Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV384_RS05355 Genome accession   NZ_CP031241
Coordinates   1104727..1105176 (-) Length   149 a.a.
NCBI ID   WP_114934572.1    Uniprot ID   -
Organism   Haemophilus influenzae strain M17648     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1099727..1110176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV384_RS05335 rho 1100112..1101374 (+) 1263 WP_114890123.1 transcription termination factor Rho -
  DV384_RS05340 pilD 1101430..1102125 (-) 696 WP_114890122.1 A24 family peptidase Machinery gene
  DV384_RS05345 pilC 1102122..1103339 (-) 1218 WP_114890121.1 type II secretion system F family protein Machinery gene
  DV384_RS05350 pilB 1103336..1104730 (-) 1395 WP_114890120.1 GspE/PulE family protein Machinery gene
  DV384_RS05355 pilA 1104727..1105176 (-) 450 WP_114934572.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV384_RS05360 ampD 1105291..1105851 (+) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV384_RS05365 corC 1106475..1107374 (+) 900 WP_114934573.1 CNNM family magnesium/cobalt transport protein CorC -
  DV384_RS05370 lnt 1107397..1108926 (+) 1530 WP_114890118.1 apolipoprotein N-acyltransferase -
  DV384_RS05375 rsmE 1108978..1109715 (+) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15321.69 Da        Isoelectric Point: 8.7960

>NTDB_id=304495 DV384_RS05355 WP_114934572.1 1104727..1105176(-) (pilA) [Haemophilus influenzae strain M17648]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANMEYILQASGNAAAGVTWTTTCKGTDASLFPANFCGSVAQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=304495 DV384_RS05355 WP_114934572.1 1104727..1105176(-) (pilA) [Haemophilus influenzae strain M17648]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCAC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTAATCACTCAATCAGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCCAGTGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCGCACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

93.289

100

0.933
  pilA Haemophilus influenzae 86-028NP

88.591

100

0.886
  pilA Glaesserella parasuis strain SC1401

55.921

100

0.57
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383
  pilA2 Legionella pneumophila str. Paris

42.748

87.919

0.376
  pilA Vibrio campbellii strain DS40M4

38.298

94.631

0.362

Multiple sequence alignment