Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EAW52_RS17370 Genome accession   NZ_CP033105
Coordinates   3717144..3718361 (+) Length   405 a.a.
NCBI ID   WP_122043367.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3712144..3723361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS17355 (EAW52_17355) - 3713193..3714527 (-) 1335 WP_162997654.1 O-antigen ligase -
  EAW52_RS17360 (EAW52_17360) - 3714655..3715107 (-) 453 WP_122044842.1 pilin -
  EAW52_RS17365 (EAW52_17365) pilB 3715327..3717012 (+) 1686 WP_122043366.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAW52_RS17370 (EAW52_17370) pilC 3717144..3718361 (+) 1218 WP_122043367.1 type II secretion system F family protein Machinery gene
  EAW52_RS17375 (EAW52_17375) pilD 3718361..3719230 (+) 870 WP_122043368.1 A24 family peptidase Machinery gene
  EAW52_RS17380 (EAW52_17380) coaE 3719230..3719844 (+) 615 WP_122043369.1 dephospho-CoA kinase -
  EAW52_RS17385 (EAW52_17385) yacG 3719837..3720040 (+) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  EAW52_RS17390 (EAW52_17390) - 3720131..3720604 (-) 474 WP_010797203.1 hypothetical protein -
  EAW52_RS17395 (EAW52_17395) - 3720808..3721290 (+) 483 WP_122043370.1 MOSC domain-containing protein -
  EAW52_RS17400 (EAW52_17400) - 3721396..3722694 (+) 1299 WP_122043371.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44297.06 Da        Isoelectric Point: 9.6385

>NTDB_id=321415 EAW52_RS17370 WP_122043367.1 3717144..3718361(+) (pilC) [Pseudomonas sp. LTJR-52]
MADKALKMSAFTWEGTDRKGVKIKGEMTGLNPALVKAQLRKQGINPTRVRKKSVSIFSRGKKIKPMDIALFTRQLATMLG
AGVPLLQSFDIISEGFDNPNMRKLVDDVKQDVAAGNSLSSSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
EQLKKKIKKAMTYPAAVVVVAIVVSAILLIKVVPQFQSVFEGFGAELPAFTLMVIGLSETLQEWWFIVLLALFALGFGFQ
MAHKRSEKFRDAVDRFVLKIPVVGAILYKSAIARYARTLSTTFAAGVPLVDALDSVSGATGNVVFRNAVDKIKQDVSTGM
QLNFSMRTTAVFTSMAVQMTAIGEESGALDEMLDKVATFYEEEVDNMVDSLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=321415 EAW52_RS17370 WP_122043367.1 3717144..3718361(+) (pilC) [Pseudomonas sp. LTJR-52]
ATGGCCGACAAAGCATTAAAAATGAGCGCTTTTACCTGGGAGGGAACGGACCGTAAAGGCGTCAAGATCAAGGGCGAAAT
GACCGGGCTGAACCCTGCCCTGGTCAAGGCCCAACTGCGCAAGCAAGGGATCAACCCCACGCGCGTACGCAAAAAATCAG
TCTCGATCTTCAGCCGCGGCAAAAAGATCAAGCCGATGGACATCGCCCTGTTCACTCGTCAGCTAGCCACTATGCTGGGG
GCTGGCGTTCCTTTGCTGCAGTCGTTCGACATTATCAGCGAAGGCTTTGATAACCCGAACATGCGCAAGCTGGTCGATGA
CGTCAAGCAGGACGTGGCAGCGGGTAACAGCCTGTCTAGCTCGCTGCGTAAAAAGCCTCAATACTTCGATGATCTCTACT
GCAACCTAGTCGATGCGGGTGAGCAGTCTGGTGCGCTGGAAACGCTGCTGGATCGCGTAGCGACCTACAAAGAAAAAACC
GAGCAGCTCAAGAAAAAAATCAAAAAGGCTATGACCTACCCTGCCGCGGTTGTAGTGGTAGCGATTGTGGTTTCAGCCAT
TCTATTGATCAAGGTTGTACCTCAGTTCCAATCCGTATTCGAGGGCTTTGGTGCAGAGCTACCTGCGTTTACATTAATGG
TGATTGGCCTTTCAGAAACCCTGCAAGAATGGTGGTTTATCGTTCTGCTGGCTCTTTTCGCTTTAGGTTTCGGCTTTCAG
ATGGCACACAAACGGTCTGAAAAATTCCGCGATGCCGTTGACCGTTTTGTACTCAAAATACCAGTCGTGGGCGCCATTCT
TTATAAGTCTGCGATTGCACGCTATGCGCGCACCTTATCCACCACCTTTGCTGCAGGCGTACCGCTGGTGGATGCATTGG
ATTCGGTTTCAGGCGCTACGGGTAACGTTGTCTTCCGCAACGCGGTCGACAAGATCAAGCAAGATGTCTCGACCGGCATG
CAGCTGAACTTCTCCATGCGAACCACTGCCGTATTTACCTCTATGGCAGTGCAAATGACGGCCATTGGTGAAGAGTCGGG
TGCACTGGACGAAATGCTGGATAAAGTGGCCACGTTTTATGAAGAAGAAGTGGACAACATGGTCGATAGCCTTACCGCTC
TGATGGAGCCCATGATCATGGCGGTTCTGGGCGTACTGGTGGGCGGTTTGATCATCGCCATGTACCTGCCTATCTTCCAG
TTGGGCTCGGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

81.235

100

0.812
  pilC Acinetobacter baumannii D1279779

61.386

99.753

0.612
  pilC Acinetobacter baylyi ADP1

60.837

100

0.61
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

44.828

100

0.449
  pilG Neisseria gonorrhoeae MS11

44.828

100

0.449
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

38.958

99.506

0.388

Multiple sequence alignment