Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV441_RS05975 Genome accession   NZ_CP031238
Coordinates   1236564..1237958 (-) Length   464 a.a.
NCBI ID   WP_114892533.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M28486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1231564..1242958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV441_RS05945 - 1232094..1232300 (-) 207 WP_046952739.1 heavy-metal-associated domain-containing protein -
  DV441_RS05950 cueR 1232377..1232763 (+) 387 WP_046952738.1 Cu(I)-responsive transcriptional regulator -
  DV441_RS05955 metJ 1232777..1233094 (-) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DV441_RS05960 rho 1233342..1234604 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV441_RS05965 pilD 1234658..1235350 (-) 693 WP_046952737.1 prepilin peptidase Machinery gene
  DV441_RS05970 pilC 1235347..1236567 (-) 1221 WP_046952736.1 type II secretion system F family protein Machinery gene
  DV441_RS05975 pilB 1236564..1237958 (-) 1395 WP_114892533.1 GspE/PulE family protein Machinery gene
  DV441_RS05980 pilA 1237955..1238395 (-) 441 WP_005627463.1 prepilin peptidase-dependent pilin Machinery gene
  DV441_RS05985 ampD 1238510..1239064 (+) 555 WP_005627465.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV441_RS05990 rppH 1239590..1240180 (+) 591 WP_005627467.1 RNA pyrophosphohydrolase -
  DV441_RS05995 - 1240180..1240977 (+) 798 WP_005627469.1 sulfite exporter TauE/SafE family protein -
  DV441_RS06000 lgt 1240986..1241792 (+) 807 WP_046952735.1 prolipoprotein diacylglyceryl transferase -
  DV441_RS06005 - 1241803..1242654 (+) 852 WP_005627473.1 thymidylate synthase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52719.17 Da        Isoelectric Point: 6.4351

>NTDB_id=304404 DV441_RS05975 WP_114892533.1 1236564..1237958(-) (pilB) [Haemophilus haemolyticus strain M28486]
MIDLTSAKPRVKAQNGEIFTISPELWGRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKLVEPVLL
ESTQLKELLQQLAPNQPKVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQNNASDIHLETLSDHFQVRFRIDGVL
QAQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRTQQNKPVELSFSELG
MTESQQKEFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELNGIIQSQINPQIGLDFNRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYQGRIGVYQFLHWQQNGYQTDFKNLRASGLEKVRQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=304404 DV441_RS05975 WP_114892533.1 1236564..1237958(-) (pilB) [Haemophilus haemolyticus strain M28486]
ATGATCGACTTAACTAGTGCAAAACCTCGTGTAAAAGCACAAAATGGCGAGATATTTACGATCTCGCCAGAGTTATGGGG
GCGTAATCAGCAACAACAGTCGTTACTCTTACGTTATTTTGCTTTACCACTTAAAGAAGAAAATAATCGTCTTTGGCTAG
GTGTCGATTCTCTTTCAAATCTTTCCGCCTGTGAAACTATTGCGTTTATAACTGGCAAGCTTGTTGAGCCAGTTTTATTA
GAAAGCACTCAACTTAAAGAACTATTACAGCAACTTGCCCCTAATCAACCTAAAGTGGAAGAGCAAGTTAAATATTACCA
ACATCAAGAACAGTCTCATCTTGAACAACAAGATGACGAGCCCGTCATTCGCTTACTTAATCAAATTTTTGAATCCGCTT
TACAAAATAATGCCTCAGATATTCACTTGGAGACTTTATCGGATCATTTCCAAGTGCGGTTTAGAATTGACGGTGTTTTA
CAAGCTCAGCCTCCACTTAGTAAAAATTTGGCTAATCGAATTATTTCTCGCCTAAAACTGCTCGCTAAATTAGATATCAG
TGAAACCCGCCTTCCACAAGATGGACGATTTCAATTTAAAACTACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACGT
TACCAACTCATTGGGGAGAGAAAGTTGTCCTGAGAACACAGCAAAATAAACCTGTAGAACTCAGCTTTTCTGAACTAGGC
ATGACTGAAAGTCAACAGAAAGAATTTCAACGCGCACTTAGCCAACCCCAAGGATTAATTTTAGTAACCGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTAATGGAATTATTCAAAGCCAAATAAATCCACAGATTGGATTAGATTTTAACCGCCTATTGCGT
GCTTTCTTACGCCAAGATCCAGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCAATGATCGCGCTACGTGC
TGCCCAAACGGGGCATTTGGTACTCTCAACTTTACATACCAATGATGCAATATCTGCCATTTCCCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTGCTGCTCGTCATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACCGATTTTAAAAATTTACGTGCGAGTGGTTTAGAAAAAGTTCGCCAAGGTATAACGGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

91.648

98.06

0.899
  pilB Haemophilus influenzae Rd KW20

90.969

97.845

0.89
  pilB Glaesserella parasuis strain SC1401

58.553

98.276

0.575
  pilB Vibrio cholerae strain A1552

41.856

100

0.438
  pilB Vibrio campbellii strain DS40M4

40.862

100

0.429
  pilB Legionella pneumophila strain ERS1305867

39.596

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.42
  pilB Acinetobacter baumannii D1279779

39.545

100

0.412
  pilB Acinetobacter baylyi ADP1

38.277

100

0.412
  pilF Neisseria gonorrhoeae MS11

37.815

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.527

100

0.379
  pilF Thermus thermophilus HB27

36

100

0.369

Multiple sequence alignment