Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   EH197_RS09820 Genome accession   NZ_CP034169
Coordinates   1914591..1915028 (+) Length   145 a.a.
NCBI ID   WP_137439634.1    Uniprot ID   -
Organism   Enterococcus avium strain 352     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1905816..1950893 1914591..1915028 within 0


Gene organization within MGE regions


Location: 1905816..1950893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EH197_RS09750 (EH197_09750) - 1905816..1906952 (-) 1137 WP_137439613.1 site-specific integrase -
  EH197_RS09755 (EH197_09755) - 1907027..1907641 (-) 615 WP_137439615.1 DUF4352 domain-containing protein -
  EH197_RS09760 (EH197_09760) - 1907656..1908294 (-) 639 WP_137439617.1 XRE family transcriptional regulator -
  EH197_RS09765 (EH197_09765) - 1908450..1908680 (+) 231 WP_137439619.1 helix-turn-helix transcriptional regulator -
  EH197_RS09770 (EH197_09770) - 1908854..1909099 (+) 246 WP_137439620.1 hypothetical protein -
  EH197_RS09775 (EH197_09775) - 1909113..1909811 (+) 699 WP_137439622.1 Rha family transcriptional regulator -
  EH197_RS09780 (EH197_09780) - 1909822..1910013 (+) 192 WP_137439623.1 hypothetical protein -
  EH197_RS09785 (EH197_09785) - 1910046..1910516 (-) 471 WP_137440460.1 hypothetical protein -
  EH197_RS24460 - 1910606..1910752 (+) 147 WP_167494433.1 hypothetical protein -
  EH197_RS09790 (EH197_09790) - 1910788..1911015 (+) 228 WP_137439625.1 hypothetical protein -
  EH197_RS09795 (EH197_09795) - 1911028..1911564 (+) 537 WP_137439627.1 host-nuclease inhibitor Gam family protein -
  EH197_RS09800 (EH197_09800) - 1911566..1912564 (+) 999 WP_137439629.1 AAA family ATPase -
  EH197_RS09805 (EH197_09805) - 1912570..1913238 (+) 669 WP_137439631.1 putative HNHc nuclease -
  EH197_RS09810 (EH197_09810) - 1913249..1914181 (+) 933 WP_225844337.1 DnaD domain protein -
  EH197_RS09815 (EH197_09815) - 1914178..1914594 (+) 417 WP_137439633.1 RusA family crossover junction endodeoxyribonuclease -
  EH197_RS09820 (EH197_09820) ssb 1914591..1915028 (+) 438 WP_137439634.1 single-stranded DNA-binding protein Machinery gene
  EH197_RS09825 (EH197_09825) - 1915039..1915527 (+) 489 WP_137439636.1 hypothetical protein -
  EH197_RS09830 (EH197_09830) - 1915529..1915771 (+) 243 WP_048722687.1 hypothetical protein -
  EH197_RS09835 (EH197_09835) - 1915830..1916051 (+) 222 WP_137439637.1 hypothetical protein -
  EH197_RS24290 - 1916099..1916263 (+) 165 WP_158408725.1 hypothetical protein -
  EH197_RS09840 (EH197_09840) - 1916331..1916546 (+) 216 WP_158408726.1 DUF1642 domain-containing protein -
  EH197_RS24295 - 1916565..1916732 (+) 168 WP_158408727.1 hypothetical protein -
  EH197_RS09845 (EH197_09845) - 1916765..1917073 (+) 309 WP_137439641.1 hypothetical protein -
  EH197_RS09850 (EH197_09850) - 1917110..1917346 (+) 237 WP_070558084.1 hypothetical protein -
  EH197_RS24300 - 1917358..1917525 (+) 168 WP_158408728.1 hypothetical protein -
  EH197_RS09855 (EH197_09855) - 1917522..1917833 (+) 312 WP_102865907.1 replicase -
  EH197_RS24305 - 1917830..1917985 (+) 156 WP_158408729.1 hypothetical protein -
  EH197_RS09860 (EH197_09860) - 1918026..1918208 (+) 183 WP_070504224.1 hypothetical protein -
  EH197_RS09865 (EH197_09865) - 1918239..1919189 (+) 951 WP_070509400.1 site-specific integrase -
  EH197_RS09870 (EH197_09870) - 1919204..1919731 (+) 528 WP_028020911.1 hypothetical protein -
  EH197_RS09875 (EH197_09875) - 1919706..1920299 (+) 594 WP_028020912.1 N-6 DNA methylase -
  EH197_RS09880 (EH197_09880) - 1920332..1920919 (+) 588 WP_137439643.1 DUF1642 domain-containing protein -
  EH197_RS09885 (EH197_09885) - 1920928..1921317 (+) 390 WP_137439645.1 hypothetical protein -
  EH197_RS09890 (EH197_09890) - 1921314..1921577 (+) 264 WP_137439647.1 hypothetical protein -
  EH197_RS09895 (EH197_09895) - 1921574..1921753 (+) 180 WP_070621694.1 hypothetical protein -
  EH197_RS09900 (EH197_09900) - 1922011..1922400 (+) 390 WP_137439649.1 YopX family protein -
  EH197_RS09905 (EH197_09905) - 1922401..1922706 (+) 306 WP_048719862.1 hypothetical protein -
  EH197_RS09910 (EH197_09910) - 1922838..1923287 (+) 450 WP_158408730.1 hypothetical protein -
  EH197_RS24310 - 1923892..1924059 (+) 168 WP_158408731.1 hypothetical protein -
  EH197_RS09920 (EH197_09920) - 1924146..1924304 (+) 159 WP_010743339.1 hypothetical protein -
  EH197_RS24465 - 1924455..1924631 (+) 177 WP_167494434.1 hypothetical protein -
  EH197_RS09925 (EH197_09925) - 1924637..1924840 (+) 204 WP_137439655.1 hypothetical protein -
  EH197_RS09930 (EH197_09930) - 1924837..1925025 (+) 189 WP_137439656.1 hypothetical protein -
  EH197_RS09935 (EH197_09935) - 1925025..1925591 (+) 567 WP_244299452.1 hypothetical protein -
  EH197_RS09940 (EH197_09940) - 1925584..1925895 (+) 312 WP_137439658.1 HNH endonuclease -
  EH197_RS09945 (EH197_09945) - 1925889..1926191 (+) 303 WP_244299454.1 hypothetical protein -
  EH197_RS09950 (EH197_09950) - 1926315..1926674 (+) 360 WP_137439660.1 hypothetical protein -
  EH197_RS09955 (EH197_09955) - 1926671..1928314 (+) 1644 WP_137439661.1 terminase TerL endonuclease subunit -
  EH197_RS09960 (EH197_09960) - 1928340..1929494 (+) 1155 WP_137439663.1 phage portal protein -
  EH197_RS09965 (EH197_09965) - 1929475..1930182 (+) 708 WP_010722457.1 head maturation protease, ClpP-related -
  EH197_RS09970 (EH197_09970) - 1930209..1931345 (+) 1137 WP_137439665.1 phage major capsid protein -
  EH197_RS24470 - 1931353..1931496 (+) 144 WP_002342053.1 hypothetical protein -
  EH197_RS09975 (EH197_09975) - 1931509..1931796 (+) 288 WP_137439666.1 head-tail connector protein -
  EH197_RS09980 (EH197_09980) - 1931777..1932157 (+) 381 WP_104771611.1 phage head-tail adapter protein -
  EH197_RS09985 (EH197_09985) - 1932150..1932554 (+) 405 WP_137439667.1 hypothetical protein -
  EH197_RS09990 (EH197_09990) - 1932551..1932961 (+) 411 WP_137439668.1 hypothetical protein -
  EH197_RS09995 (EH197_09995) - 1933026..1933622 (+) 597 WP_137439669.1 major tail protein -
  EH197_RS10000 (EH197_10000) - 1933683..1933994 (+) 312 WP_137439670.1 hypothetical protein -
  EH197_RS10005 (EH197_10005) - 1934189..1940401 (+) 6213 WP_137439671.1 phage tail tape measure protein -
  EH197_RS10010 (EH197_10010) - 1940402..1941223 (+) 822 WP_137439672.1 phage tail domain-containing protein -
  EH197_RS10015 (EH197_10015) - 1941232..1942257 (+) 1026 WP_137439673.1 phage tail protein -
  EH197_RS10020 (EH197_10020) - 1942257..1943306 (+) 1050 WP_137439674.1 hypothetical protein -
  EH197_RS24795 - 1943307..1945976 (+) 2670 WP_244299456.1 BppU family phage baseplate upper protein -
  EH197_RS10035 (EH197_10035) - 1945987..1946337 (+) 351 WP_137439675.1 hypothetical protein -
  EH197_RS25040 - 1946338..1946451 (+) 114 WP_010746267.1 CD1375 family protein -
  EH197_RS25045 - 1946489..1946614 (+) 126 WP_010746268.1 CD1375 family protein -
  EH197_RS10040 (EH197_10040) - 1946670..1946912 (+) 243 WP_016180074.1 hemolysin XhlA family protein -
  EH197_RS10045 (EH197_10045) - 1946924..1947121 (+) 198 WP_010746270.1 phage holin -
  EH197_RS10050 (EH197_10050) - 1947132..1948241 (+) 1110 WP_137439676.1 GH25 family lysozyme -
  EH197_RS10055 (EH197_10055) - 1948288..1948542 (-) 255 WP_070621288.1 hypothetical protein -
  EH197_RS10065 (EH197_10065) - 1948723..1949121 (-) 399 WP_137439677.1 hypothetical protein -
  EH197_RS10070 (EH197_10070) - 1949518..1950387 (+) 870 WP_048720345.1 SPFH domain-containing protein -
  EH197_RS10075 (EH197_10075) - 1950399..1950893 (+) 495 WP_048720343.1 LURP-one-related family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16111.78 Da        Isoelectric Point: 5.2262

>NTDB_id=329520 EH197_RS09820 WP_137439634.1 1914591..1915028(+) (ssb) [Enterococcus avium strain 352]
MINNVVLVGRLTKDPDLRYTASGTGVATFTLAVNRNFTNQDGNRDADFINCVIWRKSAETLANYAKKGTLLGVIGRIQTR
SYENQQGQRVYVTEVVAENFQLLESRSTNENRQENKRPKSTDTTNASSDPFSGSSIDIGDDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=329520 EH197_RS09820 WP_137439634.1 1914591..1915028(+) (ssb) [Enterococcus avium strain 352]
ATGATCAACAATGTGGTTTTAGTCGGGAGATTAACAAAAGATCCTGACTTACGATACACAGCGAGTGGCACGGGAGTAGC
AACATTCACTCTTGCAGTGAACAGAAACTTTACCAATCAAGATGGTAACAGAGATGCAGACTTTATTAACTGCGTAATTT
GGCGGAAGTCAGCAGAGACGTTAGCCAATTATGCTAAAAAAGGAACGCTGTTAGGTGTGATTGGCCGCATTCAAACAAGA
AGCTATGAAAATCAGCAAGGACAACGTGTCTATGTAACCGAAGTAGTTGCTGAGAACTTCCAATTGCTTGAATCACGTAG
TACTAATGAGAATCGGCAAGAGAATAAGAGGCCAAAGAGTACCGATACAACAAATGCAAGTTCTGATCCTTTTAGTGGTT
CATCGATTGACATCGGCGACGATGACTTGCCGTTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

58.824

100

0.69

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.598

100

0.655

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.828

100

0.448

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

73.103

0.434

  ssbB/cilA Streptococcus pneumoniae Rx1

42.759

100

0.428

  ssbB/cilA Streptococcus pneumoniae D39

42.759

100

0.428

  ssbB/cilA Streptococcus pneumoniae R6

42.759

100

0.428

  ssbB/cilA Streptococcus mitis SK321

42.759

100

0.428

  ssbB/cilA Streptococcus pneumoniae TIGR4

50.435

79.31

0.4

  ssbB/cilA Streptococcus mitis NCTC 12261

49.565

79.31

0.393

  ssbA Streptococcus mutans UA159

46.087

79.31

0.366


Multiple sequence alignment