Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EAG14_RS17270 Genome accession   NZ_CP033069
Coordinates   3885931..3887148 (+) Length   405 a.a.
NCBI ID   WP_121729618.1    Uniprot ID   -
Organism   Acidovorax sp. 1608163     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3880931..3892148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAG14_RS17250 (EAG14_17250) rpmA 3882178..3882435 (-) 258 WP_026437555.1 50S ribosomal protein L27 -
  EAG14_RS17255 (EAG14_17255) rplU 3882451..3882762 (-) 312 WP_010462012.1 50S ribosomal protein L21 -
  EAG14_RS17260 (EAG14_17260) - 3883005..3883901 (+) 897 WP_240456818.1 polyprenyl synthetase family protein -
  EAG14_RS17265 (EAG14_17265) pilB 3884174..3885907 (+) 1734 WP_121729617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAG14_RS17270 (EAG14_17270) pilC 3885931..3887148 (+) 1218 WP_121729618.1 type II secretion system F family protein Machinery gene
  EAG14_RS17275 (EAG14_17275) pilD 3887148..3888020 (+) 873 WP_121729619.1 A24 family peptidase Machinery gene
  EAG14_RS17280 (EAG14_17280) coaE 3888023..3888637 (+) 615 WP_121729620.1 dephospho-CoA kinase -
  EAG14_RS17285 (EAG14_17285) zapD 3888727..3889482 (+) 756 WP_121729621.1 cell division protein ZapD -
  EAG14_RS17290 (EAG14_17290) - 3889515..3889748 (+) 234 WP_121456223.1 DNA gyrase inhibitor YacG -
  EAG14_RS17295 (EAG14_17295) - 3889816..3890223 (-) 408 WP_121729622.1 NUDIX domain-containing protein -
  EAG14_RS17300 (EAG14_17300) - 3890304..3891170 (-) 867 WP_121729623.1 ATP-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44550.47 Da        Isoelectric Point: 9.5480

>NTDB_id=321150 EAG14_RS17270 WP_121729618.1 3885931..3887148(+) (pilC) [Acidovorax sp. 1608163]
MATAASRDIKEFVFEWEGKDRAGKVVRGEIRAVGENQVQATLRRQGVFPTKIKKRRTRSGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNASVTKLLNDIRTDVETGTSLNAAFRKHPMYFDSLYCNLVEAGEAAGILEALLDRLATYMEKT
EAIKSKIKSALMYPISVVIVAFVVVTVIMIFVIPAFKEVFSSFGADLPAPTLLVMGISEVFVKWWWLIFSIIGGGFYFFM
QAWKRSEKMQKFMDRFLLKMPIFGALIDKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNSVYSTATEKIQQEVSTGT
SLTVAMDNAKVFPSMVIQMCAIGEESGSIDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=321150 EAG14_RS17270 WP_121729618.1 3885931..3887148(+) (pilC) [Acidovorax sp. 1608163]
ATGGCAACCGCAGCATCAAGGGACATCAAAGAGTTTGTCTTTGAATGGGAAGGCAAGGACCGCGCAGGCAAGGTGGTGCG
CGGGGAGATTCGGGCTGTCGGCGAAAACCAGGTTCAGGCCACGCTGCGCCGACAAGGCGTTTTTCCGACCAAAATCAAGA
AGAGGCGTACCCGTTCCGGCAAGAAGATCAAGCCCAAAGACATTGCACTCTTCACCCGGCAATTGGCCACCATGATGAAG
GCCGGTGTTCCACTACTGCAGTCTTTTGACATTGTCGGGCGTGGCAACACCAACGCCAGCGTGACCAAACTGTTGAATGA
CATTCGTACCGATGTTGAGACCGGCACCTCATTGAACGCCGCGTTTCGCAAGCACCCCATGTACTTCGACAGCCTGTATT
GCAACTTGGTGGAAGCCGGGGAAGCGGCAGGTATTCTTGAAGCGCTGCTGGACCGACTTGCGACTTACATGGAAAAGACA
GAGGCGATCAAATCCAAGATCAAATCAGCCTTGATGTACCCGATCTCGGTGGTCATTGTTGCCTTTGTGGTGGTGACTGT
GATCATGATTTTCGTGATCCCAGCATTCAAGGAGGTCTTCTCGTCTTTTGGAGCAGATCTGCCAGCTCCCACGCTGCTTG
TGATGGGCATCAGCGAGGTATTTGTTAAATGGTGGTGGCTGATCTTCTCCATCATTGGTGGCGGGTTCTATTTTTTCATG
CAAGCCTGGAAGCGCAGTGAAAAAATGCAGAAATTCATGGACCGCTTTCTGCTCAAGATGCCCATTTTTGGCGCCTTGAT
TGATAAGTCTTGCGTAGCCCGCTGGACGCGAACCTTGGCCACCATGTTTGCAGCAGGTGTTCCACTGGTGGAAGCGCTTG
ACTCCGTCGGTGGCGCCTCGGGGAACTCGGTGTACTCCACGGCGACCGAGAAAATCCAGCAAGAAGTCTCCACCGGCACT
AGCCTTACCGTCGCAATGGACAACGCCAAGGTATTTCCCTCCATGGTGATCCAGATGTGCGCCATCGGTGAGGAATCCGG
CTCCATTGATCACATGCTCGGCAAAGCCGCAGACTTTTACGAGGCCGAAGTGGACGAAATGGTGGCTGGCCTCTCCAGCC
TGATGGAGCCCATCATCATCGTGTTCCTGGGTACCTTGATTGGCGGCATCGTGGTGTCAATGTACCTCCCCATTTTCAAA
TTGGGTCAGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.95

99.753

0.548
  pilG Neisseria gonorrhoeae MS11

52.645

98.025

0.516
  pilG Neisseria meningitidis 44/76-A

52.393

98.025

0.514
  pilC Legionella pneumophila strain ERS1305867

49.747

97.778

0.486
  pilC Acinetobacter baylyi ADP1

49.246

98.272

0.484
  pilC Acinetobacter baumannii D1279779

48.615

98.025

0.477
  pilC Vibrio cholerae strain A1552

40.351

98.519

0.398
  pilC Vibrio campbellii strain DS40M4

39.5

98.765

0.39
  pilC Thermus thermophilus HB27

37.221

99.506

0.37

Multiple sequence alignment