Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CH576_RS03645 Genome accession   NZ_CP031691
Coordinates   721766..722458 (+) Length   230 a.a.
NCBI ID   WP_105894637.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P615-8618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716766..727458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH576_RS03625 (CH576_03625) ampD 718040..718600 (-) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH576_RS03630 (CH576_03630) pilA 718715..719164 (+) 450 WP_105894634.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH576_RS03635 (CH576_03635) pilB 719161..720555 (+) 1395 WP_105894635.1 GspE/PulE family protein Machinery gene
  CH576_RS03640 (CH576_03640) pilC 720552..721769 (+) 1218 WP_105894636.1 type II secretion system F family protein Machinery gene
  CH576_RS03645 (CH576_03645) pilD 721766..722458 (+) 693 WP_105894637.1 A24 family peptidase Machinery gene
  CH576_RS03650 (CH576_03650) rho 722512..723774 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  CH576_RS03655 (CH576_03655) metJ 724021..724338 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH576_RS03660 (CH576_03660) cueR 724352..724738 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  CH576_RS03665 (CH576_03665) - 724815..725021 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  CH576_RS03670 (CH576_03670) - 725096..725302 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  CH576_RS03675 (CH576_03675) - 725377..725583 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26760.76 Da        Isoelectric Point: 9.2892

>NTDB_id=310241 CH576_RS03645 WP_105894637.1 721766..722458(+) (pilD) [Haemophilus influenzae strain P615-8618]
MIYFTMFLLGGILGIALWFYLSGFITRLQQNIYAIYVELFPQNRSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWLAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=310241 CH576_RS03645 WP_105894637.1 721766..722458(+) (pilD) [Haemophilus influenzae strain P615-8618]
ATGATTTATTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCG
TTTGCAGCAAAATATTTATGCGATTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTACTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGCTTGCAAAATTCTATTATGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAGGAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

99.565

100

0.996
  pilD Haemophilus influenzae 86-028NP

95.217

100

0.952

Multiple sequence alignment