Detailed information    

experimental Experimentally validated

Overview

Name   mutX   Type   Machinery gene
Locus tag   SPR_RS05270 Genome accession   NC_003098
Coordinates   1050341..1050805 (-) Length   154 a.a.
NCBI ID   WP_001135782.1    Uniprot ID   P59659
Organism   Streptococcus pneumoniae R6     
Function   DNA mismatch repair   
Homologous recombination

Function

Generation of a mutator phenotype in S. pneumoniae through deletions of mutX, hexA or hexB enhanced the expression of competence.


Genomic Context

Location: 1045341..1055805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS05250 (spr1051) - 1046292..1047260 (-) 969 WP_000105362.1 thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha -
  SPR_RS05255 (spr1052) pdrM 1047414..1048775 (-) 1362 WP_000278532.1 sodium-coupled multidrug efflux MATE transporter PdrM -
  SPR_RS05260 - 1048786..1049046 (-) 261 WP_001105925.1 hypothetical protein -
  SPR_RS05265 (spr1053) - 1049060..1050328 (-) 1269 WP_000924481.1 dihydroorotase -
  SPR_RS05270 (spr1054) mutX 1050341..1050805 (-) 465 WP_001135782.1 NUDIX hydrolase Machinery gene
  SPR_RS05275 (spr1055) - 1050815..1051468 (-) 654 WP_000401328.1 uracil-DNA glycosylase -
  SPR_RS05280 (spr1056) - 1051604..1052251 (-) 648 WP_001824648.1 hypothetical protein -
  SPR_RS05285 (spr1057) - 1052265..1052978 (-) 714 WP_000499433.1 YjjG family noncanonical pyrimidine nucleotidase -
  SPR_RS05290 (spr1058) dhaM 1053436..1053816 (-) 381 Protein_1045 dihydroxyacetone kinase phosphoryl donor subunit DhaM -
  SPR_RS11095 - 1053816..1053890 (-) 75 Protein_1046 dihydroxyacetone kinase subunit L -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 17870.30 Da        Isoelectric Point: 4.2287

>NTDB_id=660 SPR_RS05270 WP_001135782.1 1050341..1050805(-) (mutX) [Streptococcus pneumoniae R6]
MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGETPQECAVREILEETGLKAKPVLKGVITFPEFTPDLD
WYTYVFKVTEFEGDLIDCNEGMLEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=660 SPR_RS05270 WP_001135782.1 1050341..1050805(-) (mutX) [Streptococcus pneumoniae R6]
ATGCCTCAGTTAGCGACGATTTGTTACATTGATAATGGGAAAGAACTGCTCATGCTCCATCGTAATAAGAAACCCAATGA
TGTCCATGAAGGGAAATGGATTGGTGTGGGTGGTAAGCTAGAGAGAGGAGAGACGCCCCAGGAATGCGCGGTACGTGAAA
TCCTTGAAGAAACAGGGCTCAAAGCCAAGCCAGTTCTAAAAGGTGTCATCACTTTTCCTGAATTTACACCAGATTTAGAC
TGGTACACCTATGTTTTTAAGGTGACGGAGTTTGAGGGCGACTTGATTGACTGCAATGAGGGGATGCTAGAATGGGTTCC
CTATGATGAGGTTTTGAGCAAGCCGACCTGGGAAGGTGACCACACCTTTGTTGAGTGGCTTTTAGAGGATAAACCCTTCT
TTTCAGCCAAGTTTGTTTATGATGGGGATAAATTGTTGGATACCCAAGTTGATTTCTATGAATAA

Domains

Predicted by InterProScan.

NUDIX domain (NUDIX)

(2-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P59659

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Alyssa L Gagne et al. (2013) Competence in Streptococcus pneumoniae is a response to an increasing mutational burden. PloS One 8(8):e72613. [PMID: 23967325]