Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EFI48_RS02170 Genome accession   NZ_CP033604
Coordinates   453391..455097 (-) Length   568 a.a.
NCBI ID   WP_123172238.1    Uniprot ID   A0AAN1QCI3
Organism   Aeromonas veronii strain MS-18-37     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448391..460097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFI48_RS02140 (EFI48_02140) mutT 448902..449306 (+) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -
  EFI48_RS02145 (EFI48_02145) yacG 449384..449578 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  EFI48_RS02150 (EFI48_02150) zapD 449588..450310 (-) 723 WP_164461667.1 cell division protein ZapD -
  EFI48_RS02155 (EFI48_02155) coaE 450348..450962 (-) 615 WP_123172236.1 dephospho-CoA kinase -
  EFI48_RS02160 (EFI48_02160) pilD 450976..451848 (-) 873 WP_123172237.1 prepilin peptidase Machinery gene
  EFI48_RS02165 (EFI48_02165) pilC 451910..453151 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  EFI48_RS02170 (EFI48_02170) pilB 453391..455097 (-) 1707 WP_123172238.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EFI48_RS02175 (EFI48_02175) - 455097..455564 (-) 468 WP_123172239.1 pilin -
  EFI48_RS02185 (EFI48_02185) nadC 455880..456755 (-) 876 WP_123172240.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EFI48_RS02190 (EFI48_02190) - 456759..457229 (-) 471 WP_005336129.1 retropepsin-like aspartic protease family protein -
  EFI48_RS02195 (EFI48_02195) ampD 457360..457935 (+) 576 WP_123172241.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EFI48_RS02200 (EFI48_02200) pdhR 458214..458978 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62576.90 Da        Isoelectric Point: 5.3170

>NTDB_id=324259 EFI48_RS02170 WP_123172238.1 453391..455097(-) (pilB) [Aeromonas veronii strain MS-18-37]
MTSNPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLTEIPQKY
LNQKLIDKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=324259 EFI48_RS02170 WP_123172238.1 453391..455097(-) (pilB) [Aeromonas veronii strain MS-18-37]
ATGACATCTAATCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATTAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGACCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATCGACAAGCACCATGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTAGAGGAAAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGTGCCTCCGACCTGCACTTTGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTTTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCTTGCCCACCATGTGGGGGGAGAAGATCGTGATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGATCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAA
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTGAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCTTGCGGCAAGACCCGGATGTAGTGATGGTGGGGGAAATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCAGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTATGCGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCAGCTGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGTACCTTACGTACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTAGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.578

98.063

0.535
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.418

99.296

0.511
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment