Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   DV441_RS05965 Genome accession   NZ_CP031238
Coordinates   1234658..1235350 (-) Length   230 a.a.
NCBI ID   WP_046952737.1    Uniprot ID   A0A0M3G8P8
Organism   Haemophilus haemolyticus strain M28486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1229658..1240350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV441_RS05940 - 1229948..1232119 (-) 2172 WP_046952740.1 heavy metal translocating P-type ATPase -
  DV441_RS05945 - 1232094..1232300 (-) 207 WP_046952739.1 heavy-metal-associated domain-containing protein -
  DV441_RS05950 cueR 1232377..1232763 (+) 387 WP_046952738.1 Cu(I)-responsive transcriptional regulator -
  DV441_RS05955 metJ 1232777..1233094 (-) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DV441_RS05960 rho 1233342..1234604 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV441_RS05965 pilD 1234658..1235350 (-) 693 WP_046952737.1 prepilin peptidase Machinery gene
  DV441_RS05970 pilC 1235347..1236567 (-) 1221 WP_046952736.1 type II secretion system F family protein Machinery gene
  DV441_RS05975 pilB 1236564..1237958 (-) 1395 WP_114892533.1 GspE/PulE family protein Machinery gene
  DV441_RS05980 pilA 1237955..1238395 (-) 441 WP_005627463.1 prepilin peptidase-dependent pilin Machinery gene
  DV441_RS05985 ampD 1238510..1239064 (+) 555 WP_005627465.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV441_RS05990 rppH 1239590..1240180 (+) 591 WP_005627467.1 RNA pyrophosphohydrolase -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26888.94 Da        Isoelectric Point: 9.2891

>NTDB_id=304402 DV441_RS05965 WP_046952737.1 1234658..1235350(-) (pilD) [Haemophilus haemolyticus strain M28486]
MIYFAMFLLGGILGIALWFYLSGFITRLQQEIYVTYVELFPQNRSPFQPHFVSIQQKKCGHILRYFLSIGVGFIFLQIAF
KDSIFTVWFGLTLLILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYR
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=304402 DV441_RS05965 WP_046952737.1 1234658..1235350(-) (pilD) [Haemophilus haemolyticus strain M28486]
ATGATTTACTTCGCAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCG
CTTGCAGCAAGAGATTTATGTGACTTACGTTGAATTATTTCCACAAAATCGTTCTCCATTTCAACCGCATTTTGTCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGCATTGGAGTAGGATTTATATTTTTACAAATTGCCTTT
AAAGATTCTATTTTTACTGTATGGTTCGGACTCACACTTCTTATTCTTTGGACAATTAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACCCTGTTTATGGTTACTTACGCTCGGTTTATTTGGAGCAGATAATAACTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACTTTACCGCACTT
TTTGTTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAGGAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCGGCCGTCATAATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M3G8P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

95.217

100

0.952
  pilD Haemophilus influenzae 86-028NP

94.348

100

0.943

Multiple sequence alignment