Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EWA66_RS02320 Genome accession   NZ_CP035680
Coordinates   494693..495712 (-) Length   339 a.a.
NCBI ID   WP_039438945.1    Uniprot ID   -
Organism   Vibrio navarrensis strain 08-2462     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489693..500712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA66_RS02295 aroK 490503..491021 (-) 519 WP_039438955.1 shikimate kinase AroK -
  EWA66_RS02300 pilQ 491231..493018 (-) 1788 WP_039438954.1 type IV pilus secretin PilQ Machinery gene
  EWA66_RS02305 pilP 493037..493549 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA66_RS02310 pilO 493539..494129 (-) 591 WP_039438948.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA66_RS02315 pilN 494122..494709 (-) 588 WP_039423009.1 PilN domain-containing protein Machinery gene
  EWA66_RS02320 pilM 494693..495712 (-) 1020 WP_039438945.1 type IV pilus biogenesis protein PilM Machinery gene
  EWA66_RS02325 - 495851..498367 (+) 2517 WP_154199794.1 penicillin-binding protein 1A -
  EWA66_RS02330 oxyR 498445..499338 (-) 894 WP_039440791.1 DNA-binding transcriptional regulator OxyR -
  EWA66_RS02335 - 499509..499766 (+) 258 WP_039422999.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38199.95 Da        Isoelectric Point: 7.3959

>NTDB_id=343539 EWA66_RS02320 WP_039438945.1 494693..495712(-) (pilM) [Vibrio navarrensis strain 08-2462]
MGKPLVTGIDIGHRSLKAVILKPAGNSYALLGYKEIVFERAIVAENHTLNHQEIVNTLKVLNKELPRFRRQVALAVPDSA
VISKQLQIEPIEDEKECEIAILQAFSHQSPFPVDELNIDYVRLHEPAQSKSASQSVQIFATKKEVVESRVRAAQAVRLKP
VLVDMHAHCLFNLRDLAAQRYPDKSAFCLLDVGAQTTSLIIHQREKTHFIKEFAYGVEHLNAAIGDEFSAHDAQEKTERF
IVEMVDRVARQLQLFQSINSQDKPQGLWLCGEGAATPLLLEALSRRLSLECELLNPLGLFEMKVARKRRRSVDWHHFSVA
AGLAIGGIQWLENKHEAHH

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=343539 EWA66_RS02320 WP_039438945.1 494693..495712(-) (pilM) [Vibrio navarrensis strain 08-2462]
ATGGGTAAACCCTTAGTTACCGGGATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCCGCAGGAAATAG
CTATGCCTTGCTGGGATATAAAGAAATTGTATTTGAGCGCGCTATTGTCGCCGAAAACCACACTCTGAATCATCAGGAAA
TTGTCAATACGCTTAAAGTCCTGAATAAAGAGTTGCCGCGTTTCCGTCGTCAGGTGGCGCTGGCCGTGCCGGATAGTGCC
GTGATCAGCAAGCAATTGCAAATTGAACCGATAGAAGATGAGAAAGAGTGTGAAATCGCCATTTTGCAGGCTTTTTCCCA
TCAATCGCCGTTTCCGGTCGATGAGCTAAATATTGATTATGTGCGTTTGCATGAACCCGCGCAGAGTAAAAGCGCCAGTC
AGAGCGTGCAGATCTTTGCCACTAAGAAAGAGGTGGTCGAGAGTCGGGTGCGCGCCGCGCAGGCGGTGAGGTTGAAACCT
GTGTTGGTGGACATGCACGCCCACTGCTTGTTTAACCTGCGAGATCTGGCGGCGCAGCGCTATCCAGACAAAAGTGCCTT
TTGCCTGTTGGATGTGGGCGCGCAAACCACCTCACTGATAATTCATCAGCGCGAGAAAACGCACTTTATCAAAGAGTTCG
CGTACGGCGTTGAGCACTTGAACGCCGCGATTGGCGATGAGTTCAGTGCCCATGATGCGCAGGAGAAGACCGAGCGCTTT
ATTGTGGAGATGGTGGATCGCGTCGCGCGACAGTTACAACTCTTTCAGTCCATCAATAGTCAAGACAAGCCACAAGGGCT
TTGGTTGTGCGGCGAGGGAGCGGCGACGCCCCTGTTGCTCGAGGCGCTCTCTCGTCGTTTGTCGTTGGAGTGTGAACTGC
TCAATCCGCTCGGTCTGTTTGAGATGAAAGTGGCACGCAAAAGACGTCGCAGTGTTGACTGGCACCACTTTTCGGTCGCC
GCTGGGTTAGCCATCGGTGGGATTCAATGGTTGGAGAACAAACATGAAGCACACCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

54.572

100

0.546
  pilM Vibrio cholerae strain A1552

47.246

100

0.481

Multiple sequence alignment