Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ET951_RS06855 Genome accession   NZ_CP035682
Coordinates   1466434..1468149 (+) Length   571 a.a.
NCBI ID   WP_187377498.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1461434..1473149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS06835 pilM 1463727..1464743 (+) 1017 WP_070381278.1 type IV pilus assembly protein PilM Machinery gene
  ET951_RS06840 pilN 1464733..1465317 (+) 585 WP_000903041.1 PilN domain-containing protein Machinery gene
  ET951_RS06845 pilO 1465310..1465900 (+) 591 WP_000135849.1 type 4a pilus biogenesis protein PilO Machinery gene
  ET951_RS06850 pilP 1465890..1466408 (+) 519 WP_154113182.1 pilus assembly protein PilP Machinery gene
  ET951_RS06855 pilQ 1466434..1468149 (+) 1716 WP_187377498.1 type IV pilus secretin PilQ Machinery gene
  ET951_RS06860 aroK 1468336..1468860 (+) 525 WP_000818614.1 shikimate kinase AroK -
  ET951_RS06865 aroB 1468888..1469973 (+) 1086 WP_192927868.1 3-dehydroquinate synthase -
  ET951_RS06870 - 1470027..1471391 (+) 1365 Protein_1272 AAA family ATPase -
  ET951_RS06875 - 1471566..1472399 (+) 834 WP_005524367.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62367.38 Da        Isoelectric Point: 4.5760

>NTDB_id=343604 ET951_RS06855 WP_187377498.1 1466434..1468149(+) (pilQ) [Vibrio mimicus strain 2011V-1073]
MRNGLKTYVAQTWLTLWAGLALCASSVAISAESASANQLENIDFRVNKDKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSVEVFRKEPSTQLIATIEGEFKHDYTLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARMAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGTISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLAEDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=343604 ET951_RS06855 WP_187377498.1 1466434..1468149(+) (pilQ) [Vibrio mimicus strain 2011V-1073]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGCTGGCTTGGCACTTTGTGCTTCTTCCGT
GGCGATTAGCGCTGAATCAGCTTCCGCCAATCAACTAGAAAACATTGATTTTCGGGTCAATAAAGACAAAGCGGCTGTGT
TGATTGTCGAATTGGCTTCGCCATCAGCGGTGGTGGATGTGCAAAAAGTCCAAGAAGGGCTGAGTATTGAACTCCTGAAA
ACCGATGTGGCAGACGACAAACTCTATTTATTGGATGTGAAGGATTTCTCTACACCAGTACAAAGCGTAGAAGTCTTCCG
TAAAGAACCCAGTACCCAGCTTATCGCAACCATCGAAGGTGAGTTTAAGCATGACTACACCTTAAAAGGGAAATACCTTG
AGGTGGTAATCAGTAAGCTCAAAGCAGACGAGAAACCTCAGCCTAAAAGTATTTTGGAAAAAGAGGGCAAGCTGATCTCG
ATTAACTTCCAAGATATTCCAGTACGTAACGTACTGCAGTTGATTGCAGATTATAACGGTTTCAACCTCGTTGTTTCGGA
TTCGGTCTCGGGCAATCTGACTTTGCGTTTGGATGGTGTACCTTGGCAGCAAGTGCTGGATATTATCCTGCAGGTTAAAG
GGCTTGATAAACGCGTTGATGGCAATGTAATCCTGATTGCGCCAAAAGAAGAGCTGGATTTGCGTGAGAAACAAGCGTTA
GAAAAAGCACGTATGGCAGAAGAATTAGGTGATTTAAAATCTGAAATCATCAAAATTAACTTTGCCAAAGCATCTGATAT
CGCAGCAATGATTGGTGGTGAAGGTAACGTCAATATGCTGTCTGAGCGTGGCACGATCAGTATTGATGAGCGTACTAACT
CACTGCTGATTCGTGAATTGCCCGATAATATCGCGGTGATCCGTGAAATCATCGAATCGCTAGATATTCCGGTCAAGCAA
GTTCAGATTGAAGCACGTATTGTCACCGTAAAAGAAGGTAACTTGGATGAGCTTGGTGTACGTTGGGGTGTTATGTCAAC
CAACGGCAGCCACTCTGTGGGTGGAACCATTGAAAGTAACTTATGGCAACAAGGACTGCTGGCGGAAGATGAGTTTCCAG
TTGATGAGTTTTTGAATGTTAACTTGGCCTCTACGTCCGCTAATGCCTCTTCTATCGCTTTCCAAGTTGCTAAGTTGGGC
TCAGATACCTTGTTGGATTTGGAGCTATCTGCGTTACAAAATGAATCGAAGGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACGACCAATAAACAGCCTGCATATATTGAACAAGGTACTGAAATTCCTTATCTCGAATCTTCATCTAGTGGTGCAACAA
CCGTATCGTTCAAGAAAGCCGTGTTGAGCTTGAAAGTGACACCACAAATTACGCCGGATAATCGTTTAGTACTGGATTTG
AGTGTGACTCAGGACCGTCGTGGCGAAACGGTGAAAACAGGAACAGGTGAAGCGGTATCGATCGATACCCAGCGTATTGG
TACACAAGTTCTGGTCAATAATGGTGAAACCGTCGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCAGTGGATA
AGGTGCCGTTGCTAGGTGATCTCCCTGTTTTAGGTGCGTTGTTTAGACGAACTTACGAACAAATGGGCAAAAGTGAGCTG
CTGATTTTCGTCACACCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(181-229)

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.622

100

0.956
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.622

100

0.956
  pilQ Vibrio campbellii strain DS40M4

78.584

96.497

0.758
  pilQ Legionella pneumophila strain Lp02

43.173

94.921

0.41
  pilQ Legionella pneumophila strain ERS1305867

43.173

94.921

0.41
  comQ Acinetobacter baylyi ADP1

39.446

100

0.399
  pilQ Acinetobacter baumannii D1279779

38.33

100

0.394
  pilQ Acinetobacter baumannii strain A118

38.33

100

0.394
  pilQ Pseudomonas aeruginosa PAK

39.785

97.723

0.389

Multiple sequence alignment