Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DY328_RS05240 Genome accession   NZ_CP031556
Coordinates   983033..984076 (+) Length   347 a.a.
NCBI ID   WP_228308067.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain Sag27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 978033..989076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DY328_RS05225 (DY328_05225) - 981617..981982 (+) 366 WP_000285373.1 DUF1033 family protein -
  DY328_RS05235 (DY328_05235) comYA 982155..983036 (+) 882 WP_257151376.1 competence type IV pilus ATPase ComGA Machinery gene
  DY328_RS05240 (DY328_05240) comYB 983033..984076 (+) 1044 WP_228308067.1 competence type IV pilus assembly protein ComGB Machinery gene
  DY328_RS05245 (DY328_05245) comGF 984073..984528 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF -
  DY328_RS05250 (DY328_05250) comGG 984506..984877 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  DY328_RS05255 (DY328_05255) comYH 984992..985966 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  DY328_RS05260 (DY328_05260) - 985998..987191 (+) 1194 WP_000047534.1 acetate kinase -
  DY328_RS05265 (DY328_05265) - 987343..987549 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  DY328_RS05270 (DY328_05270) - 987608..987745 (+) 138 WP_001865900.1 hypothetical protein -
  DY328_RS05275 (DY328_05275) - 987786..988241 (+) 456 WP_000905673.1 hypothetical protein -
  DY328_RS05280 (DY328_05280) - 988310..988975 (+) 666 WP_000008113.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39964.70 Da        Isoelectric Point: 9.9002

>NTDB_id=307584 DY328_RS05240 WP_228308067.1 983033..984076(+) (comYB) [Streptococcus agalactiae strain Sag27]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTVYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=307584 DY328_RS05240 WP_228308067.1 983033..984076(+) (comYB) [Streptococcus agalactiae strain Sag27]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGTTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

61.877

98.271

0.608
  comYB Streptococcus mutans UA159

61.584

98.271

0.605
  comYB Streptococcus gordonii str. Challis substr. CH1

56.012

98.271

0.55
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484

Multiple sequence alignment