Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV441_RS05980 Genome accession   NZ_CP031238
Coordinates   1237955..1238395 (-) Length   146 a.a.
NCBI ID   WP_005627463.1    Uniprot ID   A0A0M3G8H8
Organism   Haemophilus haemolyticus strain M28486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232955..1243395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV441_RS05960 rho 1233342..1234604 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV441_RS05965 pilD 1234658..1235350 (-) 693 WP_046952737.1 prepilin peptidase Machinery gene
  DV441_RS05970 pilC 1235347..1236567 (-) 1221 WP_046952736.1 type II secretion system F family protein Machinery gene
  DV441_RS05975 pilB 1236564..1237958 (-) 1395 WP_114892533.1 GspE/PulE family protein Machinery gene
  DV441_RS05980 pilA 1237955..1238395 (-) 441 WP_005627463.1 prepilin peptidase-dependent pilin Machinery gene
  DV441_RS05985 ampD 1238510..1239064 (+) 555 WP_005627465.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV441_RS05990 rppH 1239590..1240180 (+) 591 WP_005627467.1 RNA pyrophosphohydrolase -
  DV441_RS05995 - 1240180..1240977 (+) 798 WP_005627469.1 sulfite exporter TauE/SafE family protein -
  DV441_RS06000 lgt 1240986..1241792 (+) 807 WP_046952735.1 prolipoprotein diacylglyceryl transferase -
  DV441_RS06005 - 1241803..1242654 (+) 852 WP_005627473.1 thymidylate synthase -
  DV441_RS06010 tadA 1242654..1243169 (+) 516 WP_005627475.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15050.28 Da        Isoelectric Point: 8.4607

>NTDB_id=304405 DV441_RS05980 WP_005627463.1 1237955..1238395(-) (pilA) [Haemophilus haemolyticus strain M28486]
MKLTTQTTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVAGNGTLDGMSYTLTAEGDSAKGVTWKTTCGTTNADIFPAGFCSK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=304405 DV441_RS05980 WP_005627463.1 1237955..1238395(-) (pilA) [Haemophilus haemolyticus strain M28486]
ATGAAACTAACTACACAAACTACCTTGAAAAAAGGGTTTACCTTAATTGAATTAATGATAGTCATCGCTATCATTGCGAT
TTTAGCTACTATCGCTATTCCTTCTTATCAAAATTATACCAAAAAAGCAGCGGTATCCGAATTACTGCAAGCTTCCGCAC
CTTATAAGTCAGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTACTTGCTCGGGAGGAAGCAATGGAATTGCG
GCTGATATTACAACTGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGCGGGTAATGGTAC
ATTAGATGGAATGAGTTACACACTCACAGCTGAAGGAGATAGTGCAAAAGGCGTTACATGGAAAACCACCTGTGGCACAA
CGAATGCCGATATTTTCCCTGCAGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M3G8H8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

81.25

98.63

0.801
  pilA Haemophilus influenzae Rd KW20

77.083

98.63

0.76
  pilA Glaesserella parasuis strain SC1401

55.333

100

0.568
  pilA2 Legionella pneumophila str. Paris

42.177

100

0.425
  pilA2 Legionella pneumophila strain ERS1305867

42.177

100

0.425
  comP Acinetobacter baylyi ADP1

41.379

99.315

0.411
  pilA Vibrio campbellii strain DS40M4

36.986

100

0.37
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.063

97.945

0.363

Multiple sequence alignment