Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CFK76_RS02370 Genome accession   NZ_CP032652
Coordinates   464352..465242 (+) Length   296 a.a.
NCBI ID   WP_025016380.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain LZ217     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459352..470242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK76_RS02335 (CFK76_02335) - 459787..460842 (+) 1056 WP_129584050.1 hypothetical protein -
  CFK76_RS02340 (CFK76_02340) - 460856..461644 (+) 789 WP_061827022.1 hypothetical protein -
  CFK76_RS02345 (CFK76_02345) - 461641..462255 (+) 615 WP_016265380.1 hypothetical protein -
  CFK76_RS02355 (CFK76_02355) - 462442..462816 (-) 375 WP_011375004.1 hypothetical protein -
  CFK76_RS02360 (CFK76_02360) - 462913..463413 (+) 501 WP_016265379.1 VanZ family protein -
  CFK76_RS02365 (CFK76_02365) - 463504..464235 (+) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CFK76_RS02370 (CFK76_02370) comGA 464352..465242 (+) 891 WP_025016380.1 competence type IV pilus ATPase ComGA Machinery gene
  CFK76_RS02375 (CFK76_02375) comGB 465235..466242 (+) 1008 WP_061827023.1 type II secretion system F family protein Machinery gene
  CFK76_RS02380 (CFK76_02380) comGC 466239..466538 (+) 300 WP_025016383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CFK76_RS02385 (CFK76_02385) comGD 466558..466962 (+) 405 WP_235596237.1 hypothetical protein Machinery gene
  CFK76_RS02390 (CFK76_02390) comGE 466949..467254 (+) 306 WP_016265375.1 hypothetical protein Machinery gene
  CFK76_RS02395 (CFK76_02395) comGF 467229..467741 (+) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFK76_RS02400 (CFK76_02400) - 467704..468042 (+) 339 WP_035145011.1 hypothetical protein -
  CFK76_RS02405 (CFK76_02405) - 468157..469176 (+) 1020 WP_235596233.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32872.67 Da        Isoelectric Point: 6.6910

>NTDB_id=317581 CFK76_RS02370 WP_025016380.1 464352..465242(+) (comGA) [Latilactobacillus sakei strain LZ217]
MSTEEKLTTLLKHCAKHHVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTRG
VVARLLDLGISNLQLRACLTGIAYQSLVTKDDIVQAHYELLTGSDYFSAKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=317581 CFK76_RS02370 WP_025016380.1 464352..465242(+) (comGA) [Latilactobacillus sakei strain LZ217]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAGCACCATGTGCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAACTGGCTGACCAAACAATTTATTTACGCCTAGCGACGGTTGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATTTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTGCGATTGCGTCCAGATACGCTGATTATCGGTGAGATTCGCGATTTAG
AGACGGCCCAATATGCCGTTTCCGCGGCTTTAAGTGGCCACCTGGTATTGAGCACAATCCATGCTCGAAGTACACGGGGC
GTGGTCGCGCGTTTATTGGATCTTGGTATTTCTAATCTGCAATTACGAGCCTGTCTAACGGGGATTGCCTATCAATCATT
GGTGACAAAAGACGACATTGTGCAAGCCCATTATGAGTTATTAACTGGTTCAGATTATTTCTCGGCAAAAGAAGGTGGAT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

97.297

100

0.973
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.279

91.892

0.389
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.912

91.892

0.385
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

39.929

95.608

0.382
  comGA Lactococcus lactis subsp. cremoris KW2

37.884

98.986

0.375
  comYA Streptococcus gordonii str. Challis substr. CH1

40.755

89.527

0.365
  comYA Streptococcus mutans UA140

38.909

92.905

0.361
  comYA Streptococcus mutans UA159

38.909

92.905

0.361

Multiple sequence alignment