Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PLES_RS27935 Genome accession   NC_011770
Coordinates   6010391..6012535 (-) Length   714 a.a.
NCBI ID   WP_012614629.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa LESB58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6005391..6017535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PLES_RS27920 (PLES_54271) - 6007049..6008698 (-) 1650 WP_003116139.1 AAA family ATPase -
  PLES_RS27925 (PLES_54281) aroB 6008705..6009811 (-) 1107 WP_012614628.1 3-dehydroquinate synthase -
  PLES_RS27930 (PLES_54291) aroK 6009861..6010379 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PLES_RS27935 (PLES_54301) pilQ 6010391..6012535 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  PLES_RS27940 (PLES_54311) pilP 6012589..6013113 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  PLES_RS27945 (PLES_54321) pilO 6013110..6013733 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  PLES_RS27950 (PLES_54331) pilN 6013730..6014326 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  PLES_RS27955 (PLES_54341) pilM 6014326..6015390 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77401.96 Da        Isoelectric Point: 5.3214

>NTDB_id=32374 PLES_RS27935 WP_012614629.1 6010391..6012535(-) (pilQ) [Pseudomonas aeruginosa LESB58]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
RGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=32374 PLES_RS27935 WP_012614629.1 6010391..6012535(-) (pilQ) [Pseudomonas aeruginosa LESB58]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAACCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGATGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAACTGATCGCCGACTTCACCGACCTGAATCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTACTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTACTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGCCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGAGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAACCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGAATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGTGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAC
GAGGTGAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

98.179

100

0.982
  pilQ Acinetobacter baumannii D1279779

41.268

99.44

0.41
  pilQ Acinetobacter baumannii strain A118

41.268

99.44

0.41
  comQ Acinetobacter baylyi ADP1

41.738

98.319

0.41

Multiple sequence alignment