Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EAW52_RS17365 Genome accession   NZ_CP033105
Coordinates   3715327..3717012 (+) Length   561 a.a.
NCBI ID   WP_122043366.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3710327..3722012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS17345 (EAW52_17345) - 3712049..3712999 (+) 951 WP_122043364.1 glycosyltransferase family 2 protein -
  EAW52_RS17355 (EAW52_17355) - 3713193..3714527 (-) 1335 WP_162997654.1 O-antigen ligase -
  EAW52_RS17360 (EAW52_17360) - 3714655..3715107 (-) 453 WP_122044842.1 pilin -
  EAW52_RS17365 (EAW52_17365) pilB 3715327..3717012 (+) 1686 WP_122043366.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAW52_RS17370 (EAW52_17370) pilC 3717144..3718361 (+) 1218 WP_122043367.1 type II secretion system F family protein Machinery gene
  EAW52_RS17375 (EAW52_17375) pilD 3718361..3719230 (+) 870 WP_122043368.1 A24 family peptidase Machinery gene
  EAW52_RS17380 (EAW52_17380) coaE 3719230..3719844 (+) 615 WP_122043369.1 dephospho-CoA kinase -
  EAW52_RS17385 (EAW52_17385) yacG 3719837..3720040 (+) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  EAW52_RS17390 (EAW52_17390) - 3720131..3720604 (-) 474 WP_010797203.1 hypothetical protein -
  EAW52_RS17395 (EAW52_17395) - 3720808..3721290 (+) 483 WP_122043370.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62166.36 Da        Isoelectric Point: 5.5935

>NTDB_id=321414 EAW52_RS17365 WP_122043366.1 3715327..3717012(+) (pilB) [Pseudomonas sp. LTJR-52]
MNDLAPLSGLARQLVLAELLDEKTAQSAVAQSKISKVPLVTHLVQNRLVKSRAIAELSAEQFGLAFLDLSAVDKESLPKE
VVSEKLMQQHHFLPLAKRGNKLFIGISDPTNQQAINDIQFSTGLTAEPILVEDDKLHNAIDQYLDNGTGGLDLGDIDLDI
EINTEEEKKSEVDADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKMYRVRFRTDGVMHEMARPPIQLAPRISARLKVMAS
MDISERRKPQDGRVKLKVSKNKSIDFRVNTLPTLWGEKIVMRILDPSTAQMGIDALGYEERQKELYMEALSQPQGMILVT
GPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGIDFAQALRAFLRQDPDVIMVGEIRDLETAEI
AIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPKCKKPHDVPRETLLSEGFPESQIGK
FTLYSPIGCEDCNNGYRGRVGIYEVVKITSAMQNLIMEEGNSIEIARKAREEGFNDLRTSGLLKAMQGVTSLEEVNRVTK
D

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=321414 EAW52_RS17365 WP_122043366.1 3715327..3717012(+) (pilB) [Pseudomonas sp. LTJR-52]
ATGAATGATTTAGCGCCTCTCTCTGGTCTTGCCCGTCAGCTTGTTTTAGCCGAGCTACTCGATGAAAAAACGGCTCAAAG
CGCAGTTGCGCAGAGCAAGATCAGTAAAGTCCCACTGGTGACACACCTAGTTCAAAATCGCCTCGTTAAAAGCCGTGCGA
TTGCGGAGCTGTCTGCAGAACAATTCGGCTTGGCGTTTTTAGACCTCAGCGCAGTAGATAAGGAGTCCTTGCCTAAAGAG
GTCGTGAGCGAGAAGTTGATGCAACAGCATCACTTTCTGCCGCTTGCCAAACGGGGTAACAAGCTTTTTATCGGCATATC
CGACCCAACCAACCAGCAGGCCATTAATGACATTCAGTTCAGTACCGGCCTTACGGCTGAGCCTATTCTGGTTGAGGATG
ACAAGCTTCATAACGCCATTGATCAGTACCTGGATAACGGCACCGGTGGGCTGGATCTTGGTGATATTGATCTTGATATC
GAGATCAATACAGAAGAAGAAAAAAAGTCTGAAGTAGATGCGGACGATGCCCCGGTTGTACGCTTCGTCAACAAGATGCT
GCTCGACGCTATCAAGCGCGGCTCGTCTGACCTGCACTTTGAGCCTTACGAGAAAATGTATCGAGTGCGTTTTCGTACCG
ATGGCGTCATGCATGAAATGGCACGCCCGCCTATTCAGCTGGCCCCACGCATTTCGGCCCGTCTGAAGGTAATGGCGAGT
ATGGATATTTCCGAGCGCCGCAAGCCGCAGGATGGTCGCGTGAAGCTCAAGGTTTCCAAAAACAAGTCCATCGATTTTCG
TGTCAACACGCTTCCGACCCTCTGGGGCGAGAAGATTGTGATGCGAATTCTCGATCCCTCCACTGCGCAGATGGGTATTG
ATGCGTTGGGCTATGAAGAGCGCCAGAAAGAGCTTTATATGGAGGCGCTTAGCCAACCACAGGGCATGATTCTGGTAACC
GGCCCCACTGGCTCTGGCAAAACCGTATCGCTGTACACAGGCTTGAACATCCTTAATACAGTCGACATCAATATCTCGAC
GGCAGAAGATCCGGTAGAGATCAACCTGGAGGGTATTAACCAGGTTAACGTTAACCCGCGTCAAGGAATAGACTTCGCCC
AAGCGCTTAGAGCGTTTCTCCGTCAAGATCCTGACGTGATCATGGTGGGCGAGATCCGCGATCTGGAAACAGCAGAGATT
GCCATAAAGGCTGCACAAACGGGTCACATGGTTATGTCGACGCTGCATACCAACAGCGCGGCAGAAACACTGACGCGCCT
GCGGAACATGGGCGTACCTGCTTTTAACATCGCCACATCGGTCAACCTGATCATTGCTCAACGATTGGCACGCAAGCTGT
GCCCTAAATGTAAAAAGCCACATGATGTACCAAGAGAAACCTTGTTGAGTGAAGGTTTCCCCGAAAGCCAGATCGGCAAG
TTTACGCTGTACTCACCTATTGGTTGCGAGGATTGCAATAACGGCTACCGTGGCCGTGTGGGTATTTATGAAGTCGTGAA
GATCACATCTGCCATGCAGAACCTGATCATGGAAGAAGGCAACTCGATCGAGATTGCCCGCAAGGCGCGTGAAGAGGGCT
TTAATGACCTACGGACATCTGGCCTGCTCAAGGCCATGCAAGGCGTAACCAGCCTGGAAGAAGTAAACCGCGTCACCAAG
GACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.289

100

0.583
  pilB Acinetobacter baumannii D1279779

58.007

100

0.581
  pilB Legionella pneumophila strain ERS1305867

53.263

100

0.538
  pilB Vibrio cholerae strain A1552

49.911

100

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

49.64

99.109

0.492
  pilF Neisseria gonorrhoeae MS11

49.013

99.287

0.487
  pilB Vibrio campbellii strain DS40M4

48.49

100

0.487
  pilB/pilB1 Synechocystis sp. PCC 6803

40.039

92.157

0.369
  pilF Thermus thermophilus HB27

36.964

99.822

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

95.9

0.365

Multiple sequence alignment