Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EFI48_RS02165 Genome accession   NZ_CP033604
Coordinates   451910..453151 (-) Length   413 a.a.
NCBI ID   WP_005355091.1    Uniprot ID   A0A2T4MY12
Organism   Aeromonas veronii strain MS-18-37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446910..458151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFI48_RS02140 (EFI48_02140) mutT 448902..449306 (+) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -
  EFI48_RS02145 (EFI48_02145) yacG 449384..449578 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  EFI48_RS02150 (EFI48_02150) zapD 449588..450310 (-) 723 WP_164461667.1 cell division protein ZapD -
  EFI48_RS02155 (EFI48_02155) coaE 450348..450962 (-) 615 WP_123172236.1 dephospho-CoA kinase -
  EFI48_RS02160 (EFI48_02160) pilD 450976..451848 (-) 873 WP_123172237.1 prepilin peptidase Machinery gene
  EFI48_RS02165 (EFI48_02165) pilC 451910..453151 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  EFI48_RS02170 (EFI48_02170) pilB 453391..455097 (-) 1707 WP_123172238.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EFI48_RS02175 (EFI48_02175) - 455097..455564 (-) 468 WP_123172239.1 pilin -
  EFI48_RS02185 (EFI48_02185) nadC 455880..456755 (-) 876 WP_123172240.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EFI48_RS02190 (EFI48_02190) - 456759..457229 (-) 471 WP_005336129.1 retropepsin-like aspartic protease family protein -
  EFI48_RS02195 (EFI48_02195) ampD 457360..457935 (+) 576 WP_123172241.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45441.57 Da        Isoelectric Point: 9.7221

>NTDB_id=324258 EFI48_RS02165 WP_005355091.1 451910..453151(-) (pilC) [Aeromonas veronii strain MS-18-37]
MATLTKKNNAPKKVFAFRWHGVNRKGQKVSGELQADSINTVKTELRKQGVNVTKVAKKSQGLFSKGGAKIKPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKASMRELMGQIAADVETGTPMSEALRRHPLYFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPVFTQFVIGISRFMQNWWYVFFGGTA
LGIFLYVRAWRASQKVKDNTDKFVLTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVIA
MYLPIFKLGEVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=324258 EFI48_RS02165 WP_005355091.1 451910..453151(-) (pilC) [Aeromonas veronii strain MS-18-37]
ATGGCAACCCTAACGAAAAAGAACAATGCCCCCAAGAAAGTCTTCGCCTTCCGCTGGCACGGGGTAAACCGCAAGGGGCA
GAAGGTCTCCGGCGAGCTACAGGCCGACAGCATCAACACCGTCAAGACCGAACTGCGCAAGCAGGGGGTCAACGTCACCA
AGGTGGCCAAAAAATCCCAAGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAACCGATGGATATCGCCATCGTCTCACGC
CAAATAACTACCATGCTCTCCGCCGGTGTACCGCTGGTGCAAAGCCTGCAGATTATCGCCCGCAGTCACGAGAAAGCCTC
GATGCGCGAGCTGATGGGGCAGATTGCCGCCGATGTGGAGACCGGTACCCCCATGTCGGAGGCGCTGCGCCGCCATCCCC
TCTACTTTGATGATCTCTATTGCGATCTTGTGGAAGCCGGTGAGCAATCCGGTGCGCTGGAGACCATCTACGACCGTATC
GCCACCTATCGGGAAAAGTCGGAAGCGCTCAAATCGAAGATCAAGAAGGCAATGTTCTACCCCACCATGGTCATTCTGGT
CGCCATTGTGGTCACCTCCATCCTGCTGCTGTTCGTCATTCCGCAGTTCGAGGATATCTTCAAGAGCTTCGGTGCCGAGC
TGCCGGTGTTCACCCAGTTTGTTATCGGCATCTCCCGCTTTATGCAGAACTGGTGGTATGTCTTCTTTGGTGGCACCGCC
CTTGGCATCTTCCTCTATGTGCGGGCCTGGCGGGCCTCCCAGAAGGTGAAAGACAATACCGACAAGTTCGTCCTCACCAT
TCCGGTGGTCGGGATGATCCTGCACAAGGCGGCGATGGCTCGCTTTGCCCGTACTCTTTCAACCACTTTCTCTGCCGGTA
TTCCGCTGGTAGATGCGCTGATTTCAGCAGCAGGGGCCTCCGGCAACTATGTCTATCGCACCGCCGTCATGGCGATCCGC
AACGAGGTGGTGGCAGGCATGCAGATCAACGTGGCAATGCGCACCGTCGATCTCTTTCCCGACATGGTGATCCAAATGGT
GATGATCGGTGAGGAGTCCGGAGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAACAGGAGGTGGATGATC
TGGTCGATGGCCTCACCAGCCTGCTGGAACCCCTCATCATGGTGGTGCTCGGGGTGCTGGTCGGCGGCATGGTTATTGCC
ATGTACCTGCCCATCTTCAAGCTTGGCGAAGTGGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4MY12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

53.75

96.852

0.521
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Legionella pneumophila strain ERS1305867

50.985

98.305

0.501
  pilC Vibrio cholerae strain A1552

46.883

97.094

0.455
  pilC Vibrio campbellii strain DS40M4

45.792

97.821

0.448
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment