Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DS737_RS11660 Genome accession   NZ_CP030880
Coordinates   2554101..2555219 (+) Length   372 a.a.
NCBI ID   WP_005081584.1    Uniprot ID   N9GMD9
Organism   Acinetobacter haemolyticus strain HW-2A     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549101..2560219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS737_RS11640 - 2551194..2551466 (+) 273 WP_026070408.1 hypothetical protein -
  DS737_RS11645 - 2551538..2552209 (+) 672 WP_008940858.1 HAD family hydrolase -
  DS737_RS11650 - 2552216..2552908 (-) 693 WP_113997596.1 YggS family pyridoxal phosphate-dependent enzyme -
  DS737_RS11655 pilT 2553035..2554072 (+) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  DS737_RS11660 pilU 2554101..2555219 (+) 1119 WP_005081584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DS737_RS11665 fur 2555307..2555744 (-) 438 WP_004638213.1 ferric iron uptake transcriptional regulator -
  DS737_RS11670 - 2555856..2556254 (+) 399 WP_004638212.1 outer membrane protein assembly factor BamE -
  DS737_RS11675 - 2556311..2556631 (-) 321 WP_113997597.1 RnfH family protein -
  DS737_RS11680 - 2556631..2557713 (-) 1083 WP_113997598.1 hypothetical protein -
  DS737_RS11685 - 2557963..2558418 (-) 456 WP_008940857.1 bacteriohemerythrin -
  DS737_RS11690 - 2558693..2559541 (-) 849 WP_113997599.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41758.89 Da        Isoelectric Point: 6.4343

>NTDB_id=302026 DS737_RS11660 WP_005081584.1 2554101..2555219(+) (pilU) [Acinetobacter haemolyticus strain HW-2A]
MDFNDLLNLMIQQNASDLFITADVEPSMKINGQIVPVSKTKLSAEIVGQLVNSIMSDKQRKEFADTRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNHNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=302026 DS737_RS11660 WP_005081584.1 2554101..2555219(+) (pilU) [Acinetobacter haemolyticus strain HW-2A]
ATGGATTTTAATGATTTACTCAATTTGATGATTCAACAAAACGCATCTGATTTATTTATCACTGCTGATGTTGAACCATC
TATGAAAATTAACGGGCAAATTGTGCCTGTTTCTAAAACCAAATTATCTGCTGAAATTGTGGGGCAACTTGTCAATTCAA
TTATGAGTGACAAGCAACGTAAAGAATTTGCAGATACACGTGAGTGTAACTTCGCAATTACTAACCGTGAAAAAACTGCG
CGTTTTCGTGTCAGTGCCTTTCAGCAACGCGATATGCCTGGGATGATTTTACGTAAAATCGAAACCCATATTCCGAGTAT
GGACGATTTAAAACTGCCTGAAGTACTCAAAGAACTTGCCATGACCAAACGTGGCATTATTATCTTTGTTGGCGCAACAG
GTACAGGTAAATCTACTTCTCTCGCTTCAATGATTGGTTATCGTAATCACAATTCCAAAGGACACATTATCACCATCGAA
GATCCGATTGAATTTGTGCATCAACATGCGGGGTGTATCGTCACTCAACGTGAAGTCGGGATTGATACTGAATCTTTTGA
AGTTGCATTAAAAAATACACTTCGTCAGGCGCCAGATGTTATTTTGATTGGTGAGATTCGTTCACGCGAAACCATGGACT
ATGCGATCGCCTTTGCTGAAACAGGTCACTTGGTCTTGGCAACGCTTCACGCCAACAATGCCAACCAAGCCCTAGATCGT
ATTATTCACTTCTTCGAAGCCGATCGTCATAGCCAGTTGTATATGGATCTGTCACTTAACTTAAAGGCATTGGTCGCGCA
ACAATTGATCCCAACACCTGATGGAAATTCACGTCGTGCTGCGATTGAAATTTTGATCAATACTCCTTTATTGGCTGACT
ATATTCGCAAAGGTGAAATTCATGAGATCAAAGATCTGATGAAACGCTCTCGTGAATTGGGTATGCAAACCTTTGACCAA
GCCCTATTTGACCTCTATAAAGCGGGACAAATCACTTACAAAGATGCATTAAAGCATGCAGACTCACCAAACGACTTGCG
TCTCACCATTAAGCTTTCAGAAGAAGGCGCAGATCAACTACTGAATGCGAGCCGTAACATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9GMD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

70.081

99.731

0.699
  pilU Vibrio cholerae strain A1552

54.324

99.462

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

97.849

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment