Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EAG14_RS17265 Genome accession   NZ_CP033069
Coordinates   3884174..3885907 (+) Length   577 a.a.
NCBI ID   WP_121729617.1    Uniprot ID   -
Organism   Acidovorax sp. 1608163     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3879174..3890907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAG14_RS17240 (EAG14_17240) proB 3879650..3880789 (-) 1140 WP_121729615.1 glutamate 5-kinase -
  EAG14_RS17245 (EAG14_17245) cgtA 3880857..3881927 (-) 1071 WP_121454524.1 Obg family GTPase CgtA -
  EAG14_RS17250 (EAG14_17250) rpmA 3882178..3882435 (-) 258 WP_026437555.1 50S ribosomal protein L27 -
  EAG14_RS17255 (EAG14_17255) rplU 3882451..3882762 (-) 312 WP_010462012.1 50S ribosomal protein L21 -
  EAG14_RS17260 (EAG14_17260) - 3883005..3883901 (+) 897 WP_240456818.1 polyprenyl synthetase family protein -
  EAG14_RS17265 (EAG14_17265) pilB 3884174..3885907 (+) 1734 WP_121729617.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EAG14_RS17270 (EAG14_17270) pilC 3885931..3887148 (+) 1218 WP_121729618.1 type II secretion system F family protein Machinery gene
  EAG14_RS17275 (EAG14_17275) pilD 3887148..3888020 (+) 873 WP_121729619.1 A24 family peptidase Machinery gene
  EAG14_RS17280 (EAG14_17280) coaE 3888023..3888637 (+) 615 WP_121729620.1 dephospho-CoA kinase -
  EAG14_RS17285 (EAG14_17285) zapD 3888727..3889482 (+) 756 WP_121729621.1 cell division protein ZapD -
  EAG14_RS17290 (EAG14_17290) - 3889515..3889748 (+) 234 WP_121456223.1 DNA gyrase inhibitor YacG -
  EAG14_RS17295 (EAG14_17295) - 3889816..3890223 (-) 408 WP_121729622.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62710.83 Da        Isoelectric Point: 5.0878

>NTDB_id=321149 EAG14_RS17265 WP_121729617.1 3884174..3885907(+) (pilB) [Acidovorax sp. 1608163]
MAAVDTAPKDASAIALPGLGRALMSAGKLAQKAAEEIYQKSQSNRTSFIAELTGSGVVSASDLAHTVSAVFGAPLLDLEA
IDPQRLPKDLLDAKICQSYKVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLNRLVEASTKSTTESM
ESLTSGEFDFDESVTEEVPESIDAGANEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRVDGELKEISSPPI
AIKDKLASRIKVISRMDISEKRVPQDGKMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLSAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAACKAPADVPH
EALVEAGYTEEELDGSWITYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQARNEGVRSLRASGLYK
AKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=321149 EAG14_RS17265 WP_121729617.1 3884174..3885907(+) (pilB) [Acidovorax sp. 1608163]
ATGGCTGCTGTTGACACCGCTCCTAAAGACGCTTCTGCAATTGCCCTTCCCGGGTTGGGGCGGGCGCTCATGTCTGCGGG
CAAGCTGGCACAAAAAGCCGCAGAGGAAATTTACCAAAAGTCACAAAGTAATCGCACCAGCTTTATTGCGGAACTGACTG
GCTCAGGTGTCGTTTCTGCGTCAGATCTGGCGCACACAGTTTCTGCAGTTTTTGGTGCCCCTTTGCTGGACTTGGAGGCC
ATTGACCCACAACGGCTCCCCAAAGATTTGCTTGATGCAAAAATCTGCCAGTCTTACAAGGTTGTCGTTCTCAGCAAGAG
AAACAACCGACTGATTGTCGCCACCGCAGACCCCACCGATCAGGAAGCAGCAGAAAAAATCAAGTTCACAACCCAAATGG
GAGTGGACTGGATCATTGCCGAATACGACAAGCTCAATCGCTTGGTCGAAGCGAGCACAAAGTCCACGACGGAGTCGATG
GAGAGCCTGACCAGCGGTGAATTCGATTTTGACGAATCGGTTACGGAGGAGGTTCCGGAATCCATCGATGCAGGCGCAAA
TGAGGTCGAAGATGCCCCTGTCGTTAAGTTCTTGCACAAAATGCTGCTTGATGCATTCAATATGCGGGCATCCGATTTGC
ATTTTGAACCCTACGAGCACCAGTACCGCGTACGCTTCCGTGTTGACGGGGAGCTCAAGGAAATTTCCTCGCCCCCCATT
GCCATCAAAGACAAACTCGCTTCCCGCATCAAGGTGATATCTCGCATGGATATCTCCGAAAAACGAGTACCTCAAGACGG
GAAAATGAAGCTCAAGGTGGGGCCGGACCGTGTGATTGACTTCCGGGTCAGCACTTTGCCCACTTTGTTTGGCGAGAAAA
TCGTGATCCGTATCCTGGACCCCAGCAGTGCCAAGCTGGGCATTGATGCGCTGGGCTATGAACCAGAGGAAAAAGAGCGT
CTCCTGTCTGCAATTGGTCGCCCTTATGGAATGATTTTGGTGACAGGCCCCACAGGTTCTGGAAAAACGGTGTCTCTGTA
TACCTGCCTGAACCTGCTCAACAAACCGGGCGTCAACATTGCGACGGCGGAAGACCCATCTGAAATTAATCTTCCAGGGG
TCAACCAAGTCAATGTGAACGACAAGGCAGGCCTTACTTTTGCGGTGGCCTTGAAGTCTTTCCTGCGTCAAGATCCGGAC
ATCATCATGGTGGGTGAAATTCGAGACCTGGAAACCGCTGATATTTCCATCAAAGCGGCCCAAACAGGTCACTTGGTTTT
ATCCACACTGCACACCAACGATGCCCCCACGACATTGACGCGGATGCGCAATATGGGCATTGCGCCTTTCAATATTGCAT
CGAGCGTGATTTTGATCACTGCCCAGCGCTTGGCCAGACGCCTGTGTGCAGCCTGCAAGGCCCCTGCGGATGTGCCCCAC
GAGGCCTTGGTGGAGGCCGGATACACCGAGGAAGAACTCGATGGATCTTGGATCACCTACAAACCCGTGGGCTGCTCCGC
GTGCAATAACGGATACAAAGGACGCGTGGGCATTTACCAAGTAATGCCCATCTCTGAGGAGATTCAACGCATTATCTTGC
GCGATGGAAGCGCCCTCGAAATTGCAGAACAAGCACGCAACGAGGGCGTTCGATCCTTGCGCGCATCAGGCCTCTACAAG
GCCAAACTCGGCCTCACTTCGCTGGAAGAAGTGCTCGCCGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.152

100

0.542
  pilB Acinetobacter baumannii D1279779

52.595

100

0.527
  pilF Neisseria gonorrhoeae MS11

53.369

97.747

0.522
  pilB Legionella pneumophila strain ERS1305867

48.94

98.094

0.48
  pilB Vibrio campbellii strain DS40M4

44.797

98.267

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.035

97.747

0.44
  pilB Vibrio cholerae strain A1552

47.217

90.295

0.426
  pilF Thermus thermophilus HB27

39.469

97.92

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.529

98.96

0.381

Multiple sequence alignment