Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   EQJ95_RS03750 Genome accession   NZ_CP035154
Coordinates   754774..755199 (+) Length   141 a.a.
NCBI ID   WP_128472132.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain SRCM103387     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 747060..774998 754774..755199 within 0


Gene organization within MGE regions


Location: 747060..774998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQJ95_RS03675 (EQJ95_03675) - 747060..747749 (-) 690 WP_008842223.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  EQJ95_RS03685 (EQJ95_03685) - 748145..749224 (-) 1080 WP_128472120.1 site-specific integrase -
  EQJ95_RS03690 (EQJ95_03690) - 749445..749642 (-) 198 WP_128472121.1 hypothetical protein -
  EQJ95_RS03695 (EQJ95_03695) - 749875..750642 (-) 768 WP_235020500.1 DUF4352 domain-containing protein -
  EQJ95_RS03700 (EQJ95_03700) - 750713..751120 (-) 408 WP_128472123.1 ImmA/IrrE family metallo-endopeptidase -
  EQJ95_RS03705 (EQJ95_03705) - 751132..751488 (-) 357 WP_164905797.1 helix-turn-helix transcriptional regulator -
  EQJ95_RS03710 (EQJ95_03710) - 751754..751942 (+) 189 WP_128472124.1 hypothetical protein -
  EQJ95_RS03715 (EQJ95_03715) - 751956..752159 (+) 204 WP_164905798.1 MW1434 family type I TA system toxin -
  EQJ95_RS03720 (EQJ95_03720) - 752220..752945 (+) 726 WP_128472126.1 Rha family transcriptional regulator -
  EQJ95_RS03725 (EQJ95_03725) - 753014..753220 (+) 207 WP_128472127.1 hypothetical protein -
  EQJ95_RS03730 (EQJ95_03730) - 753317..753634 (+) 318 WP_128472128.1 hypothetical protein -
  EQJ95_RS03735 (EQJ95_03735) - 753715..753927 (+) 213 WP_128472129.1 hypothetical protein -
  EQJ95_RS03740 (EQJ95_03740) - 753937..754572 (+) 636 WP_128472130.1 ERF family protein -
  EQJ95_RS03745 (EQJ95_03745) - 754575..754781 (+) 207 WP_128472131.1 hypothetical protein -
  EQJ95_RS03750 (EQJ95_03750) ssb 754774..755199 (+) 426 WP_128472132.1 single-stranded DNA-binding protein Machinery gene
  EQJ95_RS03755 (EQJ95_03755) - 755211..755888 (+) 678 WP_128472133.1 putative HNHc nuclease -
  EQJ95_RS03760 (EQJ95_03760) - 755878..756102 (+) 225 WP_128472134.1 hypothetical protein -
  EQJ95_RS03765 (EQJ95_03765) - 756106..756354 (-) 249 WP_128472135.1 hypothetical protein -
  EQJ95_RS03770 (EQJ95_03770) - 756388..757149 (+) 762 WP_128472136.1 conserved phage C-terminal domain-containing protein -
  EQJ95_RS03775 (EQJ95_03775) - 757130..757945 (+) 816 WP_128472137.1 ATP-binding protein -
  EQJ95_RS10310 - 758063..758440 (+) 378 WP_241648173.1 hypothetical protein -
  EQJ95_RS10315 - 758477..758695 (+) 219 WP_241648174.1 hypothetical protein -
  EQJ95_RS03785 (EQJ95_03785) - 758676..759077 (+) 402 WP_164905799.1 RusA family crossover junction endodeoxyribonuclease -
  EQJ95_RS10215 - 759074..759223 (+) 150 WP_164905800.1 hypothetical protein -
  EQJ95_RS03790 (EQJ95_03790) - 759210..759581 (+) 372 WP_128472139.1 N-acetylmuramoyl-L-alanine amidase -
  EQJ95_RS03795 (EQJ95_03795) - 759748..760041 (+) 294 WP_058121180.1 hypothetical protein -
  EQJ95_RS03800 (EQJ95_03800) - 760042..760287 (+) 246 WP_036672515.1 hypothetical protein -
  EQJ95_RS03805 (EQJ95_03805) - 760674..760922 (+) 249 WP_065124213.1 hypothetical protein -
  EQJ95_RS03810 (EQJ95_03810) - 761109..761297 (+) 189 WP_036672512.1 hypothetical protein -
  EQJ95_RS03815 (EQJ95_03815) - 761497..761940 (+) 444 WP_128472140.1 hypothetical protein -
  EQJ95_RS03820 (EQJ95_03820) - 762449..762700 (+) 252 WP_128472141.1 hypothetical protein -
  EQJ95_RS03825 (EQJ95_03825) - 762704..763216 (+) 513 WP_128472142.1 HNH endonuclease -
  EQJ95_RS03830 (EQJ95_03830) - 763314..763571 (+) 258 WP_128472143.1 hypothetical protein -
  EQJ95_RS03835 (EQJ95_03835) - 763762..764220 (+) 459 WP_128472144.1 phage terminase small subunit P27 family -
  EQJ95_RS03840 (EQJ95_03840) - 764223..766121 (+) 1899 WP_087116079.1 terminase TerL endonuclease subunit -
  EQJ95_RS03845 (EQJ95_03845) - 766111..766305 (+) 195 WP_128472145.1 DUF1056 family protein -
  EQJ95_RS03850 (EQJ95_03850) - 766308..767471 (+) 1164 WP_024863128.1 phage portal protein -
  EQJ95_RS03855 (EQJ95_03855) - 767449..768195 (+) 747 WP_128472146.1 head maturation protease, ClpP-related -
  EQJ95_RS03860 (EQJ95_03860) - 768195..769427 (+) 1233 WP_128472147.1 phage major capsid protein -
  EQJ95_RS03865 (EQJ95_03865) - 769500..769832 (+) 333 WP_002830407.1 head-tail connector protein -
  EQJ95_RS03870 (EQJ95_03870) - 769822..770169 (+) 348 WP_128472148.1 phage head closure protein -
  EQJ95_RS03875 (EQJ95_03875) - 770172..770579 (+) 408 WP_024863133.1 HK97-gp10 family putative phage morphogenesis protein -
  EQJ95_RS03880 (EQJ95_03880) - 770579..770959 (+) 381 WP_024863134.1 DUF806 family protein -
  EQJ95_RS03885 (EQJ95_03885) - 770973..771659 (+) 687 WP_024863135.1 phage tail protein -
  EQJ95_RS03890 (EQJ95_03890) - 771735..772109 (+) 375 WP_024863136.1 phage tail assembly chaperone -
  EQJ95_RS10420 - 772030..772254 (+) 225 WP_276330823.1 DUF2264 domain-containing protein -
  EQJ95_RS03895 (EQJ95_03895) - 772292..773698 (+) 1407 WP_276330824.1 DUF2264 domain-containing protein -
  EQJ95_RS03900 (EQJ95_03900) - 773733..774998 (+) 1266 WP_070366038.1 MFS transporter -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15628.32 Da        Isoelectric Point: 6.9816

>NTDB_id=334904 EQJ95_RS03750 WP_128472132.1 754774..755199(+) (ssb) [Pediococcus acidilactici strain SRCM103387]
MINRTVLIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFAKYTQKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNARQASTPGDPFANGGQSIDISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=334904 EQJ95_RS03750 WP_128472132.1 754774..755199(+) (ssb) [Pediococcus acidilactici strain SRCM103387]
ATGATTAATCGAACAGTACTAATAGGACGCCTAACTAAAGATGTTGAGCTTCGCCACACGGCTAAAGGCGATGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGCGAAGCGGATTTCATCAACTGTGTAATGT
GGCGTAAGGCAGCAGAAAACTTTGCCAAGTACACACAAAAAGGTTCGTTGGTAGGCATTGACGGTCGAATTCAAACCCGT
TCGTACGAAAACCAACAAGGACAACGAGTTTATGTAACTGAGGTTGTAGCTGATAACTTCTCGTTGCTAGATTCAAAACC
AAAAGGCAACCAGCAAAATAACGCACGGCAAGCATCAACGCCGGGAGATCCATTCGCCAATGGCGGACAGTCAATTGATA
TTAGTGACGACGATTTACCGTTCTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60

100

0.723
  ssbA Bacillus subtilis subsp. subtilis str. 168

55.233

100

0.674
  ssbB Bacillus subtilis subsp. subtilis str. 168

62.037

76.596

0.475
  ssbB/cilA Streptococcus mitis NCTC 12261

42.254

100

0.426
  ssbB/cilA Streptococcus pneumoniae TIGR4

42.254

100

0.426
  ssbB/cilA Streptococcus pneumoniae Rx1

41.549

100

0.418
  ssbB/cilA Streptococcus pneumoniae D39

41.549

100

0.418
  ssbB/cilA Streptococcus pneumoniae R6

41.549

100

0.418
  ssbB/cilA Streptococcus mitis SK321

41.549

100

0.418
  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.549

100

0.418
  ssbA Streptococcus mutans UA159

38.028

100

0.383

Multiple sequence alignment