Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EQH41_RS09815 Genome accession   NZ_CP035239
Coordinates   1946435..1947376 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   Q8DN86
Organism   Streptococcus pneumoniae strain TVO_TIGR4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1941435..1952376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH41_RS09775 (EQH41_10405) comYH 1941921..1942874 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  EQH41_RS09780 (EQH41_10410) - 1942935..1943529 (-) 595 Protein_1933 class I SAM-dependent methyltransferase -
  EQH41_RS09785 (EQH41_10415) comGG/cglG 1943666..1944079 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH41_RS09790 (EQH41_10420) comGF/cglF 1944057..1944518 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH41_RS09795 (EQH41_10425) comGE/cglE 1944481..1944783 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH41_RS09800 (EQH41_10430) comGD/cglD 1944746..1945150 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH41_RS09805 (EQH41_10435) comGC/cglC 1945143..1945469 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  EQH41_RS09810 (EQH41_10440) comGB/cglB 1945471..1946487 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH41_RS09815 (EQH41_10445) comGA/cglA/cilD 1946435..1947376 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH41_RS09820 (EQH41_10450) - 1947452..1947817 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH41_RS09825 (EQH41_10455) - 1947968..1949026 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH41_RS09830 (EQH41_10460) nagA 1949189..1950340 (-) 1152 WP_001134456.1 N-acetylglucosamine-6-phosphate deacetylase -
  EQH41_RS09835 (EQH41_10465) - 1950493..1952310 (-) 1818 WP_001220853.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=337008 EQH41_RS09815 WP_000249564.1 1946435..1947376(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_TIGR4]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=337008 EQH41_RS09815 WP_000249564.1 1946435..1947376(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_TIGR4]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae R6

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment