Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DY328_RS05235 Genome accession   NZ_CP031556
Coordinates   982155..983036 (+) Length   293 a.a.
NCBI ID   WP_257151376.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain Sag27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977155..988036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DY328_RS05220 (DY328_05220) rpoC 977853..981503 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  DY328_RS05225 (DY328_05225) - 981617..981982 (+) 366 WP_000285373.1 DUF1033 family protein -
  DY328_RS05235 (DY328_05235) comYA 982155..983036 (+) 882 WP_257151376.1 competence type IV pilus ATPase ComGA Machinery gene
  DY328_RS05240 (DY328_05240) comYB 983033..984076 (+) 1044 WP_228308067.1 competence type IV pilus assembly protein ComGB Machinery gene
  DY328_RS05245 (DY328_05245) comGF 984073..984528 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF -
  DY328_RS05250 (DY328_05250) comGG 984506..984877 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  DY328_RS05255 (DY328_05255) comYH 984992..985966 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  DY328_RS05260 (DY328_05260) - 985998..987191 (+) 1194 WP_000047534.1 acetate kinase -
  DY328_RS05265 (DY328_05265) - 987343..987549 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  DY328_RS05270 (DY328_05270) - 987608..987745 (+) 138 WP_001865900.1 hypothetical protein -

Sequence

Protein


Download         Length: 293 a.a.        Molecular weight: 33493.62 Da        Isoelectric Point: 7.4269

>NTDB_id=307583 DY328_RS05235 WP_257151376.1 982155..983036(+) (comYA) [Streptococcus agalactiae strain Sag27]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGIRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVE

Nucleotide


Download         Length: 882 bp        

>NTDB_id=307583 DY328_RS05235 WP_257151376.1 982155..983036(+) (comYA) [Streptococcus agalactiae strain Sag27]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTATAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGAAT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

70.89

99.659

0.706
  comYA Streptococcus mutans UA140

70.89

99.659

0.706
  comYA Streptococcus gordonii str. Challis substr. CH1

65.188

100

0.652
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

63.14

100

0.631
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.014

99.659

0.628
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

62.116

100

0.621
  comGA/cglA/cilD Streptococcus pneumoniae D39

62.116

100

0.621
  comGA/cglA/cilD Streptococcus pneumoniae R6

62.116

100

0.621
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

62.116

100

0.621
  comGA Lactococcus lactis subsp. cremoris KW2

53.242

100

0.532
  comGA Latilactobacillus sakei subsp. sakei 23K

39.925

91.468

0.365
  comGA Bacillus subtilis subsp. subtilis str. 168

37.589

96.246

0.362

Multiple sequence alignment