Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV384_RS05350 Genome accession   NZ_CP031241
Coordinates   1103336..1104730 (-) Length   464 a.a.
NCBI ID   WP_114890120.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M17648     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098336..1109730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV384_RS05315 - 1098605..1098811 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  DV384_RS05320 - 1098885..1099091 (-) 207 WP_015701556.1 heavy-metal-associated domain-containing protein -
  DV384_RS05325 - 1099168..1099545 (+) 378 WP_075913212.1 Cu(I)-responsive transcriptional regulator -
  DV384_RS05330 metJ 1099559..1099876 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DV384_RS05335 rho 1100112..1101374 (+) 1263 WP_114890123.1 transcription termination factor Rho -
  DV384_RS05340 pilD 1101430..1102125 (-) 696 WP_114890122.1 A24 family peptidase Machinery gene
  DV384_RS05345 pilC 1102122..1103339 (-) 1218 WP_114890121.1 type II secretion system F family protein Machinery gene
  DV384_RS05350 pilB 1103336..1104730 (-) 1395 WP_114890120.1 GspE/PulE family protein Machinery gene
  DV384_RS05355 pilA 1104727..1105176 (-) 450 WP_114934572.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV384_RS05360 ampD 1105291..1105851 (+) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV384_RS05365 corC 1106475..1107374 (+) 900 WP_114934573.1 CNNM family magnesium/cobalt transport protein CorC -
  DV384_RS05370 lnt 1107397..1108926 (+) 1530 WP_114890118.1 apolipoprotein N-acyltransferase -
  DV384_RS05375 rsmE 1108978..1109715 (+) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52969.40 Da        Isoelectric Point: 5.6399

>NTDB_id=304494 DV384_RS05350 WP_114890120.1 1103336..1104730(-) (pilB) [Haemophilus influenzae strain M17648]
MTSYALLHTQRVTAQNGEVFTISPDLWARNQQQHSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQHLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTYWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=304494 DV384_RS05350 WP_114890120.1 1103336..1104730(-) (pilB) [Haemophilus influenzae strain M17648]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGGTATTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCACTCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACACTTAATAAGCAAAATATTCGCTAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCTATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.06

100

0.981
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.675

98.276

0.567
  pilB Vibrio cholerae strain A1552

39.771

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.574

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.289

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.831

100

0.401
  pilF Thermus thermophilus HB27

36.701

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.611

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377

Multiple sequence alignment