Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EWA66_RS02310 Genome accession   NZ_CP035680
Coordinates   493539..494129 (-) Length   196 a.a.
NCBI ID   WP_039438948.1    Uniprot ID   -
Organism   Vibrio navarrensis strain 08-2462     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488539..499129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA66_RS02290 aroB 489380..490470 (-) 1091 Protein_419 3-dehydroquinate synthase -
  EWA66_RS02295 aroK 490503..491021 (-) 519 WP_039438955.1 shikimate kinase AroK -
  EWA66_RS02300 pilQ 491231..493018 (-) 1788 WP_039438954.1 type IV pilus secretin PilQ Machinery gene
  EWA66_RS02305 pilP 493037..493549 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA66_RS02310 pilO 493539..494129 (-) 591 WP_039438948.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA66_RS02315 pilN 494122..494709 (-) 588 WP_039423009.1 PilN domain-containing protein Machinery gene
  EWA66_RS02320 pilM 494693..495712 (-) 1020 WP_039438945.1 type IV pilus biogenesis protein PilM Machinery gene
  EWA66_RS02325 - 495851..498367 (+) 2517 WP_154199794.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22757.01 Da        Isoelectric Point: 4.3163

>NTDB_id=343537 EWA66_RS02310 WP_039438948.1 493539..494129(-) (pilO) [Vibrio navarrensis strain 08-2462]
MVDWQDLDIDEIAEWPLLPQSLVVLLLMMIIQGFSYWFYLQPEQEALQRAVEEEKNVKIALRIKANKAAALPQLQSQLDE
LSNRYEYLLQQLPAQKELATLLASVNELGLENDLTFTRIDWGEKLSKDFLYQLPLNIELTGDYNDIGQFSQAIAELPRII
SFSDVTWQRVSLESSTLHFRVKANTYQFKPEVPHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=343537 EWA66_RS02310 WP_039438948.1 493539..494129(-) (pilO) [Vibrio navarrensis strain 08-2462]
ATGGTTGATTGGCAGGATCTCGATATCGACGAAATAGCGGAGTGGCCATTATTGCCACAGAGCTTAGTGGTGCTGCTCCT
GATGATGATCATTCAAGGCTTTAGCTACTGGTTCTATCTGCAACCTGAGCAAGAGGCGTTGCAAAGGGCGGTGGAGGAAG
AGAAAAACGTCAAAATCGCGCTGCGTATCAAAGCCAACAAAGCGGCTGCCTTGCCACAGCTTCAGTCTCAGTTGGATGAG
TTATCAAACCGTTACGAGTATTTGCTCCAGCAACTGCCTGCGCAAAAAGAGCTGGCGACCTTGCTCGCGTCGGTCAATGA
ACTCGGGCTGGAAAACGATCTCACCTTTACCCGCATCGATTGGGGGGAAAAACTGAGCAAGGATTTCCTCTATCAGCTTC
CTCTCAACATTGAACTGACTGGTGATTACAACGACATCGGTCAGTTTTCCCAAGCGATCGCCGAGCTGCCGCGCATCATT
AGCTTTAGCGACGTCACTTGGCAGCGGGTCAGTTTGGAGAGCAGCACACTGCATTTTCGCGTTAAAGCCAACACGTACCA
GTTTAAGCCGGAGGTGCCCCATGAAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

74.737

96.939

0.724
  pilO Vibrio cholerae strain A1552

67.857

100

0.679

Multiple sequence alignment