Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EAW52_RS17375 Genome accession   NZ_CP033105
Coordinates   3718361..3719230 (+) Length   289 a.a.
NCBI ID   WP_122043368.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3713361..3724230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS17360 (EAW52_17360) - 3714655..3715107 (-) 453 WP_122044842.1 pilin -
  EAW52_RS17365 (EAW52_17365) pilB 3715327..3717012 (+) 1686 WP_122043366.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAW52_RS17370 (EAW52_17370) pilC 3717144..3718361 (+) 1218 WP_122043367.1 type II secretion system F family protein Machinery gene
  EAW52_RS17375 (EAW52_17375) pilD 3718361..3719230 (+) 870 WP_122043368.1 A24 family peptidase Machinery gene
  EAW52_RS17380 (EAW52_17380) coaE 3719230..3719844 (+) 615 WP_122043369.1 dephospho-CoA kinase -
  EAW52_RS17385 (EAW52_17385) yacG 3719837..3720040 (+) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  EAW52_RS17390 (EAW52_17390) - 3720131..3720604 (-) 474 WP_010797203.1 hypothetical protein -
  EAW52_RS17395 (EAW52_17395) - 3720808..3721290 (+) 483 WP_122043370.1 MOSC domain-containing protein -
  EAW52_RS17400 (EAW52_17400) - 3721396..3722694 (+) 1299 WP_122043371.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31927.84 Da        Isoelectric Point: 6.6804

>NTDB_id=321416 EAW52_RS17375 WP_122043368.1 3718361..3719230(+) (pilD) [Pseudomonas sp. LTJR-52]
MEAIELLATYPAAYLSCALVLGLLVGSFLNVVVHRLPKMLERDWIAQSRQMLELPSEGEAEPYNLAKPHSHCPHCQHRIR
PWENIPVISYIMLRGKCSSCKTPISARYPLVELACGLLSLFVVWHFGFTWQAGSALLLTWGLLAASLIDADHQILPDVIV
LPLLWVGLLVNQFELFSSLSDAVWGAAAGYMSLWVVFQLFRLITGKEGMGFGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGIILLRLKNAENSTPIPFGPYLAIAGWIALLWGDQITGMYLHFAGF

Nucleotide


Download         Length: 870 bp        

>NTDB_id=321416 EAW52_RS17375 WP_122043368.1 3718361..3719230(+) (pilD) [Pseudomonas sp. LTJR-52]
ATGGAGGCTATCGAGCTTTTGGCAACCTACCCCGCCGCGTACTTGAGCTGTGCGTTGGTACTGGGCTTATTGGTTGGCAG
CTTTCTCAATGTGGTCGTACACCGGCTCCCCAAGATGCTTGAGCGGGACTGGATAGCCCAGTCCCGCCAAATGCTGGAAC
TACCCAGCGAAGGCGAAGCGGAACCCTATAACCTTGCCAAGCCTCACTCCCATTGCCCTCACTGCCAACATCGCATTCGG
CCGTGGGAAAACATTCCTGTTATCAGCTATATCATGCTGCGCGGCAAGTGCTCCAGCTGCAAGACCCCTATCAGCGCTCG
CTATCCGCTGGTTGAATTGGCGTGCGGCCTGCTTTCCCTGTTTGTTGTCTGGCATTTTGGCTTTACCTGGCAGGCTGGCA
GCGCCTTGTTACTGACCTGGGGGCTGCTCGCCGCCAGCCTGATCGATGCTGATCACCAAATTCTTCCAGACGTCATTGTT
CTTCCGCTGCTATGGGTTGGCTTGCTGGTTAACCAGTTTGAGTTGTTCTCTAGTCTCTCCGATGCAGTCTGGGGCGCAGC
AGCGGGTTATATGAGCTTGTGGGTGGTTTTCCAGCTTTTCAGGCTGATCACAGGCAAGGAAGGCATGGGCTTTGGTGACT
TCAAGCTGCTGGCCATGCTGGGCGCCTGGGGCGGCTGGCAAATCCTGCCGCTTACGATATTGCTGTCCTCTTTAGTGGGT
GCGATTCTCGGCATCATTCTGCTTCGCCTCAAGAATGCCGAAAACAGCACCCCGATACCGTTTGGGCCTTATCTAGCCAT
TGCGGGGTGGATTGCTTTGCTTTGGGGTGATCAAATAACAGGCATGTACCTTCACTTTGCCGGTTTTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.273

95.156

0.526
  pilD Vibrio cholerae strain A1552

52.797

98.962

0.522
  pilD Acinetobacter nosocomialis M2

54.182

95.156

0.516
  pilD Vibrio campbellii strain DS40M4

49.117

97.924

0.481
  pilD Neisseria gonorrhoeae MS11

48.669

91.003

0.443

Multiple sequence alignment