Detailed information NTG0333003

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ELQ88_RS19945
• Genome accession: NZ_CP034783
• Coordinates: 3884008..3885117 (-)
• Length: 369 a.a.
• NCBI ID: WP_128872711.1
• Uniprot ID: A0A1V4LLX8
• Organism: Pseudomonas sp. MPC6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3879008..3890117

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ELQ88_RS19935 (ELQ88_19935)	-	3880041..3880814 (+)	774	WP_138967212.1	3-hydroxybutyrate dehydrogenase	-
ELQ88_RS19940 (ELQ88_19940)	-	3880939..3882894 (+)	1956	WP_138967214.1	acetoacetate--CoA ligase	-
ELQ88_RS19945 (ELQ88_19945)	pilU	3884008..3885117 (-)	1110	WP_128872711.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ELQ88_RS19950 (ELQ88_19950)	-	3885237..3887774 (+)	2538	WP_138967216.1	PAS domain-containing protein	-
ELQ88_RS19955 (ELQ88_19955)	-	3887867..3888142 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
ELQ88_RS19960 (ELQ88_19960)	-	3888272..3889204 (-)	933	WP_138967218.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40636.50 Da
• Isoelectric Point: 6.5710

>NTDB_id=333003 ELQ88_RS19945 WP_128872711.1 3884008..3885117(-) (pilU) [Pseudomonas sp. MPC6]
MEIAALLKVLASQDGSDLYLSTGAPPSARFDGVLKPLADQPFKPGEIAAIAASIMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSIVARNVKLDIPRFEDLKLPAVLLDAVMLKQGLMLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERRAQLLNDLGNNLKAFVSQRLVPSRDGHRRAAVEVMLGSPTIGDLIRRNEFSELKGIMEKSQEAGMQTFDGAL
YALVVEGAINEEEALKHADSVNNLRLRLKLHAEASPGTHAAPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=333003 ELQ88_RS19945 WP_128872711.1 3884008..3885117(-) (pilU) [Pseudomonas sp. MPC6]
ATGGAAATCGCTGCATTGTTGAAAGTCCTGGCCAGCCAGGACGGTTCCGATTTATACCTGTCCACGGGCGCGCCACCCAG
CGCGCGGTTCGATGGCGTGCTCAAACCCCTGGCCGATCAGCCGTTCAAACCCGGGGAAATCGCCGCTATTGCCGCGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGACCGGGAACTGGAGATGAACCTGGCAATCTCGTTGGCGGGTGTCGGGCGC
TTTCGGGTCAATATTTTCAAGCAACGCAACGATGTGTCGATCGTGGCGCGCAACGTCAAACTCGACATTCCGCGCTTCGA
AGACCTCAAGTTGCCCGCGGTGTTGCTCGATGCGGTCATGCTCAAGCAAGGCCTGATGCTCTTCGTCGGTGCCACCGGCT
CGGGCAAGTCAACCTCGCTGGCGGCACTGATCGACTACCGCAACCGCCACAGCAGCGGCCATATCATCACCATCGAAGAT
CCGGTCGAGTACATCCATCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTCGATACCCGCAGTTTTCATGC
CGCGCTGAAAAATACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGATACTGGCCATCTGGTGATCTCGACGCTGCATGCGCATAACGCCAATCAGGCACTGGACCGAATC
ATCAATTTTTTCCCCGAAGAACGCCGCGCGCAACTGCTCAATGACCTGGGGAATAACCTCAAGGCCTTCGTTTCCCAGCG
CTTGGTACCTTCGCGCGACGGTCACCGCCGGGCCGCGGTGGAGGTGATGCTGGGCTCGCCGACGATCGGCGATCTGATCC
GGCGCAACGAGTTTTCCGAGCTCAAGGGGATCATGGAAAAGTCGCAGGAGGCGGGGATGCAGACGTTCGACGGTGCGCTG
TATGCCTTGGTCGTCGAGGGGGCGATCAATGAGGAAGAGGCGCTCAAGCATGCGGATTCGGTGAACAACTTGCGGTTGCG
CTTGAAGTTGCATGCTGAAGCGTCACCGGGAACCCATGCCGCACCCGGTGAATGGGGACTCATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-244)

3D structure

• AlphaFold DB: A0A1V4LLX8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.707	99.729	0.556
pilU	Acinetobacter baylyi ADP1	51.821	96.748	0.501
pilU	Vibrio cholerae strain A1552	51.714	94.851	0.491
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	90.244	0.369
pilT	Vibrio cholerae strain A1552	40.841	90.244	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36