Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EWA66_RS02300 Genome accession   NZ_CP035680
Coordinates   491231..493018 (-) Length   595 a.a.
NCBI ID   WP_039438954.1    Uniprot ID   -
Organism   Vibrio navarrensis strain 08-2462     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486231..498018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA66_RS02280 - 486916..487743 (-) 828 WP_039438962.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EWA66_RS02285 - 487823..489328 (-) 1506 WP_039438959.1 AAA family ATPase -
  EWA66_RS02290 aroB 489380..490470 (-) 1091 Protein_419 3-dehydroquinate synthase -
  EWA66_RS02295 aroK 490503..491021 (-) 519 WP_039438955.1 shikimate kinase AroK -
  EWA66_RS02300 pilQ 491231..493018 (-) 1788 WP_039438954.1 type IV pilus secretin PilQ Machinery gene
  EWA66_RS02305 pilP 493037..493549 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA66_RS02310 pilO 493539..494129 (-) 591 WP_039438948.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA66_RS02315 pilN 494122..494709 (-) 588 WP_039423009.1 PilN domain-containing protein Machinery gene
  EWA66_RS02320 pilM 494693..495712 (-) 1020 WP_039438945.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 595 a.a.        Molecular weight: 64492.65 Da        Isoelectric Point: 4.7109

>NTDB_id=343535 EWA66_RS02300 WP_039438954.1 491231..493018(-) (pilQ) [Vibrio navarrensis strain 08-2462]
MIREMTMKKGMIRLQAPLCQLMCLFAVCLFAAFANAQPENNSQSASENALKSIDFRTNQNKAAVIIVELASPAAVVDLKP
VQEGLSIELLNTRVSDEQLYLLDVKDFSTPVESVEVFRDVSATRLLASVSGEFRHEYRLTGRYLEVTVSRIETPQQAEEK
SILEKEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPK
EELDLREKQHLEKQRLAEELGTLKSEIIKVNFAKASDIAEMISGTESVSMLSERGSIAIDERTNSLLLRELPENIAVIRE
IVNSLDIPVKQVQIEARIVTVNEGNLDELGVRWGFTSVNGSHTVGGSIENNLASVGLYNAAGGGSGSGGDSGGGSSSIDD
FLNVNLGATSGNASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVS
FKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVTSTVDKVP
LLGDLPLLGALFRRSYENIGKRELLIFVTPKVVIQ

Nucleotide


Download         Length: 1788 bp        

>NTDB_id=343535 EWA66_RS02300 WP_039438954.1 491231..493018(-) (pilQ) [Vibrio navarrensis strain 08-2462]
GTGATTAGAGAAATGACCATGAAGAAAGGAATGATTCGATTACAGGCTCCGCTATGCCAATTAATGTGCCTGTTTGCTGT
CTGCCTCTTTGCCGCGTTTGCAAACGCGCAGCCAGAGAATAACAGTCAGTCAGCGAGTGAGAATGCGCTGAAAAGCATTG
ACTTTAGAACCAACCAGAATAAAGCCGCGGTGATCATTGTCGAGTTGGCTTCGCCTGCCGCGGTGGTGGATCTTAAACCT
GTGCAGGAGGGGCTTAGCATTGAGCTGCTCAACACCCGCGTCAGCGACGAACAGCTCTATTTACTAGACGTTAAAGACTT
CTCGACGCCAGTTGAAAGTGTTGAAGTGTTCCGTGATGTGTCAGCGACGCGTTTGCTGGCGTCTGTCTCGGGGGAGTTTC
GCCATGAATATCGTTTGACTGGGCGCTATTTGGAAGTGACGGTCAGTCGCATAGAAACCCCGCAGCAGGCCGAGGAGAAA
AGCATCCTAGAAAAAGAGGGCAAGCTTATCTCGATCAACTTCCAAGACATTCCGGTGAGGAACGTGTTGCAACTGATCGC
GGACTACAACGGTTTTAACCTAGTGGTCTCCGATTCCGTCACGGGTAATCTGACCTTGCGTTTGGATGGTGTGCCGTGGC
AGCAAGTGCTCGACATCATTTTGCAAGTCAAAGGGCTCGACAAGCGGGTGGAAGGCAATGTGGTGCTGATCGCGCCAAAA
GAAGAGTTAGATTTGCGTGAGAAGCAGCATCTGGAAAAGCAGCGCTTGGCGGAAGAGTTAGGCACACTCAAATCGGAAAT
CATCAAGGTGAACTTTGCCAAAGCGTCCGACATTGCCGAGATGATCAGCGGCACGGAGTCGGTCAGCATGCTGTCCGAGC
GCGGCAGCATCGCGATTGATGAACGCACAAACTCCTTGCTACTACGAGAACTGCCGGAAAACATTGCGGTGATTCGGGAG
ATTGTCAATTCGCTCGATATTCCGGTCAAACAGGTGCAAATTGAAGCACGCATTGTCACCGTTAATGAAGGCAATTTGGA
TGAATTGGGCGTGCGTTGGGGCTTCACCTCGGTCAACGGCAGCCACACGGTGGGTGGTTCGATCGAGAACAACTTGGCGA
GCGTCGGTTTGTACAACGCGGCTGGTGGTGGTTCTGGCTCAGGAGGCGATAGCGGTGGTGGATCCAGCAGTATCGACGAT
TTTCTCAACGTCAATCTGGGGGCGACCTCAGGCAATGCATCAAGTATCGCTTTCCAAGTGGCCAAGCTGGGCTCGGACCT
GCTGCTCGACCTAGAGCTGTCGGCGCTACAGCGAGAGTCAAAAGCGGAGATCATTTCCAGCCCGCGTCTGATTACCACCA
ATAAAAAGCCGGCCTACATCGAACAAGGGACCGAAATTCCCTATCTGGAATCGTCCTCCAGTGGCGCGACTACCGTTTCG
TTCAAAAAAGCGGTACTGAGTTTGAAAGTCACGCCGCAGATCACCCCGGATAACCGTCTGGTGCTCGATCTTAGCGTGAC
ACAAGACAGGCCGGGTGAAGTGGTGAAAACCGGTACGGGTGAAGCGGTGGCGATTGATACCCAACGTATCGGCACTCAGG
TGTTGGTTAACAATGGTGAAACCGTGGTTCTCGGCGGCATTTTCCAACATAGCGTCACCAGTACGGTCGACAAAGTGCCT
TTGCTGGGTGACCTGCCACTGCTTGGCGCGCTGTTTCGCCGCAGCTATGAAAATATCGGTAAACGGGAACTGTTGATCTT
TGTGACGCCAAAGGTGGTTATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(266-331)

Bacterial type II and III secretion system protein (Secretin)

(435-593)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

78.342

99.328

0.778
  pilQ Vibrio cholerae strain A1552

78.559

93.277

0.733
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.559

93.277

0.733
  pilQ Pseudomonas aeruginosa PAK

40.575

93.613

0.38
  pilQ Legionella pneumophila strain ERS1305867

40.647

93.445

0.38
  pilQ Legionella pneumophila strain Lp02

40.647

93.445

0.38
  comQ Acinetobacter baylyi ADP1

37.966

99.16

0.376
  pilQ Acinetobacter baumannii strain A118

36.486

99.496

0.363
  pilQ Acinetobacter baumannii D1279779

36.486

99.496

0.363

Multiple sequence alignment