCAAG
AGCAAACGCTGAAGTCGACGATTCGCATCAAAGCCAGCAAAGTCGCGACATTGCCGAAGCTCAAGATTCAGCTGGATGAA
CTGGCACAGCGGTACGACTTCCTGTTGCGTCAGCTACCGGTGCAAAAAGAGCTCGCCAGCATGCTGGCGTCGGTGAACGA
GTTGGGGCTCGACAACAGCCTGACTTTTACTCGCATCGATTGGGGGGAAAAGCAGAATCAGCAATTTCTCTATCGGCTGC
CGCTCAATATCGAATTGACCGGTAATTATCACGACATCGGCGATTTTTCTGAAGCGATCGCGCGTCTACCGCGCATCATT
AATTTTGACGACGTTGACTGGCAGCGCGTCAGCCAAGAGAGCAGCACGCTCCATTTCCGCGTTCGCGCCTATACCTATCA
GTTCAAACCGGGGGTCAGTGATGAACAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

77.041

100

0.77
  pilO Vibrio campbellii strain DS40M4

70

96.939

0.679

Multiple sequence alignment