Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F4V39_RS13530 Genome accession   NZ_CP044148
Coordinates   2577698..2579248 (-) Length   516 a.a.
NCBI ID   WP_000275014.1    Uniprot ID   -
Organism   Escherichia coli O157 strain AR-0427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572698..2584248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V39_RS13505 ataR 2573087..2573353 (-) 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR -
  F4V39_RS13510 livJ 2573503..2574606 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  F4V39_RS13515 rpoH 2574877..2575731 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  F4V39_RS13520 ftsX 2575976..2577034 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  F4V39_RS13525 ftsE 2577027..2577695 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  F4V39_RS13530 pilA 2577698..2579248 (-) 1551 WP_000275014.1 signal recognition particle-docking protein FtsY Machinery gene
  F4V39_RS13535 rsmD 2579343..2579939 (+) 597 WP_000743199.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  F4V39_RS13540 yhhL 2579929..2580198 (+) 270 WP_001295207.1 DUF1145 family protein -
  F4V39_RS13545 yhhM 2580201..2580560 (-) 360 WP_000042873.1 DUF2500 domain-containing protein -
  F4V39_RS13550 yhhN 2580701..2581327 (+) 627 WP_000964718.1 lysoplasmalogenase -
  F4V39_RS13555 zntA 2581401..2583599 (+) 2199 WP_000106562.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  F4V39_RS13560 tusA 2583701..2583946 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 56658.72 Da        Isoelectric Point: 4.1719

>NTDB_id=341149 F4V39_RS13530 WP_000275014.1 2577698..2579248(-) (pilA) [Escherichia coli O157 strain AR-0427]
MYIALTLCHEATARSVVANGERKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEA
EAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAE
EEAAKEEITDEELEAQALAAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGK
KIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGV
NGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIA
DTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVA
DQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=341149 F4V39_RS13530 WP_000275014.1 2577698..2579248(-) (pilA) [Escherichia coli O157 strain AR-0427]
ATGTACATAGCGTTAACGCTGTGCCATGAAGCAACAGCGAGGAGTGTAGTCGCAAATGGCGAAAGAAAAAAACGTGGCTT
TTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGAAGTTCAGAATGAACAAACGGTTGTAG
AAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAGAGCAGCCGCAGGCGCATACTGAGGCC
GAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAAAATGAAAAAGCGCAGCCTGAAGCGGA
AGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTATCGAACGTGAAGAGCTGCCGTTGCCGG
AAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAA
GAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTGGCTGCCGAAGCAGCAGAAGAGGCGGT
GATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTT
TTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGATTTATCAGCCTGTTCCGCGGTAAA
AAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGCGTGGAAACCACACGTAAAAT
TATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCTCTATGGCCTGCTGAAAGAAGAGA
TGGGTGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGTTCGTGATCCTGATGGTGGGCGTC
AACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAGCAGGGTAAATCGGTGATGCTGGCGGC
GGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCGCAACAATATTCCGGTGATTGCCCAGC
ATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCGGCTAAAGCGCGTAATATCGACGTCCTGATTGCC
GATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGA
CGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCAAACTGTTCC
ATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAGGCGGGGTAATTTTCTCGGTGGCT
GACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTGCGTCCGTTTAAGGCGGACGACTT
TATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(312-512)

SRP54-type protein, helical bundle domain (SRP54_N)

(229-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

74.612

0.364

Multiple sequence alignment