Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV428_RS03640 Genome accession   NZ_CP031243
Coordinates   702916..704310 (+) Length   464 a.a.
NCBI ID   WP_114908722.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M19346     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697916..709310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV428_RS03610 - 698211..699062 (-) 852 WP_114908717.1 thymidylate synthase -
  DV428_RS03615 lgt 699072..699878 (-) 807 WP_114908718.1 prolipoprotein diacylglyceryl transferase -
  DV428_RS03620 - 699887..700684 (-) 798 WP_114908719.1 sulfite exporter TauE/SafE family protein -
  DV428_RS03625 rppH 700684..701274 (-) 591 WP_111696429.1 RNA pyrophosphohydrolase -
  DV428_RS03630 ampD 701801..702355 (-) 555 WP_114908720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV428_RS03635 pilA 702470..702919 (+) 450 WP_114908721.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV428_RS03640 pilB 702916..704310 (+) 1395 WP_114908722.1 GspE/PulE family protein Machinery gene
  DV428_RS03645 pilC 704307..705527 (+) 1221 WP_114908723.1 type II secretion system F family protein Machinery gene
  DV428_RS03650 pilD 705524..706216 (+) 693 WP_114908724.1 A24 family peptidase Machinery gene
  DV428_RS03655 rho 706271..707533 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  DV428_RS03660 metJ 707779..708096 (+) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DV428_RS03665 - 708110..708496 (-) 387 WP_111696423.1 Cu(I)-responsive transcriptional regulator -
  DV428_RS03670 - 708573..708779 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  DV428_RS03675 - 708864..709070 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  DV428_RS03680 - 709045..709131 (+) 87 Protein_699 heavy metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52797.17 Da        Isoelectric Point: 5.9136

>NTDB_id=304570 DV428_RS03640 WP_114908722.1 702916..704310(+) (pilB) [Haemophilus haemolyticus strain M19346]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQNNASDIHLETLSDHFQVRFRIDGVL
QAQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNIPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=304570 DV428_RS03640 WP_114908722.1 702916..704310(+) (pilB) [Haemophilus haemolyticus strain M19346]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGAGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAACAATGCCTCAGATATTCATTTGGAGACCTTATCGGATCATTTCCAAGTACGGTTTAGAATTGACGGTGTTTTA
CAAGCTCAGCCTCCACTTAGCAAAAATTTAGCGAATCGAATTATTTCTCGCCTAAAACTGCTCGCTAAATTAGATATCAG
CGAAACCCGCCTTCCACAAGATGGACGATTTCAATTTAAAACAACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACGT
TACCAACTCATTGGGGAGAGAAAGTTGTCCTGAGAGCACAGCAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTGAATATACCAGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAAATAGGACTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCCGACATCATTATGCTGGGTGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACTGGGCATTTAGTGCTTTCAACTTTACATACTAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAGCATGAAATTGAAAACAGTTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

95.69

100

0.957
  pilB Haemophilus influenzae Rd KW20

94.816

99.784

0.946
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.122

100

0.442
  pilB Legionella pneumophila strain ERS1305867

40.404

100

0.431
  pilB Vibrio campbellii strain DS40M4

40.452

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.418
  pilB Acinetobacter baylyi ADP1

38.554

100

0.414
  pilB Acinetobacter baumannii D1279779

39.337

100

0.409
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375
  pilF Thermus thermophilus HB27

35.714

100

0.366

Multiple sequence alignment