Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CH576_RS03635 Genome accession   NZ_CP031691
Coordinates   719161..720555 (+) Length   464 a.a.
NCBI ID   WP_105894635.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P615-8618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714161..725555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH576_RS03610 (CH576_03610) rsmE 714179..714916 (-) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CH576_RS03615 (CH576_03615) lnt 714966..716495 (-) 1530 WP_116941799.1 apolipoprotein N-acyltransferase -
  CH576_RS03620 (CH576_03620) corC 716518..717417 (-) 900 WP_116941683.1 CNNM family magnesium/cobalt transport protein CorC -
  CH576_RS03625 (CH576_03625) ampD 718040..718600 (-) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH576_RS03630 (CH576_03630) pilA 718715..719164 (+) 450 WP_105894634.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH576_RS03635 (CH576_03635) pilB 719161..720555 (+) 1395 WP_105894635.1 GspE/PulE family protein Machinery gene
  CH576_RS03640 (CH576_03640) pilC 720552..721769 (+) 1218 WP_105894636.1 type II secretion system F family protein Machinery gene
  CH576_RS03645 (CH576_03645) pilD 721766..722458 (+) 693 WP_105894637.1 A24 family peptidase Machinery gene
  CH576_RS03650 (CH576_03650) rho 722512..723774 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  CH576_RS03655 (CH576_03655) metJ 724021..724338 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH576_RS03660 (CH576_03660) cueR 724352..724738 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  CH576_RS03665 (CH576_03665) - 724815..725021 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  CH576_RS03670 (CH576_03670) - 725096..725302 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52986.45 Da        Isoelectric Point: 5.6813

>NTDB_id=310239 CH576_RS03635 WP_105894635.1 719161..720555(+) (pilB) [Haemophilus influenzae strain P615-8618]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQYQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFQNLRVSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=310239 CH576_RS03635 WP_105894635.1 719161..720555(+) (pilB) [Haemophilus influenzae strain P615-8618]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCTGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACTATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAATATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACACATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACAGATTTTCAAAATTTACGTGTAAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.707

100

0.987
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.489

97.845

0.563
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.422
  pilB Legionella pneumophila strain ERS1305867

39.554

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.877

100

0.403
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment