Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV428_RS03635 Genome accession   NZ_CP031243
Coordinates   702470..702919 (+) Length   149 a.a.
NCBI ID   WP_114908721.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M19346     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697470..707919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV428_RS03605 tadA 697696..698211 (-) 516 WP_114908716.1 tRNA adenosine(34) deaminase TadA -
  DV428_RS03610 - 698211..699062 (-) 852 WP_114908717.1 thymidylate synthase -
  DV428_RS03615 lgt 699072..699878 (-) 807 WP_114908718.1 prolipoprotein diacylglyceryl transferase -
  DV428_RS03620 - 699887..700684 (-) 798 WP_114908719.1 sulfite exporter TauE/SafE family protein -
  DV428_RS03625 rppH 700684..701274 (-) 591 WP_111696429.1 RNA pyrophosphohydrolase -
  DV428_RS03630 ampD 701801..702355 (-) 555 WP_114908720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV428_RS03635 pilA 702470..702919 (+) 450 WP_114908721.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV428_RS03640 pilB 702916..704310 (+) 1395 WP_114908722.1 GspE/PulE family protein Machinery gene
  DV428_RS03645 pilC 704307..705527 (+) 1221 WP_114908723.1 type II secretion system F family protein Machinery gene
  DV428_RS03650 pilD 705524..706216 (+) 693 WP_114908724.1 A24 family peptidase Machinery gene
  DV428_RS03655 rho 706271..707533 (-) 1263 WP_005629550.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15454.80 Da        Isoelectric Point: 8.4623

>NTDB_id=304569 DV428_RS03635 WP_114908721.1 702470..702919(+) (pilA) [Haemophilus haemolyticus strain M19346]
MKLTTQTTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQATGNAAAGVTWTTTCKGTDASLFPANFCGSVAQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=304569 DV428_RS03635 WP_114908721.1 702470..702919(+) (pilA) [Haemophilus haemolyticus strain M19346]
ATGAAACTAACTACACAAACTACCTTGAAAAAAGGCTTTACCTTAATTGAATTGATGATAGTCATCGCCATCATCGCGAT
TTTAGCCACTATCGCTATTCCTTCTTATCAAAATTATACCAAAAAAGCAGCGGTATCCGAATTACTGCAAGCTTCTGCAC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACAGGGGGAAAAAATGGTATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCGGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCGCACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

94.631

100

0.946
  pilA Haemophilus influenzae 86-028NP

93.96

100

0.94
  pilA Glaesserella parasuis strain SC1401

53.947

100

0.55
  pilA2 Legionella pneumophila strain ERS1305867

40.136

98.658

0.396
  pilA Vibrio campbellii strain DS40M4

40.426

94.631

0.383
  pilA2 Legionella pneumophila str. Paris

40.288

93.289

0.376

Multiple sequence alignment