Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   EQH32_RS09660 Genome accession   NZ_CP035248
Coordinates   1868058..1868519 (-) Length   153 a.a.
NCBI ID   WP_000250538.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901939     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1863058..1873519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH32_RS09640 (EQH32_10150) comYH 1864286..1865239 (-) 954 WP_000345118.1 class I SAM-dependent methyltransferase Machinery gene
  EQH32_RS09645 (EQH32_10155) - 1865585..1866386 (+) 802 WP_128887635.1 IS5 family transposase -
  EQH32_RS09650 (EQH32_10160) - 1866538..1867014 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  EQH32_RS09655 (EQH32_10165) comGG/cglG 1867688..1868080 (-) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH32_RS09660 (EQH32_10170) comGF/cglF 1868058..1868519 (-) 462 WP_000250538.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH32_RS09665 (EQH32_10175) comGE/cglE 1868482..1868784 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH32_RS09670 (EQH32_10180) comGD/cglD 1868747..1869151 (-) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH32_RS09675 (EQH32_10185) comGC/cglC 1869144..1869470 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  EQH32_RS09680 (EQH32_10190) comGB/cglB 1869472..1870488 (-) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH32_RS09685 (EQH32_10195) comGA/cglA/cilD 1870436..1871377 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH32_RS09690 (EQH32_10200) - 1871453..1871818 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH32_RS09695 (EQH32_10205) - 1871970..1873028 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17763.28 Da        Isoelectric Point: 7.1668

>NTDB_id=337624 EQH32_RS09660 WP_000250538.1 1868058..1868519(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901939]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=337624 EQH32_RS09660 WP_000250538.1 1868058..1868519(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901939]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGGCCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae D39

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae R6

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae TIGR4

94.118

100

0.941
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915

Multiple sequence alignment